Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation
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Anna A. Neschastnova
Abstract
Mutation detection and mismatch repair investigations based on heteroduplex formation require a linear DNA structure. DNA branching, described previously under physiological conditions, has been analysed in the heteroduplex formation process. Symmetrical χ-structures were detected after heteroduplex formation by gel electrophoresis and electron microscopy. Buffer composition, DNA concentration and duplex end-sequences influence DNA branching. Duplexes with homologous central regions but non-complementary ends do not form hybrid heteroduplexes or hybrid Holliday junctions. Our results explain the requirements for efficient heteroduplex formation, which were previously determined empirically: special solution composition, optimal DNA concentration and GC clamps. This provides the theoretical background for further optimisation of the procedure.
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Articles in the same Issue
- Striated domains: self-organizing ordered assemblies of transmembrane α-helical peptides and lipids in bilayers
- Evolution of kallikrein-related peptidases in mammals and identification of a genetic locus encoding potential regulatory inhibitors
- Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA
- Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation
- DNA end-joining driven by microhomologies catalyzed by nuclear extracts
- A role for transmembrane domains V and VI in ligand binding and maturation of the angiotensin II AT1 receptor
- The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB
- Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A
- Truncated PrPc in mammalian brain: interspecies variation and location in membrane rafts
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