Examining the effect of Helicobacter pylori cagPAI variety on gene expression pattern related to gastric cancer
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Alireza Ahmadzadeh
,
Mohsen Rashidi
Abstract
Objectives
We aimed to determine possible association between heterogeneity of Helicobacter pylori cytotoxin-associated gene pathogenicity island and gene expression profiles in patients with distinct histopathological changes.
Methods
Gastric biopsies were obtained from seventy five patients. Microbiological and pathological examinations were done and intactness of Helicobacter pylori cagPAI was determined by PCR using 11 pairs of primers flanking cagζ-cagA regions and cagPAI empty site. Alterations at mRNA levels of eight genes were investigated by real-time PCR and their association with cagPAI intactness and histopathological changes examined statistically.
Results
A larger proportion of cagPAI positive strains colonized patients with SAG (52.4%), followed by CG (33.3%), and IM (14.3%). Intact cagPAI was found in 87.5% of the strains obtained from patients with SAG, while significantly lower frequency was detected among those with CG (12.5%) and IM (0%). No significant difference was found among the studied histological groups and fold changes in gene expression of gastric biopsies of Helicobacter pylori infected patients with distinct cagPAI status. However, in each histological group, the strains with more complete gene cluster induced (ErbB2, CCNE1, CTNNB1, and MMP7 in SAG and IM groups) or reduced (TP53, in CG group) expression of the GC associated genes in relatively higher levels. APC, TP53 and E-cadherin were down-regulated in patients with SAG and IM compared with CG patients, irrespective to the status of cagPAI integrity.
Conclusions
Helicobacter pylori strains that carry more complete cagPAI segment could induce remarkably higher levels of mRNA changes of GC associated genes in all histopathological groups.
Acknowledgments
This article was supported by Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran. We would like to appreciate all staff of Foodborne and Waterborne Diseases Research Center that helped us for this study.
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Research funding: None declared.
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Author contributions: Alireza Ahmadzadeh do main part of the experiments and wrote initial draft of the manuscript; Mohsen Rashidi cultured the biopsies and the isolates; Zhaleh Mohsenifar performed all pathological examinations; Fariborz Faeghi help in data analysis; Mostafa Rezaei-Tavirani and Nayebali Ahmadi were involved in statistical analysis; Mohammad Reza Zali provided parts of the samples; Masoud Alebouyeh designed the study, supervised the research, reviewed and revised the manuscript; Mehdi feizi and Zobayde Ahmadzadeh were study counselor.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: protocols approved by the ethical review committee at Shahid Beheshti University of Medical Sciences.
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© 2023 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line
- Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy
- Examining the effect of Helicobacter pylori cagPAI variety on gene expression pattern related to gastric cancer
- Plasma IL-6, TREM1, uPAR, and IL6/IL8 biomarkers increment further witnessing the chronic inflammation in type 2 diabetes
- The effect of miR-372-5p regulation on CDX1 and CDX2 in the gastric cancer cell line
- Point-of-care salivary oxidative and renal functional markers to assess kidney function in reperfusion-induced acute kidney injury in male rats
- The differential role of resistin on invasive liver cancer cells
- The association of paraoxonase I gene polymorphisms Q192R (rs662) and L55M (rs854560) and its activity with metabolic syndrome components in fars ethnic group
- Metabolic syndrome – cardiac structure and functional analysis by echocardiography; a cross sectional comparative study
- Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice
- Intermittent vs. continuous swimming training on adipokines and pro-inflammatory cytokines in metabolic syndrome experimental model
- Effect of four-week home-based exercise program on immune response, fat and muscle mass in subjects recovered from COVID-19
- Review Article
- Analytical and therapeutic profiles of DNA methylation alterations in cancer; an overview of changes in chromatin arrangement and alterations in histone surfaces
Artikel in diesem Heft
- Frontmatter
- Original Articles
- The effect of encomir-93 mimic transfection on the expression of miR-93 and PSA and androgen receptor in prostate cancer LNcap cell line
- Association of S19W polymorphism in APOA5 gene and serum lipid levels in patients with type 2 diabetic nephropathy
- Examining the effect of Helicobacter pylori cagPAI variety on gene expression pattern related to gastric cancer
- Plasma IL-6, TREM1, uPAR, and IL6/IL8 biomarkers increment further witnessing the chronic inflammation in type 2 diabetes
- The effect of miR-372-5p regulation on CDX1 and CDX2 in the gastric cancer cell line
- Point-of-care salivary oxidative and renal functional markers to assess kidney function in reperfusion-induced acute kidney injury in male rats
- The differential role of resistin on invasive liver cancer cells
- The association of paraoxonase I gene polymorphisms Q192R (rs662) and L55M (rs854560) and its activity with metabolic syndrome components in fars ethnic group
- Metabolic syndrome – cardiac structure and functional analysis by echocardiography; a cross sectional comparative study
- Investigation of the relationship between betatrophin and certain key enzymes involved in carbohydrate and lipid metabolism in insulin-resistant mice
- Intermittent vs. continuous swimming training on adipokines and pro-inflammatory cytokines in metabolic syndrome experimental model
- Effect of four-week home-based exercise program on immune response, fat and muscle mass in subjects recovered from COVID-19
- Review Article
- Analytical and therapeutic profiles of DNA methylation alterations in cancer; an overview of changes in chromatin arrangement and alterations in histone surfaces
