Startseite Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation
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Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation

  • Lorenzo A. Cingolani ORCID logo EMAIL logo , Agnes Thalhammer ORCID logo EMAIL logo , Fanny Jaudon ORCID logo , Jessica Muià und Gabriele Baj
Veröffentlicht/Copyright: 4. September 2023
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 404 Heft 10

Abstract

The distance between CaV2.1 voltage-gated Ca2+ channels and the Ca2+ sensor responsible for vesicle release at presynaptic terminals is critical for determining synaptic strength. Yet, the molecular mechanisms responsible for a loose coupling configuration of CaV2.1 in certain synapses or developmental periods and a tight one in others remain unknown. Here, we examine the nanoscale organization of two CaV2.1 splice isoforms (CaV2.1[EFa] and CaV2.1[EFb]) at presynaptic terminals by superresolution structured illumination microscopy. We find that CaV2.1[EFa] is more tightly co-localized with presynaptic markers than CaV2.1[EFb], suggesting that alternative splicing plays a crucial role in the synaptic organization of CaV2.1 channels.

Keywords: alternative splicing; CaV2.1; Munc13; presynaptic terminals; structural illumination microscopy; voltage-gated Ca2+ channels
Corresponding authors: Lorenzo A. Cingolani, Department of Life Sciences, University of Trieste, via Giorgieri 5, I-34127 Trieste, Italy; and Center for Synaptic Neuroscience and Technology (NSYN), Fondazione Istituto Italiano di Tecnologia (IIT), Largo Rosanna Benzi 10, I-16132 Genoa, Italy, E-mail: ; and Agnes Thalhammer, Department of Life Sciences, University of Trieste, via Giorgieri 5, I-34127 Trieste, Italy; and Centro Interdipartimentale di Microscopia Avanzata (CIMA), University of Trieste, via Fleming 31, I-34127 Trieste, Italy, E-mail:

Funding source: Fondazione Telethon

Award Identifier / Grant number: GGP19181

Acknowledgments

This work was supported by the Telethon foundation (proposal ID: GGP19181 to LAC).

  1. Research ethics: All animal procedures were in accordance with the guidelines for animal welfare used in scientific research of the Italian Government and the local governance.

  2. Author contributions: AT and LAC concieved the project. AT, FJ, GB and LAC designed experiments. AT, FJ and JM performed experiments, AT and FJ analysed data, AT, FJ and LAC prepared figures and wrote the paper. The authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: The authors state no conflict of interest.

  4. Research funding: Telethon foundation, proposal GGP19181.

  5. Data availability: The raw data can be obtained on request from the corresponding author.

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Supplementary Material

This article contains supplementary material (https://doi.org/10.1515/hsz-2023-0235).

Received: 2023-06-12
Accepted: 2023-08-01
Published Online: 2023-09-04
Published in Print: 2023-09-26

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2023-0235
Schlagwörter für diesen Artikel
alternative splicing; CaV2.1; Munc13; presynaptic terminals; structural illumination microscopy; voltage-gated Ca2+ channels

Artikel in diesem Heft

  1. Frontmatter
  2. Highlights in biochemistry Bochum 2022
  3. Highlights in biochemistry Bochum 2022
  4. Two are not enough: synthetic strategies and applications of unnatural base pairs
  5. The emerging role of ATP as a cosolute for biomolecular processes
  6. Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel
  7. Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation
  8. Rodent models for mood disorders – understanding molecular changes by investigating social behavior
  9. Why do certain cancer cells alter functionality and fuse?
  10. Research Articles/Short Communications
  11. Cell Biology and Signaling
  12. MicroRNA-101-3p inhibits nasopharyngeal carcinoma cell proliferation and cisplatin resistance through ZIC5 down-regulation by targeting SOX2
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