Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
-
Mohammad Mahdi Safikhani
,
Farnaz Ghorbani
Abstract
Tissue engineering is a biotechnology that is used to develop biological substitutes to restore, maintain, or improve functions. Thus, the porous scaffolds are used to accommodate cells in tissue engineering. In this research, three dimensional (3D) bi-layered polyurethane (PU)-gelatin nanofibrous scaffolds were prepared by the electrospinning method, after which the capability of the released heparin as an anti-coagulation factor was evaluated. Electrospinning has been extensively investigated for the preparation of fibers that exhibit a high surface area to volume ratio. Results showed that scanning electron microscopy (SEM) micrographs exhibited a smooth surface as well as a highly porous and bead-free structure, in which fibers were distributed in the range of 100–600 nm. The modulus and ultimate tensile strength (UTS) decreased and increased, respectively, after crosslinking the reaction of polymers. This process also reduced swelling ratio, the hydrolytic biodegradation rate, and the release rate as a function of time. Moreover, an in vitro assay demonstrated that 3D nanofibrous scaffolds supported L929 fibroblast cell viability and that cells adhered and spread on the fibers. Based on the obtained results, the heparin-loaded electrospinning nanofibrous scaffolds have initial physicochemical and mechanical properties to protect neo-tissue formation.
References
[1] Druy MD. J. Biomaterial. 2003, 24, 4337.10.1016/S0142-9612(03)00340-5Suche in Google Scholar
[2] Cao H, Liu T, Chew SY. Adv. Drug Deliv. Rev. 2009, 61, 1055.10.1016/j.addr.2009.07.009Suche in Google Scholar
[3] Kucińska-Lipka J, Gubańska I, Janik H. ScientificWorldJ. 2013, 2013, 450132.10.1155/2013/450132Suche in Google Scholar
[4] Ghorbani F, Nojehdehyan H, Zamanian A, Gholipourmalekabadi M, Mozafari M. Adv. Mater. Lett. 2016, 7, 163.10.5185/amlett.2016.6003Suche in Google Scholar
[5] Unnithan AR, Arathyram RS, Kim CS. Electrospinning of Polymers for Tissue Engineering, Elsevier Inc. 2015.10.1016/B978-0-323-32889-0.00003-0Suche in Google Scholar
[6] Ghazanfari SMH, Zamanian A. Ceram. Int. 2013, 39, 9835.10.1016/j.ceramint.2013.05.096Suche in Google Scholar
[7] Arabi N, Zamanian A. Biotechnol. Appl. Biochem. 2013, 60, 573.10.1002/bab.1120Suche in Google Scholar
[8] Pourhaghgouy M, Zamanian A, Shahrezaee M, Masouleh MP. Mater. Sci. Eng. 2016, 58, 180.10.1016/j.msec.2015.07.065Suche in Google Scholar
[9] Zamanian A, Farhangdoust S, Yasaei M, Khorami M, Hafezi M. Int. J. Appl. Ceram. Technol. 2014, 11, 12.10.1111/ijac.12031Suche in Google Scholar
[10] Zamanian A, Ghorbani F, Nojehdehian H. Appl. Mech. Mater. 2013, 467, 108.10.4028/www.scientific.net/AMM.467.108Suche in Google Scholar
[11] Yoon JJ, Kim JH, Park TG. Biomaterials 2003, 24, 2323.10.1016/S0142-9612(03)00024-3Suche in Google Scholar
[12] Asefnejad A, Khorasani MT, Behnamghader A, Farsadzadeh B, Bonakdar S. Int. J. Nanomed. 2011, 6, 2375.10.2147/IJN.S15586Suche in Google Scholar PubMed PubMed Central
[13] Pham QP, Sharma U, Mikos AG. Tissue Eng. 2006, 12, 1197.10.1089/ten.2006.12.1197Suche in Google Scholar PubMed
[14] Pickett AN. Elelctrospinning Applications in Mechanochemistry and Multi-Functional Hydrogel Materials, University of Illinois at Urbana Champaign: IL, USA, 2012.Suche in Google Scholar
[15] Montini Ballarin F, Caracciolo PC, Blotta E, Ballarin VL, Abraham GA. Mater. Sci. Eng. 2014, 42, 489.10.1016/j.msec.2014.05.074Suche in Google Scholar PubMed
[16] Rockwood DN, Woodhouse KA, Fromstein JD, Chase DB, Rabolt JF. J. Biomater. Sci. Polym. Ed. 2007, 18, 743.10.1163/156856207781034115Suche in Google Scholar PubMed
[17] Abdolmaleki M, Tavakoli T, Jazani OM, Saeb MR. J. Polym. Eng. 2016, 36, 5, 513.10.1515/polyeng-2015-0254Suche in Google Scholar
[18] Xu Y, Guan J. Advances in Polyurethane Biomaterials, Elsevier: Amsterdam, Netherlands, 2016.Suche in Google Scholar
[19] Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, Nasr-Esfahani M-H, Ramakrishna S. Biomaterials 2008, 29, 4532.10.1016/j.biomaterials.2008.08.007Suche in Google Scholar PubMed
[20] Luong-Van E, Grøndahl L, Chua KN, Leong KW, Nurcombe V, Cool SM. Biomaterials 2006, 27, 2042.10.1016/j.biomaterials.2005.10.028Suche in Google Scholar PubMed
[21] Rowlands AS, Lim SA, Martin D, Cooper-White JJ. Biomaterials 2007, 28, 2109.10.1016/j.biomaterials.2006.12.032Suche in Google Scholar PubMed
[22] Gao C, Guan J, Zhu Y, Shen J. Macromol. Biosci. 2003, 3, 157.10.1002/mabi.200390020Suche in Google Scholar
[23] Chen X, Ergun A, Gevgilili H, Ozkan S, Kalyon DM, Wang H. Biomaterials 2013, 34, 8203.10.1016/j.biomaterials.2013.07.035Suche in Google Scholar PubMed
[24] Ghorbani F, Nojehdehian H, Zamanian A. Mater. Sci. Eng. 2016, 69, 208.10.1016/j.msec.2016.06.079Suche in Google Scholar
[25] Meng ZX, Wang YS, Ma C, Zheng W, Li L, Zheng YF. Mater. Sci. Eng. 2010, 30, 1204.10.1016/j.msec.2010.06.018Suche in Google Scholar
[26] Cai Q. Biomaterials 2003, 24, 629.10.1016/S0142-9612(02)00377-0Suche in Google Scholar
[27] Li X, Cai S, Liu B, Xu Z, Dai X, Ma K, Li S, Yang L, Sung KLP, Fu X. Colloids Surfaces B Biointerf. 2007, 57, 198.10.1016/j.colsurfb.2007.02.010Suche in Google Scholar PubMed
[28] Meng ZX, Xu XX, Zheng W, Zhou HM, Li L, Zheng YF, Lou X. Colloids Surf. B. Biointerf. 2011, 84, 97.10.1016/j.colsurfb.2010.12.022Suche in Google Scholar
[29] Marin GB. Advances in Chemical Engineering: Multiscale Analysis, Academic Press: MA, USA, 2016.Suche in Google Scholar
[30] O’Brien FJ. Mater. Today. 2011, 14, 88.10.1016/S1369-7021(11)70058-XSuche in Google Scholar
[31] Solouk A, Cousins BG, Mirzadeh H, Seifalian AM. Biotechnol. Appl. Biochem. 2011, 58, 311.10.1002/bab.50Suche in Google Scholar PubMed
[32] Hmdi O-HD, Pradhan KC, Nayak PL. Adv. Appl. Sci. 2012, 3, 3045.Suche in Google Scholar
[33] Harada NS, Oyama HT, Bártoli JR, Gouvêa D, Cestari IA, Wang SH. Polym. Int. 2005, 54, 209.10.1002/pi.1685Suche in Google Scholar
[34] Swaminathan S. Artificial Blood Vessels, SASTRA University: Thanjavur, India, 2014.Suche in Google Scholar
[35] Li X-K, Cai S-X, Liu B, Xu Z-L, Dai X-Z, Ma K-W, Lin S-Q, Li S-Q, Yang L, Sung KLP, Fu X-B. Colloids Surf. B. Biointerf. 2007, 57, 198.10.1016/j.colsurfb.2007.02.010Suche in Google Scholar
[36] Tiwari A, Grailer JJ, Pilla S, Steeber DA, Gong S. Acta Biomater. 2009, 5, 3441.10.1016/j.actbio.2009.06.001Suche in Google Scholar PubMed
[37] Adhikari R, Danon SJ, Bean P, Le T, Gunatillake P, Ramshaw JAM, Werkmeister JA. J. Mater. Sci. Mater. Med. 2010, 21, 1081.10.1007/s10856-009-3955-2Suche in Google Scholar PubMed
[38] Meng ZX, Zeng QT, Sun ZZ, Xu XX, Wang YS, Zheng W, Zheng YF. Colloids Surf. B. Biointerf. 2012, 94, 44.10.1016/j.colsurfb.2012.01.017Suche in Google Scholar PubMed
[39] Wu D, Ming W, van Benthem RATM, de With G. J. Adhes. Sci. Technol. 2008, 22, 1869.10.1163/156856108X320023Suche in Google Scholar
[40] Dhandayuthapani B, Yoshida Y, Maekawa T, Kumar DS. Int. J. Polym. Sci. 2011, 2011, 1.10.1155/2011/290602Suche in Google Scholar
[41] Fromstein JD, Zandstra PW, Alperin C, Rockwood D, Rabolt JF, Woodhouse KA. Tissue Eng. Part A 2008, 14, 369.10.1089/tea.2006.0410Suche in Google Scholar PubMed
[42] Wu X, Liu Y, Li X, Wen P, Zhang Y, Long Y, Wang X, Guo Y, Xing F, Gao J. Acta Biomater. 2010, 6, 1167.10.1016/j.actbio.2009.08.041Suche in Google Scholar PubMed
[43] Tiwari A, Sharma Y, Hattori S, Terada D, Sharma AK, Turner APF, Kobayashi H. Biopolymers 2013, 99, 334.10.1002/bip.22170Suche in Google Scholar PubMed
©2017 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Preparation and processing
- Cellulose modification and shaping – a review
- The effect of shear history on urea containing gliadin solutions
- Preparation and characterization of poly(ethylene 2,5-furandicarboxylate/nanocrystalline cellulose composites via solvent casting
- Material properties
- Mechanical properties of natural fibre polymer composites
- Structure and properties of poly(lactic acid)/poly(lactic acid)-α-cyclodextrin inclusion compound composites
- Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations
- Matrix impact on the mechanical, thermal and electrical properties of microfluidized nanofibrillated cellulose composites
- Engineering
- Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
- Fabrication of porous polymeric structures using a simple sonication technique for tissue engineering
Artikel in diesem Heft
- Frontmatter
- Preparation and processing
- Cellulose modification and shaping – a review
- The effect of shear history on urea containing gliadin solutions
- Preparation and characterization of poly(ethylene 2,5-furandicarboxylate/nanocrystalline cellulose composites via solvent casting
- Material properties
- Mechanical properties of natural fibre polymer composites
- Structure and properties of poly(lactic acid)/poly(lactic acid)-α-cyclodextrin inclusion compound composites
- Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations
- Matrix impact on the mechanical, thermal and electrical properties of microfluidized nanofibrillated cellulose composites
- Engineering
- Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
- Fabrication of porous polymeric structures using a simple sonication technique for tissue engineering
