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Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line

  • Remya Jayakumar , Manoj Kumar Dash EMAIL logo , Saumya Gulati , Akanksha Pandey , Surendra Kumar Trigun and Namrata Joshi
Published/Copyright: November 30, 2023
Journal of Complementary and Integrative Medicine
From the journal Journal of Complementary and Integrative Medicine Volume 21 Issue 1

Abstract

Objectives

The invasive screening methods and the late stage diagnosis of colorectal carcinoma (CRC) are contributing for the devastative prognosis. The gradual shift of the disease pattern among younger generations requires the implementation of phytochemicals and traditional medicines. Arkeshwara rasa (AR) is a herb–mineral combination of Tamra bhasma/incinerated copper ashes and Dwigun Kajjali/mercury sulphide levigated with Calotropis procera leaf juice, Plumbago zeylanica root decoction and the decoction of three myrobalans (Terminalia chebula, Terminalia bellerica, Emblica Officinalis decoction)/Triphala decoction.

Methods

The SW-480 cell line was checked for the cytotoxicity and the cell viability criteria with MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) assay. The acridine orange/ethidium bromide (AO/EtBr) assay revealed the depth of apoptosis affected cells in the fluorescent images. The FTIR analysis exhibited the graphical spectrum of functional groups within the compound AR.

Results

The IC50 from the 10−7 to 10−3 concentrations against SW-480 cells was 40.4 μg/mL. The staining of AO/EtBr was performed to visualize live and dead cells and it is evident from the result that number of apoptotic cells increases at increasing concentration of AR. The single bond with stretch vibrations of O–H and N–H are more concentrated in the 2,500–3,200 cm−1 and 3,700–4,000 cm−1 of the spectra whereas, the finger print region carries the O–H and S=O type peaks.

Conclusions

The AR shows strong cyto-toxicity against the SW-480 cells by inducing apoptosis. It also modulates cellular metabolism with the involvement of functional groups which antagonizes the strong acids. Moreover, these effects need to be analyzed further based in the in vivo and various in vitro models.

Keywords: Arkeshwara rasa ; colorectal carcinoma; functional groups; FTIR; SW-480; MTT assay
Corresponding author: Dr. Manoj Kumar Dash, Associate Professor, Department of Rasa Shastra and Bhaishajya Kalpana, Government Ayurveda College, Raipur, Chhattisgarh, India, E-mail:

Acknowledgments

If applicable.

  1. Research ethics: The local Institutional Review Board deemed the study exempt from review.

  2. Informed consent: Informed consent was obtained from all individuals included in this study.

  3. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  4. Competing interests: Authors state no conflict of interest.

  5. Research funding: None declared.

  6. Data availability: Not applicable.

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Received: 2023-08-04
Accepted: 2023-11-01
Published Online: 2023-11-30

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2023-0221
Keywords for this article
Arkeshwara rasa; colorectal carcinoma; functional groups; FTIR; SW-480; MTT assay

Articles in the same Issue

  1. Frontmatter
  2. Reviews
  3. Markhamia lutea leaves aqueous and ethanolic extract with curative anti-inflammatory activity attenuates paclitaxel toxicity in rat’s intestine
  4. Does hydrotherapy influence plasma glucose levels in type 2 diabetes? – A scoping review
  5. Comparative effectiveness is the common denominator in health services research: experimental effects are promising, real-world effects are compelling
  6. Research Articles
  7. Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats
  8. Pharmacokinetic assessment of rifampicin and des-acetyl rifampicin in carbon tetrachloride induced liver injury model in Wistar rats
  9. Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
  10. Selenium prevented renal tissue damage in lipopolysaccharide-treated rats
  11. Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line
  12. Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6
  13. Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats
  14. Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
  15. The assessment of the mechanism of action of lauric acid in the context of oral cancer through integrative approach combining network pharmacology and molecular docking technology
  16. Therapeutic potential of flavonoids in ovalbumin induced asthma in mice model
  17. The effects of pioglitazone and rosiglitazone on liver function in hypothyroid rats
  18. Effect of ozone gas on viral kinetics and liver histopathology in hepatitis C patients
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