Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
-
Christèle Sorèle Mambou
,
Jean-de-Dieu Tamokou
Abstract
The antibacterial-guided investigation of the stem bark extract of Pseudocedrela kotschyi led to the isolation of a new secotirucallane triterpene derivative: 4-hydroxy-3,4-secotirucalla-7,24-dien-3,21-dioic acid (1), together with the known one: 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic acid (2) and 3-methyl ester 3,4-secotirucalla-4(28),7,24-trien-3,21-dioic (3). The structures of the isolated compounds were elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy. Extracts, fractions and compounds (1–3) were tested in vitro for antibacterial activity against two Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus ATCC 25923), and two Gram negative bacteria (Escherichia coli S2(1) and Pseudomonas aeruginosa). The MeOH extract and the Hex/CH2Cl2 (70:30) fraction showed significant levels of activity (MIC=64– 256 μg/mL) compared with the two reference drugs [ciprofloxacin: MIC (0.5–1 μg/mL) and amoxicillin: MIC (1–128 μg/mL)]. Moreover, the compound 2 isolated from this Hex/CH2Cl2 (70:30) fraction had the greatest potential value against S. aureus, E. coli and P. aeruginosa, with minimum inhibitory concentrations (MIC) ranging from 4–16 μg/mL.
Acknowledgments
The authors acknowledge the DAAD for the small-scale equipment provided to J. R. Chouna. They also acknowledge the financial support of the Alango Foundation (African Phytomedecine Center, Dschang, Cameroon).
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Supplemental Material:
The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2017-0207).
©2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
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- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
