Human plasma adenosine deaminase 2 is secreted by activated monocytes
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Sachiko Iwaki-Egawa
Abstract
Adenosine deaminase (ADA) plays an important role in the immune system, and its activity is composed of two kinetically distinct isozymes, ADA1 and ADA2. ADA2 is a major component of human plasma total ADA activity and ADA2 activity is significantly elevated in patients with various diseases such as HIV infection and chronic hepatitis. However, relatively little is known about ADA2 because of difficulties in purifying this enzyme. In this study we succeeded in purifying human plasma ADA2 and demonstrate the extracellular secretion of ADA2 from human peripheral blood monocytes stimulated with phorbol 12-myristate 13-acetate and calcium ionophore.
References
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©2006 by Walter de Gruyter Berlin New York
Articles in the same Issue
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- Inhibition of mRNA deadenylation and degradation by different types of cell stress
- Identification of calpain cleavage sites in the G1 cyclin-dependent kinase inhibitor p19INK4d
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Articles in the same Issue
- Striated domains: self-organizing ordered assemblies of transmembrane α-helical peptides and lipids in bilayers
- Evolution of kallikrein-related peptidases in mammals and identification of a genetic locus encoding potential regulatory inhibitors
- Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA
- Spontaneous DNA-DNA interaction of homologous duplexes and factors affecting the result of heteroduplex formation
- DNA end-joining driven by microhomologies catalyzed by nuclear extracts
- A role for transmembrane domains V and VI in ligand binding and maturation of the angiotensin II AT1 receptor
- The zinc finger protein ZNF297B interacts with BDP1, a subunit of TFIIIB
- Pressure- and temperature-induced unfolding studies: thermodynamics of core hydrophobicity and packing of ribonuclease A
- Truncated PrPc in mammalian brain: interspecies variation and location in membrane rafts
- Endotoxin-like properties of a rhamnolipid exotoxin from Burkholderia (Pseudomonas) plantarii: immune cell stimulation and biophysical characterization
- p190-RhoGAP as an integral component of the Tiam1/Rac1-induced downregulation of Rho
- Human plasma adenosine deaminase 2 is secreted by activated monocytes
- Inhibition of mRNA deadenylation and degradation by different types of cell stress
- Identification of calpain cleavage sites in the G1 cyclin-dependent kinase inhibitor p19INK4d
- Identification of candidate substrates for ectodomain shedding by the metalloprotease-disintegrin ADAM8
