Startseite Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella
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Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella

  • Sabrina Ferreira dos Santos Liberato ORCID logo , Mariana Raquel da Cruz Vegian ORCID logo , Amjad Abu Hasna ORCID logo EMAIL logo , Janaína Araújo de Alvarenga , Juliana Guimarães dos Santos , Ítalo Rigotti Pereira Tini , Isabela Amêndola , Juliana Campos Junqueira ORCID logo und Luciane Dias de Oliveira ORCID logo
Veröffentlicht/Copyright: 16. Juli 2021
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 19 Heft 4

Abstract

Objectives

This study aimed to evaluate the antibiofilm activity and toxicity of the glycolic extract of Persea americana “P. americana” over multidrug-resistant strains of Acinetobacter baumannii “A. baumannii” as alternative therapy to be investigated.

Methods

A bacterial inoculum of each bacterial strain (4a, 5a, 9a, 12a, ATCC 19606) of A. baumannii was prepared and adjusted by the spectrophotometer. The microdilution broth method was performed to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). P. americana glycolic extracts were obtained of the tree stalk and leaves. The biofilm viability was tested by MTT assay after 5 min exposure. The toxicity of the extracts was tested by invertebrate model Galleria mellonella. The data were analyzed by ANOVA, Tukey test and log-rank method (α=0.05).

Results

The extract showed an inhibitory and bactericidal action over all the tested strains with the lowest MIC value observed for the reference strain (3.12 mg/mL). The extract did not demonstrate toxicity in any of the tested concentrations (12.5, 25 and 50 mg/mL) in Galleria mellonella larvae, with a survival percentage above 80% after 168 h.

Conclusions

The glycolic extract of P. americana has microbicidal and antibiofilm activity on multidrug-resistant clinical strains of A. baumannii and showed low toxicity for the invertebrate model G. mellonella.

Keywords: Acinetobacter baumannii ; antibacterial agents; Persea americana ; phytotherapy
Corresponding author: Amjad Abu Hasna, Department of Restorative Dentistry, Endodontics division, Institute of Science and Technology, São Paulo State University – UNESP, Av. Eng. Francisco José Longo, 777 – Jardim Sao Dimas, São José dos Campos – SP, 12245-000 São Paulo, Brazil, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: The local Institutional Review Board deemed the study exempt from review.

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Received: 2021-02-03
Accepted: 2021-07-05
Published Online: 2021-07-16

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. A preliminary systematic review and meta-analysis on the effects of heart rate variability biofeedback on heart rate variability and respiration of athletes
  4. The potential positive epigenetic effects of various mind-body therapies (MBTs): a narrative review
  5. The effect of Chlorella vulgaris on obesity related metabolic disorders: a systematic review of randomized controlled trials
  6. Biological and medicinal application of Cucumis sativus Linn. – review of current status with future possibilities
  7. The effect of the herbal medicine on severity of cyclic mastalgia: a systematic review and meta-analysis
  8. Research Articles
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  11. Fermented maize slurry (Ogi) and its supernatant (Omidun) mitigate elevated intraocular pressure by modulating BDNF expression and glial plasticity in the retina-gut axis of glaucomatous rats
  12. Levetiracetam exposure during prenatal and postnatal period induces cognitive decline in rat offsprings, not completely prevented by Bacopa monnieri
  13. Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella
  14. Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats
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  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
  20. Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies
  21. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats
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  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
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  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
https://doi.org/10.1515/jcim-2021-0051
Schlagwörter für diesen Artikel
Acinetobacter baumannii; antibacterial agents; Persea americana; phytotherapy

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. A preliminary systematic review and meta-analysis on the effects of heart rate variability biofeedback on heart rate variability and respiration of athletes
  4. The potential positive epigenetic effects of various mind-body therapies (MBTs): a narrative review
  5. The effect of Chlorella vulgaris on obesity related metabolic disorders: a systematic review of randomized controlled trials
  6. Biological and medicinal application of Cucumis sativus Linn. – review of current status with future possibilities
  7. The effect of the herbal medicine on severity of cyclic mastalgia: a systematic review and meta-analysis
  8. Research Articles
  9. Chemical compositions, antibacterial, antifungal and cytotoxic effects of Alhagi mannifera five extracts
  10. Anticonvulsive and anti-epileptogenesis effects of Echinacea purpurea root extract, an involvement of CB2 receptor
  11. Fermented maize slurry (Ogi) and its supernatant (Omidun) mitigate elevated intraocular pressure by modulating BDNF expression and glial plasticity in the retina-gut axis of glaucomatous rats
  12. Levetiracetam exposure during prenatal and postnatal period induces cognitive decline in rat offsprings, not completely prevented by Bacopa monnieri
  13. Antibiofilm action of Persea americana glycolic extract over Acinetobacter baumannii and absence of toxicity in Galleria mellonella
  14. Validation of Unani concept of Abadāl-i-Adwiya (drug substitution) by physicochemical standardization and hepatoprotective activity of Aristolochia rotunda Linn. and its substitute Curcuma Zedoaria Rosc. in albino Wistar rats
  15. Cinnamon oil as a co-chemotherapy agent through inhibition of cell migration and MMP-9 expression on 4T1 cells
  16. Assessment of biochemical changes in normal and diabetic rats treated by phenolic enriched extracts of Juglans regia L. barks
  17. Influence of Clerodendrum volubile leaf extract on doxorubicin-induced toxicity and inhibition of carbonyl reductase mediated metabolism
  18. Quantification of anacardic acid, the toxic component in raw and purified samples of Semecarpus anacardium L. by Siddha purification processes
  19. Molecular docking and molecular dynamics approach to identify potential compounds in Huperzia squarrosa for treating Alzheimer’s disease
  20. Evaluation of ethanol extracts from three species of Artocarpus as natural gastroprotective agents: in vivo and histopathological studies
  21. Attenuation of cisplatin induced myelosuppression by methanol extract of Cedrus deodara in Wistar rats
  22. Acute and sub-acute toxicity assessment of the standardized extract of Sanguisorba minor in vivo
  23. Efficacy of lettuce seed syrup on insomnia in patients with breast cancer: a pilot double blind randomized placebo controlled clinical trial
  24. The effect of aromatherapy with rose essential oil on apparent anxiety in patients with myocardial infarction
  25. Effect of Jyoti-Trataka on intraocular pressure, autonomic control, and blood glucose in diabetic patients with high-tension primary open-angle glaucoma: a randomized-controlled trial
  26. Efficacy of Ḥammām-i-yābis (dry bath) in metabolic syndrome: a single arm, open-labelled clinical trial
  27. Effect of Ḥijāma (wet cupping), Dalk (massage) and Bukhūr (medicated steam) in amelioration of Waja al-Zahr (non-specific low back pain) – an open prospective clinical trial
  28. Effect of yoga on cardiovascular functions and psychological aspects of people on public service-related work: an exploratory study
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