Home Medicine The use of micronucleus assay in oral mucosa cells as a suitable biomarker in children exposed to environmental mutagens: theoretical concepts, guidelines and future directions
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The use of micronucleus assay in oral mucosa cells as a suitable biomarker in children exposed to environmental mutagens: theoretical concepts, guidelines and future directions

  • Ingra Tais Malacarne , Maria Esther Suarez Alpire , Andrea Cristina de Moraes Malinverni and Daniel Araki Ribeiro EMAIL logo
Published/Copyright: November 11, 2022
Reviews on Environmental Health
From the journal Reviews on Environmental Health Volume 39 Issue 2

Abstract

In the last decades, the micronucleus assay has been recognized as a suitable biomarker for monitoring populations exposed to many different occupational factors, lifestyle, environmental conditions, radiation exposure, and deleterious effects of pesticides. The objective of this work is to direct the design of future field studies in the assessment of the risk of children exposed to environmental mutagens, radiation, and pesticides. This review sought available information on the analysis of micronuclei in oral cells in children. A literature search for papers investigating DNA damage, genetic damage, oral cells, buccal cells, genotoxicity, mutagenicity and micronucleus was begun in 2000 and is scheduled to be concluded in May, 2022. Briefly, a search of PubMed, MEDLINE, and Google Scholar for a variety of articles was performed. The results showed that there are still few studies that addressed micronuclei of oral cells in children exposed to the most diverse environmental conditions. Only environmental pollution was associated with damage to the genome of oral cells in children. Therefore, researchers need to be calibrated in cell analysis, standardization of field study protocols and the development of new research in the evaluation of children using the micronucleus test as a tool in child biomonitoring.

Keywords: children; environmental mutagen; micronucleus assay; oral mucosa
Corresponding author: Prof. Dr. Daniel Araki Ribeiro, Department of Biosciences, Institute of Health and Society, Federal University of Sao Paulo, UNIFESP, Rua Silva Jardim, 136, Room 332, Vila Mathias, Santos – SP, 11050-020, Brazil, Phone: +55 1332290156, E-mail:

Funding source: Conselho Nacional de Desenvolvimento CientÃ-fico e Tecnológico

Award Identifier / Grant number: 001

  1. Research funding: DAR thanks CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnológico, grant number #001) for productivity fellowships.

  2. Author contributions: Study design: ITM and DAR. Data search: ITM, and MESA. Data analysis: ITM and DAR. Writing the paper: ITM, MESA and DAR.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Not applicable.

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Received: 2022-06-01
Accepted: 2022-10-25
Published Online: 2022-11-11
Published in Print: 2024-06-25

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. The lack of international and national health policies to protect persons with self-declared electromagnetic hypersensitivity
  4. The use of micronucleus assay in oral mucosa cells as a suitable biomarker in children exposed to environmental mutagens: theoretical concepts, guidelines and future directions
  5. Improving the purification of aqueous solutions by controlling the production of reactive oxygen species in non-thermal plasma; a systematic review
  6. Ochratoxin A in coffee and coffee-based products: a global systematic review, meta-analysis, and probabilistic risk assessment
  7. Green space in health research: an overview of common indicators of greenness
  8. The effects of fine particulate matter on the blood-testis barrier and its potential mechanisms
  9. Evaluation of chemicals leached from PET and recycled PET containers into beverages
  10. The association between bisphenol a exposure and attention deficit hyperactivity disorder in children: a meta-analysis of observational studies
  11. A review on arsenic pollution, toxicity, health risks, and management strategies using nanoremediation approaches
  12. The impact of air pollution and climate change on eye health: a global review
  13. Exposure to Polycyclic Aromatic Hydrocarbons and adverse reproductive outcomes in women: current status and future perspectives
  14. Mechanisms of cholera transmission via environment in India and Bangladesh: state of the science review
  15. Effects of sulfur dioxide inhalation on human health: a review
  16. Health effects of alkaline, oxygenated, and demineralized water compared to mineral water among healthy population: a systematic review
  17. Toxic effects due to exposure heavy metals and increased health risk assessment (leukemia)
  18. A systematic review on environmental perspectives of monkeypox virus
  19. How does formal and informal industry contribute to lead exposure? A narrative review from Vietnam, Uruguay, and Malaysia
  20. Letter to the Editor
  21. Comments on “Personal protective equipment (PPE) and plastic pollution during COVID-19: strategies for a sustainable environment”, by Fatima Ali Mazahir and Ali Mazahir Al Qamari
https://doi.org/10.1515/reveh-2022-0084
Keywords for this article
children; environmental mutagen; micronucleus assay; oral mucosa

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. The lack of international and national health policies to protect persons with self-declared electromagnetic hypersensitivity
  4. The use of micronucleus assay in oral mucosa cells as a suitable biomarker in children exposed to environmental mutagens: theoretical concepts, guidelines and future directions
  5. Improving the purification of aqueous solutions by controlling the production of reactive oxygen species in non-thermal plasma; a systematic review
  6. Ochratoxin A in coffee and coffee-based products: a global systematic review, meta-analysis, and probabilistic risk assessment
  7. Green space in health research: an overview of common indicators of greenness
  8. The effects of fine particulate matter on the blood-testis barrier and its potential mechanisms
  9. Evaluation of chemicals leached from PET and recycled PET containers into beverages
  10. The association between bisphenol a exposure and attention deficit hyperactivity disorder in children: a meta-analysis of observational studies
  11. A review on arsenic pollution, toxicity, health risks, and management strategies using nanoremediation approaches
  12. The impact of air pollution and climate change on eye health: a global review
  13. Exposure to Polycyclic Aromatic Hydrocarbons and adverse reproductive outcomes in women: current status and future perspectives
  14. Mechanisms of cholera transmission via environment in India and Bangladesh: state of the science review
  15. Effects of sulfur dioxide inhalation on human health: a review
  16. Health effects of alkaline, oxygenated, and demineralized water compared to mineral water among healthy population: a systematic review
  17. Toxic effects due to exposure heavy metals and increased health risk assessment (leukemia)
  18. A systematic review on environmental perspectives of monkeypox virus
  19. How does formal and informal industry contribute to lead exposure? A narrative review from Vietnam, Uruguay, and Malaysia
  20. Letter to the Editor
  21. Comments on “Personal protective equipment (PPE) and plastic pollution during COVID-19: strategies for a sustainable environment”, by Fatima Ali Mazahir and Ali Mazahir Al Qamari
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