Home Life Sciences Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification
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Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification

  • Antoni Polanowski EMAIL logo , Agnieszka Sosnowska , Agnieszka Zabłocka , Maria Janusz and Tadeusz Trziszka
Published/Copyright: March 14, 2013

Abstract

The protein mixture of cytokine-inducing activity accompanying chicken immunoglobulin Y, named yolkin, consists of several peptides of molecular weight (MW) ranging from over 1 to 35 kDa. Yolkin and its constituent peptides were found to be efficient inducers of interleukin (IL)-1β, IL-6 and IL-10 secretion. N-terminal amino acid sequences of eight of the electrophoretically purified yolkin constituents revealed that all of them are homological to some fragments of the C-terminal domain of vitellogenin II. The fractions of MW about 4 and 12 kDa are free of carbohydrates and start at position 1732 in the vitellogenin amino acid sequence; whereas the other fractions (MW about 16, 19, 23, 29, 32 and 35 kDa) appeared to be glycoproteins corresponding to the amino acid sequence of vitellogenin starting at position 1572. From these data, it is concluded that yolkin most likely represents vitellogenin-derived peptides that possess cytokine-inducing activity and are, at least partially, responsible for such properties of separated immunoglobulin Y preparation. This finding reveals a new role for vitellogenin as a reservoir of polypeptides that may play an important role in the innate immune system of the developing embryo.


Corresponding author: Antoni Polanowski, Faculty of Food Sciences, Wrocław University of Environmental and Life Sciences, ul. Chełmońskiego 37, 51-630 Wrocław, Poland

This study was performed during the implementation of project No. POIG.01.03.01-00-133/08 – ‘Innovative technologies of production of biopreparations based on new generation egg (OVOCURA)’. The project was co-financed by the European Regional Development Fund within the Innovative Economy 2007–2013 Operational Programme.

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Received: 2012-11-26
Accepted: 2013-3-11
Published Online: 2013-3-14
Published in Print: 2013-7-1

©2013 by Walter de Gruyter Berlin Boston

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