Abstract
Nucleic acid chemistry is a rapidly evolving field, and the need for novel nucleotide modifications and artificial nucleotide building blocks for diagnostic and therapeutic use, material science or for studying cellular processes continues unabated. This review focusses on the development and application of unnatural base pairs as part of an expanded genetic alphabet. Not only recent developments in “nature-like” artificial base pairs are presented, but also current synthetic methods to get access to C-glycosidic nucleotides. Wide-ranging viability in synthesis is a prerequisite for the successful use of unnatural base pairs in a broader spectrum and will be discussed.
Funding source: Boehringer Ingelheim Foundation
Award Identifier / Grant number: Plus 3 Grant
Funding source: Juergen Manchot Foundation
Award Identifier / Grant number: PhD stipend to P.K.W.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This work was supported by the Boehringer Ingelheim Foundation (Plus 3 Grant) and Juergen Manchot Foundation (PhD stipend to P.K.W.).
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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Articles in the same Issue
- Frontmatter
- Highlights in biochemistry Bochum 2022
- Highlights in biochemistry Bochum 2022
- Two are not enough: synthetic strategies and applications of unnatural base pairs
- The emerging role of ATP as a cosolute for biomolecular processes
- Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel
- Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation
- Rodent models for mood disorders – understanding molecular changes by investigating social behavior
- Why do certain cancer cells alter functionality and fuse?
- Research Articles/Short Communications
- Cell Biology and Signaling
- MicroRNA-101-3p inhibits nasopharyngeal carcinoma cell proliferation and cisplatin resistance through ZIC5 down-regulation by targeting SOX2
Articles in the same Issue
- Frontmatter
- Highlights in biochemistry Bochum 2022
- Highlights in biochemistry Bochum 2022
- Two are not enough: synthetic strategies and applications of unnatural base pairs
- The emerging role of ATP as a cosolute for biomolecular processes
- Intracellular spatially-targeted chemical chaperones increase native state stability of mutant SOD1 barrel
- Nanoscale organization of CaV2.1 splice isoforms at presynaptic terminals: implications for synaptic vesicle release and synaptic facilitation
- Rodent models for mood disorders – understanding molecular changes by investigating social behavior
- Why do certain cancer cells alter functionality and fuse?
- Research Articles/Short Communications
- Cell Biology and Signaling
- MicroRNA-101-3p inhibits nasopharyngeal carcinoma cell proliferation and cisplatin resistance through ZIC5 down-regulation by targeting SOX2