Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering
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Mohd Syahir Anwar Hamzah
,
Mohammed Rafiq Abdul Kadir
Abstract
This work reports the fabrication and characterizations of porous scaffold made up of polylactic acid (PLA) with the inclusion of pectin (1, 3, 5, 7, 9, 11 wt%) for potential tissue engineering material. The composite scaffold was prepared using a facile method of freeze extraction. Based on the physical evaluations, the scaffold was suggested to be optimum at 5 wt% of pectin loading. Water contact angle of the scaffold was significantly reduced to 46.5o with the inclusion of 5 wt% of pectin. Morphological and topographic of the PLA scaffold revealed that the pectin induced more porous structure and its surface became rougher which was suitable for cell attachment and proliferation. In vitro studies of the PLA/pectin composite scaffold using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidelt (MTT) assay revealed good biocompatibility whereas Live-Dead kit assay resulted in 91% cell viability after 7 days of incubation.
Funding source: Universiti Teknologi Malaysia
Award Identifier / Grant number: 03G83, 04G53
Research funding: Financial support from the Universiti Teknologi Malaysia grant number 04G53 and 03G83 are gratefully acknowledged.
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© 2020 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Material properties
- Structure-properties relationship for energy storage redox polymers: a review
- Effects of chain polarity of hindered phenol on the damping properties of polymer-based hybrid materials: insights into the molecular mechanism
- Effect of interfacial modification on the thermo-mechanical properties of flax reinforced polylactide stereocomplex composites
- Use of diisocyanate to enhance the flame-retardant, mechanical and crystalline properties of poly (butylene succinate-co-butylene 3-hydroxyphenylphosphinyl-propionate) (PBSH)
- Preparation and assembly
- Graphene oxide modified carbon fiber reinforced epoxy composites
- Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering
- Engineering and processing
- Study on the interface morphology in the induction welding joint of PEEK plate at low power
- Ionic gelated β-cyclodextrin-biotin-carboxymethyl chitosan nanoparticles prepared as carrier for oral delivery of protein drugs
Artikel in diesem Heft
- Frontmatter
- Material properties
- Structure-properties relationship for energy storage redox polymers: a review
- Effects of chain polarity of hindered phenol on the damping properties of polymer-based hybrid materials: insights into the molecular mechanism
- Effect of interfacial modification on the thermo-mechanical properties of flax reinforced polylactide stereocomplex composites
- Use of diisocyanate to enhance the flame-retardant, mechanical and crystalline properties of poly (butylene succinate-co-butylene 3-hydroxyphenylphosphinyl-propionate) (PBSH)
- Preparation and assembly
- Graphene oxide modified carbon fiber reinforced epoxy composites
- Fabrication and evaluation of polylactic acid/pectin composite scaffold via freeze extraction for tissue engineering
- Engineering and processing
- Study on the interface morphology in the induction welding joint of PEEK plate at low power
- Ionic gelated β-cyclodextrin-biotin-carboxymethyl chitosan nanoparticles prepared as carrier for oral delivery of protein drugs
