Startseite Lebenswissenschaften Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer
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Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer

Ein Erratum zu diesem Artikel finden Sie hier: https://doi.org/10.1515/hsz-2015-0237
  • Sangeeta Mehta , Rakhee Chhetra , Radhika Srinivasan , Suresh C. Sharma , Digambar Behera und Sujata Ghosh EMAIL logo
Veröffentlicht/Copyright: 24. März 2013
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 394 Heft 7

Abstract

Maackia amurensis agglutinin is a NeuNAcα (2–3) Galβ (1–4) GlcNAc/Glc-specific lectin, which was shown to have diagnostic potential in cancers of different origin. In a previous report, we demonstrated that GM3 specific IgG from bronchoalveolar lavage fluid (BALF) of non-small cell lung cancer (NSCLC) patients interacted with ∼66kDa membrane glycoprotein band of NSCLC cell lines, which was also recognised by this lectin. This observation prompted us to assess the potential of Maackia amurensis agglutinin in NSCLC. Accordingly, we examined the reactivity of this lectin with NSCLC cell lines as well as the tissue biopsies and cells obtained from fine needle aspirations of NSCLC patients. Maackia amurensis agglutinin showed strong reactivity, specifically with cells and biopsy samples of NSCLC origin. Furthermore, this lectin was found to induce apoptosis in NSCLC cells. The mechanism of this lectin-induced apoptosis involved downregulation of Bcl-XL, upregulation of Bax, release of cytochrome c and activation of procaspase-3. Collectively our results have suggested that Maackia amurensis agglutinin may have the potential to serve as a unique probe for detection of NSCLC and also as a specific apoptosis-inducing agent in NSCLC cells.

Keywords: apoptosis; cytochrome c; glycoprotein; lectin; procaspase-3; sialylation
Corresponding author: Sujata Ghosh, Postgraduate Institute of Medical Education and Research (PGIMER), Department of Experimental Medicine and Biotechnology, Chandigarh 160012, India

This work was supported by a grant from the Indian Council of Medical Research, New Delhi, India.

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Received: 2012-9-2
Accepted: 2013-3-20
Published Online: 2013-3-24
Published in Print: 2013-7-1

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hsz-2012-0279
Schlagwörter für diesen Artikel
apoptosis; cytochrome c; glycoprotein; lectin; procaspase-3; sialylation

Artikel in diesem Heft

  1. Masthead
  2. Masthead
  3. Reviews
  4. Glucocerebrosidase, a new player changing the old rules in Lewy body diseases
  5. Comparison of natural and recombinant tissue factor proteins: new insights
  6. Targeting caspases in cancer therapeutics
  7. When stable RNA becomes unstable: the degradation of ribosomes in bacteria and beyond
  8. Minireviews
  9. Galectins: new agonists of platelet activation
  10. Antitumor effects of energy restriction-mimetic agents: thiazolidinediones
  11. Research Articles/Short Communications
  12. Protein Structure and Function
  13. Biochemical characterization of an S-adenosyl-l-methionine-dependent methyltransferase (Rv0469) of Mycobacterium tuberculosis
  14. Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification
  15. Membranes, Lipids, Glycobiology
  16. Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer
  17. Molecular Medicine
  18. Kinin B1 receptor gene ablation affects hypothalamic CART productionb
  19. Cell Biology and Signaling
  20. Effects of lipotoxicity on a novel insulin-secreting human pancreatic β-cell line, 1.1B4
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