Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
-
Mohd Musaib Bhat
,
Ansar Ahmad
Abstract
Objectives
In Unani medicine, a comprehensive treatment plan has been delineated to deal with febrile illnesses using herbal drugs along with modified dietetics, which stands as a promising area of research. The present study was aimed at evaluating the antipyretic activity of the HAE of Artemisia absinthium L. whole plant as a standalone and as an adjuvant with barley water in an animal model of pyrexia to validate the age-old Unani principle of the treatment.
Methods
The pyrexia was induced in all the groups except the plain control using Brewer’s yeast. Group II did not receive any treatment, while group III received crocin, group IV received HAE of A. absinthium, group V administered Ma al-Sha’ir, and group VI was treated with the HAE of A. absinthium along with Ma al-Sha’ir by oral route. The rectal temperature of each rat was recorded at ‘0’ h, 30 min, 60 min, and 180 min.
Results
The mean rectal temperature of group III went down from 101.82±0.20 °F to 100.4±0.57 °F over the period of (0–180) minutes, whereas the mean temperature in group IV went down from 102.45±0.60 °F to 100.14±0.57 °F. The mean rectal temperature of group V decreased from 100.62±0.11 °F to 99.55±0.51 °F, while the mean rectal temperature of group VI went down from 101.95±0.1 °F to 97.7±0.11 °F.
Conclusions
It is concluded that the HAE of A. absinthium L. as a standalone and along with Ma al Sha’ir showed excellent antipyretic activity as compared to the standard drug in an animal model.
Acknowledgments
We are thankful to the Director General, Central Council for Research in Unani Medicine, New Delhi, Ministry of Ayush, Govt. of India for providing all the facilities required in this study.
-
Research ethics: The experimental protocol was approved by the Institutional Animal Ethics Committee (IAEC) of the Regional Research Institute of Unani Medicine, Srinagar, India vide Reg. No. 927/ GO/ Re/ S/ 2006/CPCSEA.
-
Informed consent: Not applicable.
-
Author contributions: Mohd Musaib Bhat and Athar Parvez Ansari: Designed protocol, carried out the whole study, collected data and compiled the whole manuscript. Ansar Ahmad: Critically reviewed the manuscript. Ifra Qayoom and Bazilah Majeed Reshi: Assisted the pre-clinical trial.
-
Competing interests: The authors state no conflict of interest.
-
Research funding: This work is part of the post graduate research programme of Regional Research Institute of Unani Medicine, Srinagar, CCRUM, New Delhi, Ministry of Ayush, Govt. of India. All the required facilities for this study were provided by the Central Council for Research in Unani Medicine, New Delhi.
-
Data availability: All data generated or analysed during this study are included in this published article.
References
1. Sullivan, JE, Farrar, HC. Fever and antipyretic use in children. Pediatrics 2011;127:580–7. https://doi.org/10.1542/peds.2010-3852.Search in Google Scholar PubMed
2. Kiekkas, P, Aretha, D, Bakalis, N, Karpouhtsi, I, Marneras, C, Baltopoulos, GI. Fever effects and treatment in critical care: literature review. Aust Crit Care 2013;26:130–5. https://doi.org/10.1016/j.aucc.2012.10.004.Search in Google Scholar PubMed
3. Gensini, GF, Conti, AA. The evolution of the concept of fever in the history of medicine: from pathological picture per se to clinical epiphenomenon. J Infect 2004;49:85–7. https://doi.org/10.1016/j.jinf.2003.11.003.Search in Google Scholar PubMed
4. Kushidayati, L. Ibn al-Jazzar on fevers: a critical edition of Zad al-Musafir wa-Qut al-Hadir by Gerrit Boss. Qudus Int J Islamic Stud 2014;2:183–97.Search in Google Scholar
5. Joel, GH, Lee, EL, Alfred, GG. Analgesic, antipyretic and anti-inflammatory agents. In: Goodman and Gilman’s: the pharmacological basis of therapeutics, 13th ed. USA: McGraw-Hill, Medical Publishing Division; 2018:687–93 pp.Search in Google Scholar
6. Roberts, E, Nunes, VD, Buckner, S, Latchem, S, Constanti, M, Miller, PD, et al.. Paracetamol: not as safe as we thought? A systematic literature review of observational studies. Ann Rheum Dis 2016;75:552–9. https://doi.org/10.1136/annrheumdis-2014-206914.Search in Google Scholar PubMed PubMed Central
7. Kelm, MA, Nair, MG, Strasburg, GM, DeWitt, DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum L. Phytomedicine 2000;7:7–13. https://doi.org/10.1016/S0944-7113(00)80015-X.Search in Google Scholar PubMed
8. Lisa, SR, Islam, MK, Qais, N. Plants and plant constituents with analgesic and anti-inflammatory activities. Dhaka Univ J Pharm Sci 2020;19:207–24. https://doi.org/10.3329/dujps.v19i2.50638.Search in Google Scholar
9. Zohr, I. Kitab al-Taisir (Urdu translation by CCRUM). New Delhi: Central Council for Research in Unani Medicine, Dept. of AYUSH, Ministry of H & FW, Govt. of India; 1984:339–2 pp.Search in Google Scholar
10. Khan, MA. Muheet-i-Azam (Urdu translation by CCRUM). New Delhi: Central Council for Research in Unani Medicine, Dept. of AYUSH, Ministry of H & FW, Govt. of India; 2012, vol 1 & 2:232–5, 359–3 pp.Search in Google Scholar
11. Ghani, N. Khazain al-Adwiya. New Delhi: Idara Kitab al-Shifa; 2011:173–4, 245–6 pp.Search in Google Scholar
12. Nadkarni, KM. Indian Materia Medica, 3rd ed. Bombay: Popular Prakashan Pvt. Ltd.; 2013:141–2, 1108–13 pp.Search in Google Scholar
13. Khattak, S, Gilama, SN, Ikram, M. Antipyretic studies on some indigenous Pakistani medicinal plants. J Ethnopharmacol 1985;14:45–1.10.1016/0378-8741(85)90027-3Search in Google Scholar PubMed
14. Razi, AMZ. Kitab al-Hawi, (Urdu translation by CCRUM), vol 7. New Delhi: Central Council for Research in Unani Medicine, Dept. of AYUSH, Ministry of H & FW, Govt. of India; 2000:47–50 pp.Search in Google Scholar
15. Anonymous. The Unani Pharmacopoeia of India. New Delhi: Dept. of AYUSH, Ministry of H & FW, Govt. of India; 2007, vol 2:3–4 pp.Search in Google Scholar
16. Ansari, AP, Ahmed, NZ, Wadud, A, Arif, M, Khanday, S. Ilaj bil Ghiza (Dietotherapy): a core mode of Unani treatment. J Adv Res Pharm Sci Pharmacol Interv 2018;2:27–5.Search in Google Scholar
17. Anonymous. The Wealth of India – a dictionary of Indian raw materials & industrial products, 3rd ed. New Delhi: Publication & Information Directorate, CSIR; 2005:295–3 pp.Search in Google Scholar
18. Kabiruddin, M. Makhzan al-Mufradat. New Delhi: Aijaz Publishing House; 2005:225–6 pp.Search in Google Scholar
19. Baghdadi, IH. Kitab al-Mukhtarat fi al-Tib (Urdu translation by CCRUM). New Delhi: Central Council for Research in Unani Medicine, Dept. of AYUSH, Ministry of H & FW, Govt. of India; 2005, vol 1. 216–8 pp.Search in Google Scholar
20. Kabiruddin, M. Humiyat-i-Qanun. New Delhi: Aijaz Publishing House; 2007:305 p.Search in Google Scholar
21. Kabiruddin, M. Al-Qarabadeen. New Delhi: Central Council for Research in Unani Medicine, Dept. of AYUSH, Ministry of H & FW, Govt. of India. Central Council for Research in Unani Medicine; 2006:1020–2 pp.Search in Google Scholar
22. Taraghdari, SB, Nematy, M, Mazidi, M, Kamgar, M, Soukhtanloo, M, Hosseini, M, et al.. The effect of hydro-alcoholic extract of Artemisia absinthium on appetite in male rats. Avicenna J Phytomed 2015;5:78–3.Search in Google Scholar
23. Arzani, A. Qarabadeen-i-Qadri. New Delhi: Aijaz Publishing House; 1998:450–3 pp.Search in Google Scholar
24. Vogel, GH, Vogel, WH. Drug discovery and evaluation, pharmacological assay. Germany: Springer; 2002:772 p.10.1007/3-540-29837-1Search in Google Scholar
25. Susrata, Kunja L, Bhishagratna. The Susruta Samhita- an English translation based on original texts. Calcutta: Uttara-Tantra; 1907:169–2 pp.Search in Google Scholar
26. Mackowiak, PA. Brief history of antipyretic therapy. Clin Inf Dis 2000;31:154–6. https://doi.org/10.1086/317510.Search in Google Scholar PubMed
27. Beauhaire, J, Fourrey, JL, Guittet, E. Structure of absintholide, a new guaianolide dimer of Artemisia absinthium L. Tetrahedron Lett 1984;25:2751, https://doi.org/10.1016/s0040-4039(01)81281-8.Search in Google Scholar
28. Teotino, UM, Friz, LP, Gendivi, A, Bella, DD. The derivatives of 2, 3-dihydro-4H-1,3benzoxazin-4-one. Synthesis and pharmacological properties. J Med Chem 1963;6:248–50, https://doi.org/10.1021/jm00339a007.Search in Google Scholar PubMed
29. Ahamad, J, Mir, SR, Amin, S. A pharmacognostic review on Artemisia absinthium. Int Res J Pharm 2019;10:25–1, https://doi.org/10.7897/2230-8407.10015.Search in Google Scholar
30. Omwamba, M, Hu, Q. Antioxidant activity in barley (Hordeum vulgare L.) grains roasted in a microwave oven under conditions optimized using response surface methodology. J Food Sci 2010;75:66–3. https://doi.org/10.1111/j.1750-3841.2009.01426.x.Search in Google Scholar PubMed
31. Shah, JG, Patel, BG, Patel, SB, Patel, RK. Antiurolithiatic and antioxidant activity of Hordeum vulgare seeds on ethylene glycol-induced urolithiasis in rats. Indian J Pharmacol 2012;44:672–7. https://doi.org/10.4103/0253-7613.103237.Search in Google Scholar PubMed PubMed Central
© 2023 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- Markhamia lutea leaves aqueous and ethanolic extract with curative anti-inflammatory activity attenuates paclitaxel toxicity in rat’s intestine
- Does hydrotherapy influence plasma glucose levels in type 2 diabetes? – A scoping review
- Comparative effectiveness is the common denominator in health services research: experimental effects are promising, real-world effects are compelling
- Research Articles
- Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats
- Pharmacokinetic assessment of rifampicin and des-acetyl rifampicin in carbon tetrachloride induced liver injury model in Wistar rats
- Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
- Selenium prevented renal tissue damage in lipopolysaccharide-treated rats
- Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line
- Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6
- Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats
- Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
- The assessment of the mechanism of action of lauric acid in the context of oral cancer through integrative approach combining network pharmacology and molecular docking technology
- Therapeutic potential of flavonoids in ovalbumin induced asthma in mice model
- The effects of pioglitazone and rosiglitazone on liver function in hypothyroid rats
- Effect of ozone gas on viral kinetics and liver histopathology in hepatitis C patients
Articles in the same Issue
- Frontmatter
- Reviews
- Markhamia lutea leaves aqueous and ethanolic extract with curative anti-inflammatory activity attenuates paclitaxel toxicity in rat’s intestine
- Does hydrotherapy influence plasma glucose levels in type 2 diabetes? – A scoping review
- Comparative effectiveness is the common denominator in health services research: experimental effects are promising, real-world effects are compelling
- Research Articles
- Hydroethanolic extract of Piliostigma thonningii leaves extenuates the severity of diarrhoea in female Wistar rats
- Pharmacokinetic assessment of rifampicin and des-acetyl rifampicin in carbon tetrachloride induced liver injury model in Wistar rats
- Antipyretic activity of the hydro-alcoholic extract of Artemisia absinthium L. as a standalone and as an adjuvant with barley water against yeast-induced pyrexia in albino Wistar rats
- Selenium prevented renal tissue damage in lipopolysaccharide-treated rats
- Preliminary data on cytotoxicity and functional group assessment of a herb–mineral combination against colorectal carcinoma cell line
- Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6
- Pentaclethra macrophylla leaves reduce blood glucose level, improve sexual behavior and modulate critical enzymes associated with diabetes-erectile dysfunction morbidity in rats
- Anti-melanoma and antioxidant properties of the methanol extract from the leaves of Phragmenthera capitata (Spreng.) Balle and Globimetula braunii (Engl.) Van Tiegh
- The assessment of the mechanism of action of lauric acid in the context of oral cancer through integrative approach combining network pharmacology and molecular docking technology
- Therapeutic potential of flavonoids in ovalbumin induced asthma in mice model
- The effects of pioglitazone and rosiglitazone on liver function in hypothyroid rats
- Effect of ozone gas on viral kinetics and liver histopathology in hepatitis C patients
