Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
-
Vesselin Kussovski
,
Mahmut Durmuş
Abstract
Photodynamic inactivation (PDI) has been featured as an effective strategy in the treatment of acute drug-resistant infections. The efficiency of PDI was evaluated against three periodontal pathogenic bacteria that were tested as drug-resistant strains. In vitro studies were performed with four water-soluble cationic Zn(II) phthalocyanines (ZnPc1–4) and irradiation of a specific light source (light-emitting diode, 665 nm) with three doses (15, 36 and 60 J/cm2). The well detectable fluorescence of ZnPcs allowed the cellular imaging, which suggested relatively high uptakes of ZnPcs into bacterial species. Complete photoinactivation was achieved with all studied ZnPc1–4 for Enterococcus faecalis (E. faecalis) at a light dose of 15 J/cm2. The photodynamic response was high for Prevotella intermedia (P. intermedia) after the application of 6 μM of ZnPc1 and a light dose of 36 J/cm2 and for 6 μM of ZnPc2 at 60 J/cm2. P. intermedia was inactivated with ZnPc3 (4 log) and ZnPc4 (2 log) with irradiation at an optimal dose of 60 J/cm2. Similar photoinactivation results (2 log) were achieved for Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) treated with 6 μM ZnPc1 and ZnPc2 at a light dose of 60 J/cm2. The study suggested that PDI with quaternized Zn(II) phthalocyanines and specific light irradiation appears to be a very useful antimicrobial strategy for effective inactivation of drug-resistant periodontal pathogens.
Acknowledgments
The authors thank Prof. Slavcho Dimitrov, MD, for discussions. The support made by the National Science Fund (Funder Id: 10.13039/501100003336, grants DO-02-177/08 and B02/9/2014).
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
- Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
Articles in the same Issue
- Frontmatter
- Assessment of genetic diversity in ragi [Eleusine coracana (L.) Gaertn] using morphological, RAPD and SSR markers
- Monoterpenes induce the heat shock response in Arabidopsis
- Mangostanaxanthone VII, a new cytotoxic xanthone from Garcinia mangostana
- Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum)
- Bioactive pyrrole alkaloids isolated from the Red Sea: marine sponge Stylissa carteri
- Flavonoids and triterpenes from Combretum fragrans with anti-inflammatory, antioxidant and antidiabetic potential
- Quaternized Zn(II) phthalocyanines for photodynamic strategy against resistant periodontal bacteria
- Transcriptome profiling reveals an IAA-regulated response to adventitious root formation in lotus seedling
- Antibacterial secotirucallane triterpenes from the stem bark of Pseudocedrela kotschyi
- Endophytic Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth
