The differential role of resistin on invasive liver cancer cells
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Candace Miethe
Abstract
Objectives
To determine whether inhibition of kinase signaling will suppress resistin-induced liver cancer progression. Resistin is located in monocytes and macrophages of adipose tissue. This adipocytokine is an important link between obesity, inflammation, insulin resistance, and cancer risk. Pathways that resistin is known to be involved include but are not limited to mitogen-activated protein kinases (MAPKs) and extracellular signal-regulated kinases (ERK). The ERK pathway promotes cellular proliferation, migration, survival of cancer cells, and tumor progression. The Akt pathway is known to be up-regulated in many cancers including liver cancer.
Methods
Using an in vitro model, HepG2 and SNU-449 liver cancer cells were exposed to resistin ± ERK, Akt, or both inhibitors. The following physiological parameters were assessed: cellular proliferation, ROS, lipogenesis, invasion, MMP, and lactate dehydrogenase activity.
Results
The inhibition of kinase signaling suppressed resistin-induced invasion and lactate dehydrogenase in both cell lines. In addition, in SNU-449 cells, resistin increased proliferation, ROS, and MMP-9 activity. Inhibition of PI3K and ERK decreased phosphorylated Akt and ERK, and pyruvate dehydrogenase.
Conclusions
In this study, we describe the effect of Akt and ERK inhibitors to determine if inhibition suppresses resistin-induced liver cancer progression. Resistin promotes cellular proliferation, ROS, MMP, invasion and LDH activity in SNU-449 liver cancer cells which is differentially mediated by Akt and ERK signaling pathways.
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Research funding: This study was funded internally from Texas State University.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: The conducted research does not include the use of human participants or animals.
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© 2023 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line
- Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy
- Examining the effect of Helicobacter pylori cagPAI variety on gene expression pattern related to gastric cancer
- Plasma IL-6, TREM1, uPAR, and IL6/IL8 biomarkers increment further witnessing the chronic inflammation in type 2 diabetes
- The effect of miR-372-5p regulation on CDX1 and CDX2 in the gastric cancer cell line
- Point-of-care salivary oxidative and renal functional markers to assess kidney function in reperfusion-induced acute kidney injury in male rats
- The differential role of resistin on invasive liver cancer cells
- The association of paraoxonase I gene polymorphisms Q192R (rs662) and L55M (rs854560) and its activity with metabolic syndrome components in fars ethnic group
- Metabolic syndrome – cardiac structure and functional analysis by echocardiography; a cross sectional comparative study
- Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice
- Intermittent vs. continuous swimming training on adipokines and pro-inflammatory cytokines in metabolic syndrome experimental model
- Effect of four-week home-based exercise program on immune response, fat and muscle mass in subjects recovered from COVID-19
- Review Article
- Analytical and therapeutic profiles of DNA methylation alterations in cancer; an overview of changes in chromatin arrangement and alterations in histone surfaces
Artikel in diesem Heft
- Frontmatter
- Original Articles
- The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line
- Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy
- Examining the effect of Helicobacter pylori cagPAI variety on gene expression pattern related to gastric cancer
- Plasma IL-6, TREM1, uPAR, and IL6/IL8 biomarkers increment further witnessing the chronic inflammation in type 2 diabetes
- The effect of miR-372-5p regulation on CDX1 and CDX2 in the gastric cancer cell line
- Point-of-care salivary oxidative and renal functional markers to assess kidney function in reperfusion-induced acute kidney injury in male rats
- The differential role of resistin on invasive liver cancer cells
- The association of paraoxonase I gene polymorphisms Q192R (rs662) and L55M (rs854560) and its activity with metabolic syndrome components in fars ethnic group
- Metabolic syndrome – cardiac structure and functional analysis by echocardiography; a cross sectional comparative study
- Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice
- Intermittent vs. continuous swimming training on adipokines and pro-inflammatory cytokines in metabolic syndrome experimental model
- Effect of four-week home-based exercise program on immune response, fat and muscle mass in subjects recovered from COVID-19
- Review Article
- Analytical and therapeutic profiles of DNA methylation alterations in cancer; an overview of changes in chromatin arrangement and alterations in histone surfaces
