Study on the Interaction Between Cellulase and Surfactants

Jian Zhang; Yue Yu

doi:10.3139/113.110493

Article

Study on the Interaction Between Cellulase and Surfactants

Jian Zhang and Yue Yu

Published/Copyright: May 8, 2017

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From the journal Tenside Surfactants Detergents Volume 54 Issue 3

Abstract

The conformations of secondary and tertiary structures of cellulase in the presence of eleven commonly used surfactants were determined by Raman spectroscopy and the results were discussed. The results indicated that anionic surfactants had a stronger influence on the cellulase conformations than nonionic surfactants. Thus anionic surfactants showed a stronger inactivation on the cellulase activity. Furthermore, Zeta potential distributions of cellulase in solutions of surfactants were tested by Dynamic Light Scattering (DLS). The DLS results indicated that the interaction between anionic surfactants and cellulase was attributed to electrostatic attraction. By adding cellulase to a liquid, non-cellulase-containing detergent, the detergency of the liquid detergent could be increased. Further studies on the sample swatches by optical microscopy and scanning electron microscopy (SEM) were undertaken in this paper.

Kurzfassung

Die Konformationen der sekundären und der tertiären Strukturen von Cellulase in Gegenwart von elf häufig verwendeten Tensiden wurden mit Hilfe der Ramanspektroskopie bestimmt und die Ergebnisse diskutiert. Die Ergebnisse zeigten, dass anionische Tenside einen stärkeren Einfluss auf die Cellulase-Konformationen hatten als nichtionische Tenside. Anionische Tenside inaktivierten Cellulase stärker. Darüber hinaus wurden Zetapotentialverteilungen von Cellulase in Tensid-Lösungen mittels dynamischer Lichtstreuung (DLS) bestimmt. Die DLS-Ergebnisse zeigten, dass die Wechselwirkung zwischen anionischen Tensiden und Cellulase von der elektrostatischen Anziehung bestimmt wurde. Durch Hinzugeben von Cellulase in ein flüssiges, nicht cellulasehaltiges Waschmittel konnte die Waschleistung des Waschmittels erhöht werden. Weitere Untersuchungen der Testgewebe mittels optischer Mikroskopie und Rasterelektronenmikroskopie (SEM) wurden in dieser Arbeit durchgeführt.

Key words: Cellulase; surfactants; raman spectroscopy; scanning electron microscopy (DLS); detergency

Stichwörter: Cellulase; Tenside; Ramanspektroskopie; Rasterelektronenmikroskopie (SEM); Waschleistung

*Correspondence address, Prof. Jian Zhang, Shanxi University, School of Chemistry and Chemical Engineering, Taiyuan, Shanxi, China, Tel.: 13834110276, E-Mail: zhangjian0923@vip.sina.com

Jian Zhang is a professor at School of Chemistry and Chemical Engineering, Shanxi University. After she received her PhD in chemistry from Taiyuan University of Technology, she went to Oxford University, Physical and Theoretical Chemistry Laboratory for post-doctoral research program which sponsored by Royal Society U.K. Her research interest lies in detergent enzymes and its application.

Yue Yu is a graduate student at School of Chemistry and Chemical Engineering, Shanxi University.

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Received: 2016-10-28

Accepted: 2017-01-09

Published Online: 2017-05-08

Published in Print: 2017-05-15

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Articles in the same Issue

https://doi.org/10.3139/113.110493

Keywords for this article

Cellulase; surfactants; raman spectroscopy; scanning electron microscopy (DLS); detergency