Startseite Evaluation of Moroccan microalgae: Spirulina platensis as a potential source of natural antioxidants
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Evaluation of Moroccan microalgae: Spirulina platensis as a potential source of natural antioxidants

  • Touria Ould Bellahcen EMAIL logo , Abderrahmane AAmiri , Ikram Touam , Fouzia Hmimid , Abdelaziz El Amrani , Abdelmjid Cherif und Mounia Cherki
Veröffentlicht/Copyright: 23. September 2020
Journal of Complementary and Integrative Medicine
Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 17 Heft 3

Abstract

Background

Ethanolic, aqueous and lipidic extracts of Spirulina platensis were evaluated for their bioactive substances (polyphenols and fatty acids) and antioxidant activities using two different assays diphenyl-1-picrylhydrazyl (DPPH) and azino-bis (ethylbenzthiazoline-6- sulfonicacid (ABTS)).

Methods

The phenolic compounds amounts were determined by colorimetric assays and were analyzed by high-performance and liquid chromatography (HPLC) method. The identification of phenolic compounds in the extract was accomplished by comparison of their retention times with those of pure standards. The chemical composition of fatty acids in lipidic extract was determined by GC/FIDs and GC/MS method.

Results

The obtained results revealed that using DPPH, the ethanolic extract recorded the highest activity with (IC50=449 μg/mL ± 83). It was characterized with a high levels of phenolic and flavonoid content 0.33 ± 0.01 mg GAE/g dw and 0.21 ± 0.01 mg quercetin/g dw, respectively. The ethanolic extract showed the presence of caffeic acid, syringic acid, ferulic acid, p-coumaric acid, chlorogenic acid, kaempferol, quercetin and apigenin. Those entire phenolic compounds seem participle synergistically to the pronounced higher activity. To the best of our knowledge, the apigenin (4′, 5, 7,-trihydroxyflavone), a natural compound which is famously known by its potent antiradical activity, was identified for the first time in Moroccan S. platensis only in the ethanolic extract. A high positive correlation (r=0.895) between the antioxidant activity and the chemical composition of ethanolic extract was observed. For ABTS, lipidic extract showed the highest activity with (IC50=740 μg/mL ± 12). A total of 15 fatty acids compounds, amounting 89.73% of the extract were identified. γ-linolenic acid (GLA) (39.02%) and α-linolenic acid (ALA) (13.85%) were the major component and contribute greatly to the antioxidant activity observed. The lipidic extract has a high content of unsaturated fatty acids (70.95%) which are often exhibited very high antioxidant activity since they have two or more double bonds and chain lengths. The aqueous extract either with DPPH or ABTS methods, recorded low antioxidant activity. Its correlation was r=0.499, lower than found in the ethanolic extract.

Conclusions

These results showed that Spirulina platensis could be considered as a valuable source of fatty acids and phenolics with potent antioxidant activity. It may be employed in the manufacture of pharmaceutical drugs as an alternative source of natural antioxidants.

Keywords: ABTS; antioxidant; apigenin; DPPH; Spirulina platensis; γ-linolenic acid

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: On behalf all coauthors, I confirm that we not have conflict of interest with any reviewers or any financial and personal relationships with other people or organizations that could inappropriately influence our work.

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Received: 2019-05-11
Accepted: 2019-09-16
Published Online: 2020-09-23

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jcim-2019-0036
Schlagwörter für diesen Artikel
ABTS; antioxidant; apigenin; DPPH; Spirulina platensis; γ-linolenic acid

Artikel in diesem Heft

  1. Reviews
  2. Hammam-i-Bukhari (Turkish bath): A promising regimenal mode of Unani treatment
  3. Assessment the studies on the concept of Mizaj (temperament) in Persian Medicine
  4. Phytotherapy in endometriosis: an up-to-date review
  5. Research Articles
  6. Preclinical
  7. Exploring the phytochemical and nutraceutical potentials of dasapatrachurnam
  8. Evaluation of Moroccan microalgae: Spirulina platensis as a potential source of natural antioxidants
  9. The Metabolomic study of Calotropis procera Ait. from Burkina Faso, based on chemical functional groups profiling using FTIR
  10. Hepatoprotective and antioxidant effect of Adiantum lunulatum Burm. F. leaf in alcohol-induced rat model
  11. Protective effect of Matricaria chamomilla extract against 1,2-dimethylhydrazine-induced colorectal cancer in mice
  12. Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster
  13. Clinical
  14. Factors determining choice of complementary and alternative medicine in acute and chronic diseases
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  16. A randomized trial of the effect of peer education on the sexual quality of life in patients with myocardial infarction
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  19. Compare the effect of aromatherapy using lavender and Damask rose essential oils on the level of anxiety and severity of pain following C-section: A double-blinded randomized clinical trial
  20. Effectiveness of yogic visual concentration (Trataka) on cognitive performance and anxiety among adolescents
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