Prolonged protein immobilization of biosensor by chemically cross-linked glutaraldehyde on mixed cellulose membrane
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Roswani Shaimi
Abstract
Protein immobilization studies with high protein binding sensitivity are of primary concern for any bio-sensing applications. In the present study, glutaraldehyde (GA) was utilized to chemically cross-link with the cellulose compound in mixed cellulose (MC) membrane and the protein molecules. Optimal cross-linking process on membrane was determined statistically and corresponded responses of protein immobilization were described through a quadratic empirical model. Field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) micrographs and thermogravimetric analysis (TGA) have demonstrated the successful deposition of GA on membranes. The optimum GA integration process condition was found at 3 wt% of GA on a single layer and 30 min of integration time, with protein binding predicted at 896.6 μg/cm3. The experimental validation was conducted at this optimum condition, and the result was found at 911.9 μg/cm3, with standard deviation of 1.6%. This small error confirms the adequacy of the empirical model and its ability to predict the GA-protein immobilization performances.
Acknowledgments
The authors are grateful for the financial support provided by the MOHE-FRGS grant (6071251) and the USM Membrane Science and Technology Cluster (8610012). R. Shaimi is financially assisted by the Ministry of Higher Education (MOHE).
References
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Original articles
- Prolonged protein immobilization of biosensor by chemically cross-linked glutaraldehyde on mixed cellulose membrane
- Hybrid biocomposites from agricultural residues: mechanical, water absorption and tribological behaviors
- Effects of nucleation and stereocomplex formation of poly(lactic acid)
- Preparation and characterization of polystyrene-MgAl layered double hydroxide nanocomposites using bulk polymerization
- Fracture behavior and deformation mechanisms of polypropylene/ethylene-propylene-diene blends
- Effect of mechanical properties of metal powder-filled hybrid moulded products
- Ablation and thermo-mechanical tailoring of EPDM rubber using carbon fibers
- Effects of mechanical strength, working temperature and wax lubricant on tribological behavior of polystyrene
- Temperature rise in a verging annular die
- Numerical investigation of the temperature influence on the melt predistribution in a spiral mandrel die with different polyolefins
Articles in the same Issue
- Frontmatter
- Original articles
- Prolonged protein immobilization of biosensor by chemically cross-linked glutaraldehyde on mixed cellulose membrane
- Hybrid biocomposites from agricultural residues: mechanical, water absorption and tribological behaviors
- Effects of nucleation and stereocomplex formation of poly(lactic acid)
- Preparation and characterization of polystyrene-MgAl layered double hydroxide nanocomposites using bulk polymerization
- Fracture behavior and deformation mechanisms of polypropylene/ethylene-propylene-diene blends
- Effect of mechanical properties of metal powder-filled hybrid moulded products
- Ablation and thermo-mechanical tailoring of EPDM rubber using carbon fibers
- Effects of mechanical strength, working temperature and wax lubricant on tribological behavior of polystyrene
- Temperature rise in a verging annular die
- Numerical investigation of the temperature influence on the melt predistribution in a spiral mandrel die with different polyolefins
