Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA
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Arun Kumar Shukla
Abstract
We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable fold-back tetraplex DNA structure under supercoiling. To establish the biological significance of this structure, we inserted this sequence between two direct repeat sequences, separated by 200 bp, in a plasmid. We then investigated the effect of this sequence on homologous recombination events. Here we report that the putative fold-back DNA tetraplex structure induces homologous recombination between direct repeat sequences. Interestingly, this recombination event is independent of recA, a major driving force for homologous recombination. We think that the fold-back structure forces the repeat sequences to come into close proximity and therefore leads to strand exchange. Although triplex-induced recombination has been well documented, our results for the first time directly establish the potential of a tetraplex structure to induce recA-independent homologous recombination in vivo. This finding might have a significant implication for site-directed gene deletion in the context of the correction of genetic defects.
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Artikel in diesem Heft
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Artikel in diesem Heft
- Striated domains: self-organizing ordered assemblies of transmembrane α-helical peptides and lipids in bilayers
- Evolution of kallikrein-related peptidases in mammals and identification of a genetic locus encoding potential regulatory inhibitors
- Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA
- Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation
- DNA end-joining driven by microhomologies catalyzed by nuclear extracts
- A role for transmembrane domains V and VI in ligand binding and maturation of the angiotensin II AT1 receptor
- The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB
- Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A
- Truncated PrPc in mammalian brain: interspecies variation and location in membrane rafts
- Endotoxin-like properties of a rhamnolipid exotoxin from Burkholderia (Pseudomonas) plantarii: immune cell stimulation and biophysical characterization
- p190-RhoGAP as an integral component of the Tiam1/Rac1-induced downregulation of Rho
- Human plasma adenosine deaminase 2 is secreted by activated monocytes
- Inhibition of mRNA deadenylation and degradation by different types of cell stress
- Identification of calpain cleavage sites in the G1 cyclin-dependent kinase inhibitor p19INK4d
- Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8
