An Enzymatic Method for the Determination of Free Fatty Acids in Serum/Plasma
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Ahmet Kızıltunç
Abstract
Estimation of serum free fatty acids (FFA) in serum based on the formation of inorganic phosphate has been simplified by eliminating complex stages. The principle of the present method is based on breakdown of pyrophosphate, formed by thioesterification of free fatty acids with ATP and CoA with the aid of acyl-CoA synthetase (EC 6.2.1.3) to inorganic phosphate. This is measured using the reaction with molybdate. The reaction equations are as follows:
The recovery of free fatty acids was 96 %. The interferences of citrate, phosphatidylserine, succinate, ascorbic acid and lecithin were between 0.5 and 2 %. The correlation between the new enzymatic and the classic enzymatic method was 0.966. The lower detection limit was 0.018 mmol/I. The method was linear between 0.02 and 2.0 mmol/I. The within-assay and between-assay imprecision (CV) of control sera was 5.5 % and 8 %, respectively.
Copyright (c) 1998 by Walter de Gruyter GmbH & Co. KG
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Artikel in diesem Heft
- Genetic Approach for Insulin-Independent Diabetes Mellitus in Clinical Practice
- An Enzymatic Method for the Determination of Free Fatty Acids in Serum/Plasma
- Specificity of Cardiac Troponins I and T in Renal Disease
- Blood and Tissue Findings in the Diagnosis of Mesenteric Ischaemia: An Experimental study
- Fucosylation and Galactosylation of IgG Heavy Chains Differ between Acute and Remission Phases of Juvenile Chronic Arthritis
- Analytical Evaluation of an Improved Procedure for Measuring Thyrotropin
- Erroneous Monocyte and Neutrophil Counts with the Sysmex NE-1500 in Patients Recovering from Bone Marrow Aplasia
- N-Benzoyl, L-Glutamic Acid as a Suitable Internal Standard for the Analysis of trans,trans-Muconic Acid in Human Urine by Liquid Chromatography
- Acute Exogenous Elevation of Plasma Free Fatty Acids Does Not Influence the Plasma Magnesium Concentration
- Characterisation and Evaluation of External Quality Assessment Scheme Serum
- Need for Correct Estimates of Biological Variation: The Example of C-Reactive Protein
