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DNA end-joining driven by microhomologies catalyzed by nuclear extracts

  • Francisco Boán , Miguel G. Blanco , Paula Barros and Jaime Gómez-Márquez
Published/Copyright: March 17, 2006
Biological Chemistry
From the journal Volume 387 Issue 3

Abstract

In a previous work we used an in vitro system for the generation and analysis of double-strand breaks (DSBs) using nuclear extracts from rat testes as a source of DSB activity. Since the recombination process can be triggered by the formation of DSB, in the present study we developed a strategy to isolate and characterize recombinant molecules using the same in vitro system. Our results indicate that the mechanism for the formation of recombinants was non-homologous end-joining driven by microhomologies. The procedure described here represents an alternative to investigate the mechanisms of DNA end-joining and other forms of DNA repair.

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Published Online: 2006-03-17
Published in Print: 2006-03-01

©2006 by Walter de Gruyter Berlin New York

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