Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6

Rizki Sandhi Titisari; Elisa Herawati; Okid Parama Astirin

doi:10.1515/jcim-2023-0209

Article

Oral intake of collagen hydrolysate from mackerel scad (Decapterus macarellus) attenuates skin photoaging by suppressing the UVB-induced expression of MMP-1 and IL-6

Rizki Sandhi Titisari , Elisa Herawati and Okid Parama Astirin

Published/Copyright: November 15, 2023

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From the journal Journal of Complementary and Integrative Medicine Volume 21 Issue 1

Abstract

Objectives

Excessive skin exposure to UVB radiation can induce photoaging caused by an imbalance in oxidative stress and inflammatory responses, damaging the skin’s structure and surface layer. A previous study revealed that collagen hydrolisate extracted from the skin of mackarel scads (Decapterus macarellus) had antiaging properties that were tested in vitro, which serves as a foundation for a subsequent study of its use in vivo. This study aimed at investigating the repair effect of the mackerel scad’s skin collagen hydrolysate (MSS-CH) in photoaging conditions in a mouse model.

Methods

MSS-CH was given orally in mice model of skin photoaging under chronic exposure to UVB irradiation for 12 weeks. Morphological and histological changes on the skin were evaluated using SEM and HE staining, along with the measurement of the expression of matrix metalloproteinases (MMP-1) and cytokine pro-inflammatory interleukin 6 (IL-6) using ELISA.

Results

MSS-CH inhibits the occurrence of epidermal thickening and damage to the dermal layer of the skin. As a result, it restores the epidermis’ barrier function and reduces surface damage caused by photoaging. The skin of the MSS-CH treated group exhibited improved physical appearance with reduced fine lines, wrinkles, and enhanced smoothness. Additionally, administering MSS-CH to the mice groups reduced the expression of MMP-1 and IL-6 in UVB-exposed skin.

Conclusions

Altogether, this in vivo study demonstrates the photoaging-protective properties of CH-MSS, aligning with previous in vitro data. Thus, MSS-CH emerges as a strong candidate for use as an ingredient in nutraceuticals and biocosmetics.

Keywords: photoaging; collagen hydrolysate; mackerel scad skin; MMP-1; IL-6

Corresponding author: Elisa Herawati, PhD Graduate Program of Bioscience, Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret, Jl. Ir. Sutami 36A, Surakarta, Central Java 57126, Indonesia, E-mail: elisahera@staff.uns.ac.id

Funding source: Research Grant from Indonesian Ministry of Education, Culture, Research, and Technology, Indonesia

Award Identifier / Grant number: 673.1/UN27.22/PT.01.03/2022

Acknowledgments

We thank Dr. Shanti Listyawati for assistance with the handling of laboratory animals and the comments that greatly improved the manuscript.

Research ethics: The research related to animal’s use has complied with all the relevant institutional policies of Research Ethical Committee of Faculty of Medicine of Universitas Sebelas Maret for the care and use of animals (Number: 03/UN27.06.11/KEP/EC/2023).
Informed consent: Not applicable.
Author contributions: Rizki Sandhi Titisari: Investigation, Formal analysis, Writing – original draft. Elisa Herawati: Conceptualization, Investigation, Formal analysis, Project administration, Writing – review & editing. Okid Parama Astirin: Supervision, Writing – review.
Competing interests: Authors state no conflict of interest.
Research funding: This work was funded by Indonesian Ministry of Education, Culture, Research, and Technology, Indonesia (Grant Number 673.1/UN27.22/PT.01.03/2022).

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Received: 2023-07-27

Accepted: 2023-10-23

Published Online: 2023-11-15

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Articles in the same Issue

https://doi.org/10.1515/jcim-2023-0209

Keywords for this article

photoaging; collagen hydrolysate; mackerel scad skin; MMP-1; IL-6