Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats

Onyinyechi Ruth Nwagwe; Stephen Adeniyi Adefegha; Ganiyu Oboh

doi:10.1515/jcim-2023-0101

Article

Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats

Onyinyechi Ruth Nwagwe , Stephen Adeniyi Adefegha and Ganiyu Oboh

Published/Copyright: November 17, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 21 Issue 1

Abstract

Objectives

This research work studied the phenolic composition of Pentaclethra macrophylla (PM), the inclusion of dietary supplementation of PM leaves on sexual functions and its connection to inhibit enzymes (arginase and phosphodiesterase-5) and nitric oxide level, linked to type 2 diabetes-induced erectile dysfunction in rats.

Methods

Gallic acid, chlorogenic and ellagic acids, Kaempferol, and epicatechin etc. was spotted with High performance liquid chromatography–diode array detector from PM extract. Twenty-five (25) rats were used for the study. Five rats were placed with basal diet; diets not supplemented with PM leaves (normal rat group) while twenty rats were made diabetic by feeding them with high fat diet for two weeks, prior to single injection with 35 mg/kg of streptozotocin (STZ). After checking with glucometer, experimental animals with blood glucose level >250 mg/dL were accepted as diabetic. The diabetic rats were subsequently divided into four groups of five rats each (n=5). The diabetic rats were placed on basal diet, or diets supplemented with PM leaves (10 % or 5 % inclusion) or sildenafil citrate (SC)

Results

The result revealed that PM supplemented diets caused significant (p<0.05) reduction in blood glucose level, and augmented erectile function by inhibiting arginase and PDE5 activities as well as enhancing nitric oxide level.

Conclusions

In conclusion, dietary inclusion of PM leaves could serve as a potent nutraceutical source in hyperglycemia induced erectile dysfunction management.

Keywords: Pentaclethra macrophylla ; sexual functions; nitric oxide; PDE5; diabetes

Corresponding authors: Onyinyechi Ruth Nwagwe, Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Laboratory, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria; and Department of Biochemistry, Federal University Oye Ekiti, P.M.B. 343, Oye, Nigeria, E-mail: onyinyechi.nwagwe@fuoye.edu.ng; and Ganiyu Oboh, Department of Biochemistry, Functional Foods, Nutraceuticals and Phytomedicine Laboratory, Federal University of Technology, P.M.B. 704, Akure 340001, Nigeria, E-mail: goboh@futa.edu.ng

Acknowledgments

Authors appreciate the effort of other members of the laboratory for the assiatance and support during the experiment.

Research ethics: The local Institutional Review Board deemed the study exempt from review.
Informed consent: Not applicable.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no competing interests.
Research funding: None declared.

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Received: 2023-04-30

Accepted: 2023-10-14

Published Online: 2023-11-17

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Articles in the same Issue

https://doi.org/10.1515/jcim-2023-0101

Keywords for this article

Pentaclethra macrophylla; sexual functions; nitric oxide; PDE5; diabetes