Startseite Lebenswissenschaften Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
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Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling

Ein Erratum zu diesem Artikel finden Sie hier: https://doi.org/10.1515/znc-2018-0133
  • Cheng Libao EMAIL logo , Jiang Runzhi , Yang Jianjun , Xu Xiaoyong , Zeng Haitao und Li Shuyan EMAIL logo
Veröffentlicht/Copyright: 12. Februar 2018
Zeitschrift für Naturforschung C
Aus der Zeitschrift Zeitschrift für Naturforschung C Band 73 Heft 5-6

Abstract

Adventitious roots (ARs) of lotus (Nelumbonucifera Gaertn.) play a critical role in water and nutrient uptake. We found that exogenously applied 10-μM indole-3-acetic acid (IAA) promoted the formation of ARs, while 150-μM IAA significantly inhibited the emergence of ARs. However, little is known about these different responses to various concentrations of IAA at the molecular level. This study, therefore, examined the gene expression profiling in four libraries treated with 10- and 150-μM IAA based on the high-throughout tag sequencing technique. Approximately 2.4×107 clean tags were obtained after the removal of low-quality tags from each library respectively, among which about 10% clean tags were unambiguous tag-mapped genes to the reference genes. We found that some genes involved in auxin metabolism showed a similar tendency for expression in the A/CK and C/CK libraries, while three genes were enhanced their expression only in the A/CK libraries. Two transcription factors including B3 domain-containing protein At2g36080-like and trihelix transcription factor were up-regulated for transcriptional level in the A/C libraries. The expressions of six important genes related to AR formation were significantly different in the A/CK and C/CK libraries. In summary, this study provides a comprehensive understanding of gene expression regulated by IAA involved in AR formation in lotus.

Keywords: adventitious roots; expression; indole-3-acetic acid (IAA); lotus root; RNA-seq

Acknowledgments

The authors thank some members of BIG for their cooperation in obtaining the data during the AR formation of the lotus by the RNA-seq technique. The authors also thank Edanz Group Ltd. for their editorial assistance. This work was supported by the modern agriculture of Yangzhou (YZ2017044), the Innovation Project of Jiangsu Province and Yangzhou University (SJCX17_0629) and the Natural Science Foundation of Jiangsu Province, China, Funder ID: 10.13039/501100004608 (BK20151307).

  1. Conflict of interest statement: The authors declare no conflict of interest.

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Supplemental Material:

The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2017-0188).

Received: 2017-10-11
Revised: 2017-12-05
Accepted: 2018-01-20
Published Online: 2018-02-12
Published in Print: 2018-04-25

©2018 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
  3. Monoterpenes induce the heat shock response in Arabidopsis
  4. Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
  5. Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
  6. Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
  7. Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
  8. Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
  9. Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
  10. Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
  11. Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
https://doi.org/10.1515/znc-2017-0188
Schlagwörter für diesen Artikel
adventitious roots; expression; indole-3-acetic acid (IAA); lotus root; RNA-seq

Artikel in diesem Heft

  1. Frontmatter
  2. Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
  3. Monoterpenes induce the heat shock response in Arabidopsis
  4. Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
  5. Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
  6. Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
  7. Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
  8. Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
  9. Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
  10. Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
  11. Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
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