Startseite Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
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Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh

  • Azabadji Ashu Evariste Leonce , Priyanka Devi , Tagne Simo Richard , Biswajit Panda , Geetanjali Devabattula , Chandraiah Godugu und Telefo Phelix Bruno EMAIL logo
Veröffentlicht/Copyright: 3. November 2023
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 21 Heft 1

Abstract

Objectives

Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engler.) Van Tiegh are African mistletoe traditionally used in cancers treatment. Thus, the aim of the study was to assess the anti-melanoma potential of the methanol extract of Phragmenthera capitata (Spreng.) Balle (PCMe-OH) and Globimetula braunii (Engler.) (GBMe-OH) Van Tiegh.

Methods

Antioxidant potential was evaluated using DPPH, FRAP and hydroxyl assays. Total flavonoid and phenolic contents was also determined. MTT assay was used to estimate the effects on cell viability using SK-MLE28 and B16-F10 cell lines. Colony formation and wound healing were also assessed. Fluorometry methods were used for qualitative analysis of apoptosis and estimate ROS production. Western blot analysis was used for protein expression.

Results

Phragmenthera capitata (PCMe-OH) showed the highest antioxidant activity and possess the highest phenolic contents (1,490.80 ± 55 mgGAE/g extract) in comparison with G. braunii (GBMe-OH) and (1,071.40 ± 45 mgGAE/g extract). Flavonoid content was similar in both extracts (11.63 ± 5.51 mg CATE/g of extract and 12.46 ± 2.58 mg CATE/g of extract respectively). PC-MeOH showed the highest cytotoxicity effect (IC50 of 55.35 ± 1.17 μg/mL) and exhibited anti-migrative potential on B16-F10 cells. Furthermore, PC-MeOH at 55.35 and 110.7 μg/mL; promoted apoptosis-induced cell death in B16-F10 cells by increasing intracellular ROS levels and reducing Bcl-2 expression level at 110.7 μg/mL. Significant upregulation of P-PTEN expression was recorded with PC-MeOH at 110.7 μg/mL; inhibiting therefore PI3K/AKT/m-Tor signaling pathway. Moreover, at 55.37 μg/mL significant reduction of c-myc and cyclin D1 was observed; dysregulating the MAPK kinase signaling pathway and cell cycle progression.

Conclusions

Phragmenthera capitata may be developed into selective chemotherapy to fight against melanoma.

Keywords: Phragmenthera capitata ; anti-melanoma; apoptosis; MAPK kinase
Corresponding author: Telefo Phelix Bruno, Department of Biochemistry, Dschang University, Research Unit of Biochemistry of Medicinal Plants, Food Sciences and Nutrition, P.O Box 67, Dschang, Cameroon, E-mail:

Acknowledgments

The authors gratefully acknowledge The World Academy of Science (TWAS) and Department of Biotechnology (DBT) fellowship program for financial support, through grant number: 3240269308, and the collaborators for their technical assistance.

  1. Research ethics: The local Institutional Review Board deemed the study exempt from review.

  2. Informed consent: Not applicable.

  3. Author contributions: Telefo Phelix Bruno, Chandraiaih Godugu and Tagne Simo Richard, designed the study. Azabadji Ashu performed the experimental work. Azabadji Ashu and Tagne Simo Richard wrote the manuscript. Biswajit Panda and Geetanjali Devabattulamaintained the cells, Priyanka Devi prepare cell culture media and the required materials. All authors have reviewed the manuscript and approved its submission.

  4. Competing interests: Authors state no conflict of interest.

  5. Research funding: The World Academy of Science (TWAS) and Department of Biotechnology (DBT) of India.

  6. Data availability: Not applicable.

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Received: 2023-08-22
Accepted: 2023-10-03
Published Online: 2023-11-03

© 2023 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Markhamia lutea leaves aqueous and ethanolic extract with curative anti-inflammatory activity attenuates paclitaxel toxicity in rat’s intestine
  4. Does hydrotherapy influence plasma glucose levels in type 2 diabetes? – A scoping review
  5. Comparative effectiveness is the common denominator in health services research: experimental effects are promising, real-world effects are compelling
  6. Research Articles
  7. Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats
  8. Pharmacokinetic assessment of rifampicin and des-acetyl rifampicin in carbon tetrachloride induced liver injury model in Wistar rats
  9. Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
  10. Selenium prevented renal tissue damage in lipopolysaccharide-treated rats
  11. Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line
  12. Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6
  13. Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats
  14. Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
  15. The assessment of the mechanism of action of lauric acid in the context of oral cancer through integrative approach combining network pharmacology and molecular docking technology
  16. Therapeutic potential of flavonoids in ovalbumin induced asthma in mice model
  17. The effects of pioglitazone and rosiglitazone on liver function in hypothyroid rats
  18. Effect of ozone gas on viral kinetics and liver histopathology in hepatitis C patients
https://doi.org/10.1515/jcim-2023-0225
Schlagwörter für diesen Artikel
Phragmenthera capitata; anti-melanoma; apoptosis; MAPK kinase

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Markhamia lutea leaves aqueous and ethanolic extract with curative anti-inflammatory activity attenuates paclitaxel toxicity in rat’s intestine
  4. Does hydrotherapy influence plasma glucose levels in type 2 diabetes? – A scoping review
  5. Comparative effectiveness is the common denominator in health services research: experimental effects are promising, real-world effects are compelling
  6. Research Articles
  7. Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats
  8. Pharmacokinetic assessment of rifampicin and des-acetyl rifampicin in carbon tetrachloride induced liver injury model in Wistar rats
  9. Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
  10. Selenium prevented renal tissue damage in lipopolysaccharide-treated rats
  11. Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line
  12. Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6
  13. Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats
  14. Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
  15. The assessment of the mechanism of action of lauric acid in the context of oral cancer through integrative approach combining network pharmacology and molecular docking technology
  16. Therapeutic potential of flavonoids in ovalbumin induced asthma in mice model
  17. The effects of pioglitazone and rosiglitazone on liver function in hypothyroid rats
  18. Effect of ozone gas on viral kinetics and liver histopathology in hepatitis C patients
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