Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
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Mohammad Mahdi Safikhani
,
Farnaz Ghorbani
Abstract
Tissue engineering is a biotechnology that is used to develop biological substitutes to restore, maintain, or improve functions. Thus, the porous scaffolds are used to accommodate cells in tissue engineering. In this research, three dimensional (3D) bi-layered polyurethane (PU)-gelatin nanofibrous scaffolds were prepared by the electrospinning method, after which the capability of the released heparin as an anti-coagulation factor was evaluated. Electrospinning has been extensively investigated for the preparation of fibers that exhibit a high surface area to volume ratio. Results showed that scanning electron microscopy (SEM) micrographs exhibited a smooth surface as well as a highly porous and bead-free structure, in which fibers were distributed in the range of 100–600 nm. The modulus and ultimate tensile strength (UTS) decreased and increased, respectively, after crosslinking the reaction of polymers. This process also reduced swelling ratio, the hydrolytic biodegradation rate, and the release rate as a function of time. Moreover, an in vitro assay demonstrated that 3D nanofibrous scaffolds supported L929 fibroblast cell viability and that cells adhered and spread on the fibers. Based on the obtained results, the heparin-loaded electrospinning nanofibrous scaffolds have initial physicochemical and mechanical properties to protect neo-tissue formation.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Preparation and processing
- Cellulose modification and shaping – a review
- The effect of shear history on urea containing gliadin solutions
- Preparation and characterization of poly(ethylene 2,5-furandicarboxylate/nanocrystalline cellulose composites via solvent casting
- Material properties
- Mechanical properties of natural fibre polymer composites
- Structure and properties of poly(lactic acid)/poly(lactic acid)-α-cyclodextrin inclusion compound composites
- Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations
- Matrix impact on the mechanical, thermal and electrical properties of microfluidized nanofibrillated cellulose composites
- Engineering
- Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
- Fabrication of porous polymeric structures using a simple sonication technique for tissue engineering
Articles in the same Issue
- Frontmatter
- Preparation and processing
- Cellulose modification and shaping – a review
- The effect of shear history on urea containing gliadin solutions
- Preparation and characterization of poly(ethylene 2,5-furandicarboxylate/nanocrystalline cellulose composites via solvent casting
- Material properties
- Mechanical properties of natural fibre polymer composites
- Structure and properties of poly(lactic acid)/poly(lactic acid)-α-cyclodextrin inclusion compound composites
- Fabrication of random and aligned-oriented cellulose acetate nanofibers containing betamethasone sodium phosphate: structural and cell biocompatibility evaluations
- Matrix impact on the mechanical, thermal and electrical properties of microfluidized nanofibrillated cellulose composites
- Engineering
- Bi-layered electrospun nanofibrous polyurethane-gelatin scaffold with targeted heparin release profiles for tissue engineering applications
- Fabrication of porous polymeric structures using a simple sonication technique for tissue engineering
