Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes

Olusola Olalekan Elekofehinti; Yetunde Victoria Aladenika; Opeyemi Iwaloye; Enoabasi Ima-abasi Okon; Isaac Gbadura Adanlawo

doi:10.1515/hmbci-2022-0053

Article

Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes

Olusola Olalekan Elekofehinti , Yetunde Victoria Aladenika , Opeyemi Iwaloye , Enoabasi Ima-abasi Okon and Isaac Gbadura Adanlawo

Published/Copyright: January 3, 2023

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From the journal Hormone Molecular Biology and Clinical Investigation Volume 44 Issue 2

Abstract

Objectives

There is evidence that mitochondrial dysfunction mediated by hyperglycemia increases the incidence of diabetes and age-related insulin resistance. Thus, maintaining mitochondrial integrity may provide alternative therapeutic approach in diabetes treatment. This study aimed to evaluate the effect of Bambusa vulgaris leaf extract on mitochondrial biogenesis in the pancreas of diabetic rats.

Methods

11 weeks old male rats (n=30) were purchased, and sorted into the following groups: control, diabetic control, diabetes + metformin (100 mg/kg), diabetes + Aq. B. vulgaris (100 mg/kg), diabetes + Aq. B. vulgaris (200 mg/kg), and diabetes + Aq. B. vulgaris (300 mg/kg). Diabetes was induced in the rats by a single dose of 65 mg/kg streptozotocin (STZ). The mRNA expression of genes related to mitochondria biogenesis (pgc-1α, Nrf2, GSK3β, AMPK and SIRT2) and genes of Nrf2-Keap1-ARE signaling pathway were determined by reverse transcriptase polymerase chain reaction. Molecular docking studies including lock and key docking and prime MM-GBSA were incorporated to identify the lead chemical compounds in Bambusa vulgari.

Results

The results showed that B. vulgaris leaf extract promotes mitochondrial biogenesis via altering the mRNA expression of mitochondrial master regulator pgc-1α, other upstream genes, and the Nrf2-Keap1-ARE antioxidant pathway. Through molecular docking results, cryptochlorogenic acid, hesperidin, orientin, vitexin, scopolin, and neochlorogenic were found as the crucial chemicals in B. vulgaris with the most modulating effect on PGC-1α, AMPK, and GSK3.

Conclusions

This study thus suggests that B. vulgaris leaf extract restores the integrity of mitochondria in diabetic rats.

Keywords: B. vulgaris leaf extract; diabetes mellitus; mitochondria biogenesis; Nrf2-Keap1-ARE; pgc-1α

Corresponding author: Olusola Olalekan Elekofehinti, Bioinformatics and Molecular Biology Unit, Department of Biochemistry, Federal University of Technology Akure, Akure, Ondo State, Nigeria, E-mail: ooelekofehinti@futa.edu.ng

Conflict of interest: The authors declare no conflict of interest.
Ethics approval statements: The experiments were performed in accordance with the National Guidelines for Experimental Animal Welfare and with approval of the Animal Welfare and Research Ethics Committee at Federal university of Technology, Akure, Nigeria.

References

1. Shaw, JE, Sicree, RA, Zimmet, PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4–14. https://doi.org/10.1016/j.diabres.2009.10.007.Search in Google Scholar PubMed

2. Makinde, EA, Radenahmad, N, Adekoya, AE, Olatunji, OJ. Tiliacora triandra extract possesses antidiabetic effects in high fat diet/streptozotocin-induced diabetes in rats. J Food Biochem 2020;44:e13239. https://doi.org/10.1111/jfbc.13239.Search in Google Scholar PubMed

3. Ozougwu, JC, Obimba, KC, Belonwu, CD, Unakalamba, CB. The pathogenesis and pathophysiology of type 1 and type 2 diabetes mellitus. J Physiol Pathophysiol 2013;4:46–57. https://doi.org/10.5897/jpap2013.0001.Search in Google Scholar

4. Cade, WT. Diabetes-related microvascular and macrovascular diseases in the physical therapy setting. Phys Ther 2008;88:1322–35. https://doi.org/10.2522/ptj.20080008.Search in Google Scholar PubMed PubMed Central

5. Chawla, A, Chawla, R, Jaggi, S. Microvasular and macrovascular complications in diabetes mellitus: distinct or continuum? Indian J Endocrinol Metabol 2016;20:546. https://doi.org/10.4103/2230-8210.183480.Search in Google Scholar PubMed PubMed Central

6. van der Bliek, AM, Shen, Q, Kawajiri, S. Mechanisms of mitochondrial fission and fusion. Cold Spring Harbor Perspect Biol 2013;5:a011072. https://doi.org/10.1101/cshperspect.a011072.Search in Google Scholar PubMed PubMed Central

7. Elekofehinti, OO, Ayodele, OC, Iwaloye, O. Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study. Egypt J Med Hum Genets 2021;22:1–11. https://doi.org/10.1186/s43042-021-00200-w.Search in Google Scholar

8. Twig, G, Elorza, A, Molina, AJA, Mohamed, H, Wikstrom, JD, Walzer, G, et al.. Fission and selective fusion govern mitochondrial segregation and elimination by autophagy. EMBO J 2008;27:433–46. https://doi.org/10.1038/sj.emboj.7601963.Search in Google Scholar PubMed PubMed Central

9. Guo, C, Sun, L, Chen, X, Zhang, D. Oxidative stress, mitochondrial damage and neurodegenerative diseases. Neural Regen Res 2013;8:2003.Search in Google Scholar

10. Picca, A, Guerra, F, Calvani, R, Coelho-Junior, HJ, Bossola, M, Landi, F, et al.. Generation and release of mitochondrial-derived vesicles in health, aging and disease. J Clin Med 2020;9:1440. https://doi.org/10.3390/jcm9051440.Search in Google Scholar PubMed PubMed Central

11. Cheng, CF, Ku, HC, Lin, H. PGC-1α as a pivotal factor in lipid and metabolic regulation. Int J Mol Sci 2018;19:3447. https://doi.org/10.3390/ijms19113447.Search in Google Scholar PubMed PubMed Central

12. Luo, X, Liao, C, Quan, J, Cheng, C, Zhao, X, Bode, AM, et al.. Posttranslational regulation of PGC‐1α and its implication in cancer metabolism. Int J Cancer 2019;145:1475–83. https://doi.org/10.1002/ijc.32253.Search in Google Scholar PubMed PubMed Central

13. Gureev, AP, Shaforostova, EA, Popov, VN. Regulation of mitochondrial biogenesis as a way for active longevity: interaction between the Nrf2 and PGC-1α signaling pathways. Front Genet 2019;10:435. https://doi.org/10.3389/fgene.2019.00435.Search in Google Scholar PubMed PubMed Central

14. Yeasmin, L, Ali, M, Gantait, S, Chakraborty, S. Bamboo: an overview on its genetic diversity and characterization. 3 Biotechnol 2015;5:1–11. https://doi.org/10.1007/s13205-014-0201-5.Search in Google Scholar PubMed PubMed Central

15. Yakubu, MT, Bukoye, BB. Abortifacient potentials of the aqueous extract of Bambusa vulgaris leaves in pregnant Dutch rabbits. Contraception 2009;80:308–13. https://doi.org/10.1016/j.contraception.2009.03.003.Search in Google Scholar PubMed

16. Meliani, N, Dib, MEA, Allali, H, Tabti, B. Hypoglycaemic effect of Berberis vulgaris L. in normal and streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 2011;1:468–71. https://doi.org/10.1016/s2221-1691(11)60102-0.Search in Google Scholar PubMed PubMed Central

17. Patti, ME, Butte, AJ, Crunkhorn, S, Cusi, K, Berria, R, Kashyap, S, et al.. Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1. Proc Natl Acad Sci USA 2003;100:8466–71. https://doi.org/10.1073/pnas.1032913100.Search in Google Scholar PubMed PubMed Central

18. Mootha, VK, Lindgren, CM, Eriksson, KF, Subramanian, A, Sihag, S, Lehar, J, et al.. PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet 2003;34:267–73. https://doi.org/10.1038/ng1180.Search in Google Scholar PubMed

19. Zhang, Z, Zhang, X, Meng, L, Gong, M, Li, J, Shi, W, et al.. Pioglitazone inhibits diabetes-induced atrial mitochondrial oxidative stress and improves mitochondrial biogenesis, dynamics, and function through the PPAR-γ/PGC-1α signaling pathway. Front Pharmacol 2021;12:658362. https://doi.org/10.3389/fphar.2021.658362.Search in Google Scholar PubMed PubMed Central

20. Panee, J. Potential medicinal application and toxicity evaluation of extracts from bamboo plants. J Med Plants Res 2015;9:681. https://doi.org/10.5897/jmpr2014.5657.Search in Google Scholar PubMed PubMed Central

21. Wróblewska, KB, de Oliveira, DCS, Grombone-Guaratini, MT, Moreno, PRH. Medicinal Properties of Bamboos. In: Pharmacognosy: Medicinal Plants. IntechOpen, London, UK. ISBN: 978-1-83880-611-8 2019;1–18.Search in Google Scholar

22. jin, FW, Wangjiang, C, He, Y, Zhoulu, Y, Pengdong, X, Liu, S. Resveratrol alleviates diabetic cardiomyopathy in rats by improving mitochondrial function through PGC-1α deacetylation. Acta Pharmacol Sin 2018;39:59–73. https://doi.org/10.1038/aps.2017.50.Search in Google Scholar PubMed PubMed Central

23. Cantó, C, Auwerx, J. PGC-1alpha, SIRT1 and AMPK, an energy sensing network that controls energy expenditure. Curr Opin Lipidol 2009;20:98. https://doi.org/10.1097/mol.0b013e328328d0a4.Search in Google Scholar

24. Fernandez-Marcos, PJ, Auwerx, J. Regulation of PGC-1α, a nodal regulator of mitochondrial biogenesis. Am J Clin Nutr 2011;93:884S–90S. https://doi.org/10.3945/ajcn.110.001917.Search in Google Scholar PubMed PubMed Central

25. Bao, J, Sack, MN. Protein deacetylation by sirtuins: delineating a post-translational regulatory program responsive to nutrient and redox stressors. Cell Mol Life Sci 2010;67:3073–87. https://doi.org/10.1007/s00018-010-0402-y.Search in Google Scholar PubMed PubMed Central

26. Iside, C, Scafuro, M, Nebbioso, A, Altucci, L. SIRT1 activation by natural phytochemicals: an overview. Front Pharmacol 2020;11:1225. https://doi.org/10.3389/fphar.2020.01225.Search in Google Scholar PubMed PubMed Central

27. Zhang, F, Feng, J, Zhang, J, Kang, X, Qian, D. Quercetin modulates AMPK/SIRT1/NF-κB signaling to inhibit inflammatory/oxidative stress responses in diabetic high fat diet-induced atherosclerosis in the rat carotid artery. Exp Ther Med 2020;20:1. https://doi.org/10.3892/etm.2020.9410.Search in Google Scholar PubMed PubMed Central

28. Yang, K, Chen, Z, Gao, J, Shi, W, Li, L, Jiang, S, et al.. The key roles of GSK-3β in regulating mitochondrial activity. Cell Physiol Biochem 2017;44:1445–59. https://doi.org/10.1159/000485580.Search in Google Scholar PubMed

29. Sun, Q, Jia, N, Li, X, Yang, J, Chen, G. Grape seed proanthocyanidins ameliorate neuronal oxidative damage by inhibiting GSK-3β-dependent mitochondrial permeability transition pore opening in an experimental model of sporadic Alzheimer’s disease. Aging 2019;11:4107. https://doi.org/10.18632/aging.102041.Search in Google Scholar PubMed PubMed Central

30. Undi, RB, Gutti, U, Gutti, RK. LiCl regulates mitochondrial biogenesis during megakaryocyte development. J Trace Elem Med Biol 2017;39:193–201. https://doi.org/10.1016/j.jtemb.2016.10.003.Search in Google Scholar PubMed

31. Wang, D, Liu, L, Zhu, X, Wu, W, Wang, Y. Hesperidin alleviates cognitive impairment, mitochondrial dysfunction and oxidative stress in a mouse model of Alzheimer’s disease. Cell Mol Neurobiol 2014;34:1209–21. https://doi.org/10.1007/s10571-014-0098-x.Search in Google Scholar PubMed

32. Dai, P, Mao, Y, Sun, X, Li, X, Muhammad, I, Gu, W, et al.. Attenuation of oxidative stress-induced osteoblast apoptosis by curcumin is associated with preservation of mitochondrial functions and increased Akt-GSK3β signaling. Cell Physiol Biochem 2017;41:661–77. https://doi.org/10.1159/000457945.Search in Google Scholar PubMed

33. Zhang, Y, Jiao, J, Liu, C, Wu, X, Zhang, Y. Isolation and purification of four flavone C-glycosides from antioxidant of bamboo leaves by macroporous resin column chromatography and preparative high-performance liquid chromatography. Food Chem 2008;107:1326–36.10.1016/j.foodchem.2007.09.037Search in Google Scholar

34. Lee, HC, Wei, YH. Mitochondrial biogenesis and mitochondrial DNA maintenance of mammalian cells under oxidative stress. Int J Biochem Cell Biol 2005;37:822–34. https://doi.org/10.1016/j.biocel.2004.09.010.Search in Google Scholar PubMed

35. Kim, J, Wei, Y, Sowers, JR. Role of mitochondrial dysfunction in insulin resistance. Circ Res 2008;102:401–14. https://doi.org/10.1161/circresaha.107.165472.Search in Google Scholar PubMed PubMed Central

36. Uruno, A, Yagishita, Y, Yamamoto, M. The Keap1–Nrf2 system and diabetes mellitus. Arch Biochem Biophys 2015;566:76–84. https://doi.org/10.1016/j.abb.2014.12.012.Search in Google Scholar PubMed

37. David, JA, Rifkin, WJ, Rabbani, PS, Ceradini, DJ. The Nrf2/Keap1/ARE pathway and oxidative stress as a therapeutic target in type II diabetes mellitus. J Diabetes Res 2017;2017:1–15. https://doi.org/10.1155/2017/4826724.Search in Google Scholar PubMed PubMed Central

38. Iwaloye, O, Elekofehinti, OO, Kikiowo, B, Oluwarotimi, EA, Fadipe, TM. Machine learning-based virtual screening strategy RevealsSome natural compounds as potential PAK4 inhibitors in triple negative breast cancer. Curr Proteonomics 2021;18:753–69. https://doi.org/10.2174/1570164618999201223092209.Search in Google Scholar

Received: 2022-05-28

Accepted: 2022-12-11

Published Online: 2023-01-03

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Articles in the same Issue

https://doi.org/10.1515/hmbci-2022-0053

Keywords for this article

B. vulgaris leaf extract; diabetes mellitus; mitochondria biogenesis; Nrf2-Keap1-ARE; pgc-1α