Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
-
Amadou Dawé
,
Marius Mbiantcha
Abstract
Despite the well-documented benefits of Combretum fragrans in Cameroon, only few scientific works have been done on it. In this study we isolated eight compounds from the leaves extract of C. fragrans: velutin (1), belamcanidin (2), cirsilineol (3), cirsimaritin (4), 3β-acetoxy-20,24-epoxy-11,25-hydroxy-dammarane (5), combretin A (6), combretin B (7) and a mixture of arjunolic acid (8a) and asiatic acid (8b). Compounds 6 and 7 presented potent anti-inflammatory, antioxidant and antidiabetic activities. Compounds 1, 3, 5 and the mixture of 8a and 8b were significantly active, and compounds 2 and 4 presented moderate activity for reactive oxygen species inhibitory and free-radical scavenging. All compounds were isolated using chromatographic techniques; their structures were elucidated by spectroscopic techniques and their spectroscopic data compared with those of the literature. Anti-inflammatory activity was evaluated via the oxidative burst assay using a luminol-amplified chemiluminescence technique, antioxidant activity by free-radical scavenging activity (DPPH) and antidiabetic activity via α-glucosidase inhibition. All of the isolated compounds (1–8) were reported to exhibit significant antioxidant activity. Compounds 1, 3, and 5–8 exhibited potent chemiluminescence inhibition effect, and only compounds 6 and 7 inhibited α-glucosidase. Thus, C. fragrans can be used as an effective natural source of anti-inflammatory, antioxidant and antidiabetic compounds.
Acknowledgments
Authors are very grateful to The World Academy of Sciences (TWAS) and the International Center for Chemical and Biological Sciences (ICCBS), University of Karachi, Pakistan, for their financial support, facilities and technical support through the ICCBS-TWAS Postdoctoral Fellowship number 3240280473 granted to Dr Dawe.
Declarations
Ethics approval and consent to participate: For the donation of human blood samples, all processes of collecting blood was accepted and approved by independent Ethics Committee, ICCBS, University of Karachi, No.: ICCBS/IEC-008-BC-2015/Protocol/1.0. The blood donors were provided information for approval of the use of their blood for the purposes of this study. Animal facility was provided by ICCBS, University of Karachi, and experiments were executed under the ethical guidelines of the International Association for the Study of Pain in Conscious Animals; guidelines set by the scientific advisory committee, animal care, use and standards, ICCBS were followed (Protocol no. 1209004).
Availability of data and materials: All data supporting our findings are included in this published article.
Conflict of interest: We wish to confirm that there are no known conflicts of interest associated with this publication, and there has been no significant financial support for this work that could have influenced its outcome.
Funding: This manuscript research project was supported by the TWAS (Academy of Science of Developing Countries) and ICCBS (International Center for Chemical and Biological Sciences), University of Karachi.
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Supplemental Material:
The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2017-0166).
©2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
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- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
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- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
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Artikel in diesem Heft
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
- Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
