Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
-
Kalapad Santosh Prabhu
and
Nilamani Dikshit
Abstract
Finger millet (Eleusine coracana L. Gaertn., 2n=36) is one of the most important minor crops, commonly known as ‘ragi’ and used as a staple food grain in more than 25 countries including Africa and south Asia. Twenty-seven accessions of ragi were collected from different parts of India and were evaluated for morpho-genetic diversity studies. Simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers were used for assessment of genetic diversity among 27 genotypes of E. coracana. High degree of similarity (90%) was obtained between ‘IC49979A’ and ‘IC49974B’ genotypes, whereas low level of similarity (9.09%) was found between ‘IC204141’ and ‘IC49985’ as evident in morphological and DNA markers. A total of 64 SSR and 301 RAPD amplicons were produced, out of which 87.50% and 77.20% DNA fragments showed polymorphism, respectively. The clustering pattern obtained among the genotypes corresponded well with their morphological and cytological data with a monophyletic origin of this species which was further supported by high bootstrap values and principal component analysis. Cluster analysis showed that ragi accessions were categorised into three distinct groups. Genotypes IC344761, IC340116, IC340127, IC49965 and IC49985 found accession specific in RAPD and SSR markers. The variation among ragi accessions might be used as potential source of germplasm for crop improvement.
Acknowledgments
The financial support received by Kalapad Santosh Prabhu under Postgraduate HRD support programme, Department of Biotechnology, Government of India, is highly acknowledged during this work. The financial assistance received for DST-FIST and UGC-DRS-III, Government of India, operating in the Department of Botany, Utkal University, for this study to ABD is acknowledged.
Conflict of interest statement: The authors do not have any conflict of interest.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
- Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
Articles in the same Issue
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
- Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
