Home Medicine Analgesic, anti-inflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiacee) in rats
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Analgesic, anti-inflammatory and anti-arthritic properties of aqueous and methanolic stem bark extracts from Nauclea pobeguinii (Rubiacee) in rats

  • Mbiantcha Marius EMAIL logo , Tsafack Eric Gonzal , Ateufack Gilbert , Nana Yousseu William , Bomba Tatsinkou Francis Désiré , Djuichou Nguemnang Stephanie Flore , Mbankou Ngassam Sorelle and Wego Kamgaing Marius Tresor
Published/Copyright: May 23, 2018

Abstract

Background

Nauclea pobeguinii is a plant species found in the centre region of Cameroon. The stem bark of this plant is traditionally used to ease pain and cure inflammation.

Method

This study was undertaken to evaluate the effects of doses 150 and 300 mg/kg of the aqueous and methanolic stem bark extracts from Nauclea pobeguinii on acute pain, acute and chronic inflammation induced by formalin and arthritis induced by zymosan A in rats. Oxidative stress parameters such as catalase, malondialdehyde and nitric oxide were measured in rats subjected to chronic inflammation. The standard used was diclofenac at 5 mg/kg.

Results

Aqueous extract as well as methanolic extract of Nauclea pobeguinii led to a significant reduction in the second phase of formalin induced pain with 54.22 and 48.02% of inhibition percentage, respectively. The formalin-induced inflammatory oedema was reduced by both extracts, and this effect remains significant until the tenth day of treatment. Equally, extracts significantly increased the catalase activity and inhibited the production of malondialdehyde (MDA) in serum, brain and spinal cord and NO reduction only in serum. Both extracts significantly reduced the articular oedema induced by zymosan A for 6 h and for 5 days. Furthermore, the histological study of the articulations shows a non-altered synovial membrane and a small cartilage in all treated animals versus negative control group.

Conclusions

From these results, it can be concluded that pain, inflammation and arthritic healing activities of both stem bark extracts were expressed in rats and could conciliate the use of this vegetable by traditional African healers.

  1. Availability of data and materials: All data supporting our findings are adequately contained within the manuscript.

  2. Authors’ contributions: MM, TEG and AG designed the work. MM, TEG, DNSF, MNS and WKMT conducted the work, collected and analysed the data. MM, NYW, BTFD and AG drafted the manuscript and revised it critically. All authors agree to be accountable for all aspects of the work.

  3. Authors’ information: MM (PhD) is Senior Lecturer in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. BTFD (PhD) is researcher in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. TEG, NYW, DNSF, MNS, WKMT are PhD students in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon. AG is an Associate Professor in the Department of Animal Biology, Faculty of Science, University of Dschang, Cameroon.

  4. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  5. Research funding: None declared.

  6. Employment or leadership: None declared.

  7. Honorarium: None declared.

  8. Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2018-01-29
Accepted: 2018-04-14
Published Online: 2018-05-23

© 2018 Walter de Gruyter GmbH, Berlin/Boston

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