Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury
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Mohammadreza Gholami
Abstract
Introduction
The aim of the present study was to survey the protective effect of pretreatment with Persian honey on amelioration of side effects of chemotherapy and ischemia/reperfusion induced testicular injury.
Materials and methods
Forty adult’s male wistar rats were divided into four groups of ischemia-reperfusion (IR), honey + ischemia-reperfusion (HIR), Busulfan (B) and Busulfan intraperitoneally+ honey (BH). The seminiferous tubules were rated for their modified spermatogenesis index (SI) by Johnsons score. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed using the in-situ cell death detection kit. Total serum concentration of Follicle-stimulating hormone (FSH) , Luteinizing hormone (LH) and testosterone was measured using ELISA. All data were expressed as mean ± SD and significance was set at p≤0.05.
Results
Honey improved SI in the HIR and BH groups and serum levels of FSH and LH in the BH and HIR groups (p<0.001). Also, serum levels of testosterone were significantly higher in BH and HIR groups. But, apoptotic cells in IR and B groups significantly increased (p<0.001), while in HIR and BH groups, the number of apoptotic cells decreased and the positive cells of TUNEL (TdT-mediated dUTP-X nick end labelling) staining were detected in spermatocytes and spermatid.
Discussion
Pretreatment with honey protect testis against chemotherapy and testicular IR injury, increase FSH and LH and testosterone and decrease the cellular damage and apoptosis. Honey can decrease the side effects of chemotherapy on reproductive system and prevent sterility.
Acknowledgements
This study approved in the Lorestan University of Medical Sciences. Special thanks to Lorestan University of Medical Sciences for the financial support, Khorramabad, Iran. The authors thank the head and staff of Razi Herbal Medicines Research Center of Lorestan Medical University.
Author contributions: The authors contributed equally in the study concept and design, acquisition of data, statistical analysis, interpretation of data, and drafting of the manuscript.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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© 2018 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Pre-clinical Studies
- Acute and subacute toxicity of aqueous extract of the tuber of Kedrostis africana (L.) Cogn in Wistar rats
- Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury
- Chemical composition and antioxidant activity of phenolic compounds and essential oils from Calamintha nepeta L.
- Analgesic, anti-inflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiacee) in rats
- Testicular and spermatotoxic potential of methanol extract of red cultivar Allium cepa in rats
- Clinical Studies
- Premature ovarian failure of autoimmune etiology in 46XX patients: is there a hope?
- African crocus (Curculigo pilosa) and wonderful kola (Buchholzia coriacea) seeds modulate critical enzymes relevant to erectile dysfunction and oxidative stress
- Effect of foot reflexology and slow stroke back massage on the severity of fatigue in patients undergoing hemodialysis: A semi-experimental study
- Effects of Spiritist “passe” (Spiritual healing) on stress hormone, pain, physiological parameters and length of stay in preterm newborns: a randomized, double-blind controlled trial
- Short Communication
- A pilot-study of hypnotherapy as complementary treatment for pain in chronic pancreatitis
- Neuroprotective effect of curcumin nanoparticles against rat model of status epilepticus induced by pilocarpine
- Yoga practice to improve sleep quality and body composition parameters of obese male - a randomized controlled trial
- Association between a guided meditation practice, sleep and psychological well-being in type 2 diabetes mellitus patients
- Case study
- Effect of yoga and naturopathy on liver, renal and cardiorespiratory functions of a patient with hepatic cirrhosis with portal hypertension and ascites: a case report
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Artikel in diesem Heft
- Pre-clinical Studies
- Acute and subacute toxicity of aqueous extract of the tuber of Kedrostis africana (L.) Cogn in Wistar rats
- Protective effects of persian honey, Apis Mellifera Meda Skorikov on side effects of chemotherapy and ischemia/reperfusion induced testicular injury
- Chemical composition and antioxidant activity of phenolic compounds and essential oils from Calamintha nepeta L.
- Analgesic, anti-inflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiacee) in rats
- Testicular and spermatotoxic potential of methanol extract of red cultivar Allium cepa in rats
- Clinical Studies
- Premature ovarian failure of autoimmune etiology in 46XX patients: is there a hope?
- African crocus (Curculigo pilosa) and wonderful kola (Buchholzia coriacea) seeds modulate critical enzymes relevant to erectile dysfunction and oxidative stress
- Effect of foot reflexology and slow stroke back massage on the severity of fatigue in patients undergoing hemodialysis: A semi-experimental study
- Effects of Spiritist “passe” (Spiritual healing) on stress hormone, pain, physiological parameters and length of stay in preterm newborns: a randomized, double-blind controlled trial
- Short Communication
- A pilot-study of hypnotherapy as complementary treatment for pain in chronic pancreatitis
- Neuroprotective effect of curcumin nanoparticles against rat model of status epilepticus induced by pilocarpine
- Yoga practice to improve sleep quality and body composition parameters of obese male - a randomized controlled trial
- Association between a guided meditation practice, sleep and psychological well-being in type 2 diabetes mellitus patients
- Case study
- Effect of yoga and naturopathy on liver, renal and cardiorespiratory functions of a patient with hepatic cirrhosis with portal hypertension and ascites: a case report
- Review
- Phytochemistry, bioactivity: suggestion of Ceratonia siliqua L. as neurodegenerative disease therapy
- Abstracts
- 2018 INCAM Research Symposium: Complementary and Integrative Medicine Research in an Era of Skepticism