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Kinin B1 receptor gene ablation affects hypothalamic CART productionb

  • Hugo A.M. Torres , Fabiana Louise Motta , Vicencia Micheline Sales , Carolina Batista , Joelcimar M. da Silva , Thiago Vignoli , Gabriela F. Barnabé , Francine O. Goeldner , Vânia D’Almeida , Jackson C. Bittencourt , Rita Sinigaglia-Coimbra , Michael Bader , Luiz Eugênio A.M. Mello and João Bosco Pesquero EMAIL logo
Published/Copyright: March 24, 2013
Biological Chemistry
From the journal Biological Chemistry Volume 394 Issue 7

Abstract

A role for the kinin B1 receptor in energy-homeostatic processes was implicated in previous studies; notably, the studies where kinin B1 receptor knockout mice (B1-/-) were shown to have impaired adiposity, impaired leptin and insulin production, lower feed efficiency, protection from liver steatosis and diet-induced obesity when fed a high fat diet (HFD). In particular, in a model where the B1 receptor is expressed exclusively in the adipose tissue, it rescues the plasma insulin concentration and the weight gain seen in wild type mice. Taking into consideration that leptin participates in the formation of hypothalamic nuclei, which modulate energy expenditure, and feeding behavior, we hypothesized that these brain regions could also be altered in B1-/- mice. We observed for the first time a difference in the gene expression pattern of cocaine and amphetamine related transcript (CART) in the (lateral hypothalamic area (LHA) resulting from the deletion of the kinin B1 receptor gene. The correlation between CART expression in the LHA and the thwarting of diet-induced obesity corroborates independent correlations between CART and obesity. Furthermore, it seems to indicate that the mechanism underlying the ‘lean’ phenotype of B1-/- mice does not stem solely from changes in peripheral tissues but may also receive contributions from changes in the hypothalamic machinery involved in energy homeostasis processes.

Keywords: hypothalamus; kinin B1 receptor; knockout mice; lateral hypothalamic area
Corresponding author: João Bosco Pesquero, Biophysics Department, Universidade Federal de São Paulo (UNIFESP), Rua Pedro de Toledo 669 9oandar, Vila Clementino, 04039-002, Sao Paulo, SP, Brazil

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico Tecnológico (CNPq, 134730/2006-2). HAMT, VD’A, LEM, JCB, and JBP are recipients of fellowships from CNPq, Brazil. GFB and VMS is a recipient of a Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) fellowship. Financial support from FAPESP to JBP (2008)/06676-8) and to JCB (2004)/13849-5) is acknowledged. RSC is a recipient of an Associação Beneficente de Coleta de Sangue/Universidade Federal de São Paulo (COLSAN-UNIFESP) fellowship.

  1. b

    This paper was originally submitted to the Highlight Issue in connection with the ‘Kinin 2012’ symposium (March 2013 issue).

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Received: 2012-10-15
Accepted: 2013-3-20
Published Online: 2013-3-24
Published in Print: 2013-7-1

©2013 by Walter de Gruyter Berlin Boston

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https://doi.org/10.1515/hsz-2012-0302
Keywords for this article
hypothalamus; kinin B1 receptor; knockout mice; lateral hypothalamic area

Articles in the same Issue

  1. Masthead
  2. Masthead
  3. Reviews
  4. Glucocerebrosidase, a new player changing the old rules in Lewy body diseases
  5. Comparison of natural and recombinant tissue factor proteins: new insights
  6. Targeting caspases in cancer therapeutics
  7. When stable RNA becomes unstable: the degradation of ribosomes in bacteria and beyond
  8. Minireviews
  9. Galectins: new agonists of platelet activation
  10. Antitumor effects of energy restriction-mimetic agents: thiazolidinediones
  11. Research Articles/Short Communications
  12. Protein Structure and Function
  13. Biochemical characterization of an S-adenosyl-l-methionine-dependent methyltransferase (Rv0469) of Mycobacterium tuberculosis
  14. Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification
  15. Membranes, Lipids, Glycobiology
  16. Potential importance of Maackia amurensis agglutinin in non-small cell lung cancer
  17. Molecular Medicine
  18. Kinin B1 receptor gene ablation affects hypothalamic CART productionb
  19. Cell Biology and Signaling
  20. Effects of lipotoxicity on a novel insulin-secreting human pancreatic β-cell line, 1.1B4
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