Startseite Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea
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Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea

  • Nadereh Bakhshandeh , Maryam Mohammadi , Parisa Mohammadi , Elahe Nazari , Mehdi Damchi , Sajad Khodabandelu und Hossein Mokhtari ORCID logo EMAIL logo
Veröffentlicht/Copyright: 30. Dezember 2022
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Hormone Molecular Biology and Clinical Investigation
Aus der Zeitschrift Hormone Molecular Biology and Clinical Investigation Band 44 Heft 2

Abstract

Objectives

Androgen receptor (AR) play a key role in the onset and progression of prostate cancer. Epigallocatechin-3-gallate (EGCG) is a polyphenolic compound and the active ingredient in green tea, which is involved in modulating gene expression through epigenetic alterations. Previous studies have shown that EGCG at low concentrations reduces the expression of AR and prostate-specific antigen (PSA) in the LNCaP cell line of prostate cancer. In this study, the effect of higher EGCG concentrations on AR and PSA expression in LNCaP prostate cancer cell line was investigated.

Methods

In this study, LNCaP prostate cancer cell line was used and after MTT test, concentrations of 40, 60 and 80 μg/mL EGCG were used for treatment. Then, the expression of AR and PSA genes was evaluated by RT-PCR. AR protein expression was also assessed by Western blotting.

Results

The present study showed that treatment of LNCaPs cells by EGCG reduces cell proliferation. The IC50 value was 42.7 μg/mL under experimental conditions. It was also observed that EGCG at concentrations of 40 and 80 μg/mL increased the expression of AR and PSA (p<0.05).

Conclusions

The present study showed that the effect of EGCG on AR expression was different at different concentrations, so that unlike previous studies, higher concentrations of EGCG (80 and 40 μg/mL) increased AR and PSA expression. It seems that due to the toxic effects of EGCG in high concentrations on cancer cells and the possibility of its effect on normal cells, more caution should be exercised in its use.

Keywords: androgen receptor; epigallocatechin epigalate; prostate cancer; prostate specific antigen
Corresponding author: Hossein Mokhtari, Amol Faculty of Paramedicine, Mazandaran University of Medical Sciences, Sari, Iran, Phone: 09113297500, E-mail:

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: Ethical code: TBZmed.REC.1394.618.

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Received: 2022-06-02
Accepted: 2022-12-11
Published Online: 2022-12-30

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Letter to the Editor
  3. The role of gut microbiota in etiopathogenesis of long COVID syndrome
  4. Original Articles
  5. Risk factors and inflammatory markers in acute coronary syndrome-ST elevation myocardial infarction (STEMI)
  6. Association of pro-inflammatory cytokines, inflammatory proteins with atherosclerosis index in obese male subjects
  7. Exploration of meteorin-like peptide (metrnl) predictors in type 2 diabetic patients: the potential role of irisin, and other biochemical parameters
  8. Distinct urinary progesterone metabolite profiles during the luteal phase
  9. Promoter methylation levels of RASSF1 and ATIC genes are associated with lung cancer in Iranian patients
  10. Population status of selenium in Colombia and associated factors: a cross-sectional study
  11. Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes
  12. Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea
  13. The effects of endurance exercise and metformin on memory impairment caused by diabetes
  14. Exercise modulation in inflammation and metabolic hormonal disorders of COVID-19 to decrease risk factors in coronary heart disease
  15. The effect of co-administration of artemisinin and N-acetyl cysteine on antioxidant status, spermatological parameters and histopathology of testis in adult male mice
  16. Case Report
  17. Unilateral ovarian agenesis with ipsilateral tubal presence – report of a case
  18. Review Articles
  19. Brown adipose tissue as an endocrine organ: updates on the emerging role of batokines
  20. Zuranolone and its role in treating major depressive disorder: a narrative review
https://doi.org/10.1515/hmbci-2022-0054
Schlagwörter für diesen Artikel
androgen receptor; epigallocatechin epigalate; prostate cancer; prostate specific antigen

Artikel in diesem Heft

  1. Frontmatter
  2. Letter to the Editor
  3. The role of gut microbiota in etiopathogenesis of long COVID syndrome
  4. Original Articles
  5. Risk factors and inflammatory markers in acute coronary syndrome-ST elevation myocardial infarction (STEMI)
  6. Association of pro-inflammatory cytokines, inflammatory proteins with atherosclerosis index in obese male subjects
  7. Exploration of meteorin-like peptide (metrnl) predictors in type 2 diabetic patients: the potential role of irisin, and other biochemical parameters
  8. Distinct urinary progesterone metabolite profiles during the luteal phase
  9. Promoter methylation levels of RASSF1 and ATIC genes are associated with lung cancer in Iranian patients
  10. Population status of selenium in Colombia and associated factors: a cross-sectional study
  11. Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes
  12. Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea
  13. The effects of endurance exercise and metformin on memory impairment caused by diabetes
  14. Exercise modulation in inflammation and metabolic hormonal disorders of COVID-19 to decrease risk factors in coronary heart disease
  15. The effect of co-administration of artemisinin and N-acetyl cysteine on antioxidant status, spermatological parameters and histopathology of testis in adult male mice
  16. Case Report
  17. Unilateral ovarian agenesis with ipsilateral tubal presence – report of a case
  18. Review Articles
  19. Brown adipose tissue as an endocrine organ: updates on the emerging role of batokines
  20. Zuranolone and its role in treating major depressive disorder: a narrative review
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