Promoter methylation levels of RASSF1 and ATIC genes are associated with lung cancer in Iranian patients
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Mahsa Mashayekhi
Abstract
Objectives
Epigenetic alterations like methylation of tumor suppressor genes or oncogenes, in respiratory epithelium have been associated with lung cancer. Hypermethylation of genes promoter is an epigenetic event, and is responsible to tumor suppressor genes inactivation as well as oncogenes activation. This study aimed to assess the role of methylation status in promoter of RASSF1 and ATIC genes their potential implication in the pathogenesis of lung tumor in Iranian patients.
Methods
In this study, we collected 100 tissue samples (50 lung cancer tissues and 50 adjacent non-cancerous lung tissues) from Iranian lung cancer patients. The genomic DNA was extracted, and methylation status of both RASSF1 and ATIC genes was investigated by methylation-sensitive high-resolution melting (MS-HRM) assay technique and Real-Time PCR. Cancer Genome Atlas (TCGA) dataset was also analyzed for further validation of the gene’s methylation.
Results
Methylation of RASSF1 gene promoter was significantly higher in lung tumor tissues. However, promoter methylation levels of ATIC gene was significantly lower in lung tumor tissues. These results were additionally confirmed by TCGA analysis. Promoter methylation of both RASSF1 and ATIC genes was significantly associated with lymph node metastasis, and clinical stage of lung cancer. The receiver operating characteristic (ROC) curve analysis indicated a high accuracy of promoter methylation in these genes as a diagnostic biomarker for lung cancer.
Conclusions
Methylation levels of both RASSF1 and ATIC genes promoters were associated with lung cancer pathogenesis in Iranian population, and may be a suitable biomarker for diagnosis and prognosis of lung cancer in early stage of tumorigenesis.
Funding source: Tabriz University of Medical Sciences
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Research funding: This work was supported by Tabriz University of Medical Sciences.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: The local Institutional Review Board deemed the study exempt from review.
References
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- The role of gut microbiota in etiopathogenesis of long COVID syndrome
- Original Articles
- Risk factors and inflammatory markers in acute coronary syndrome-ST elevation myocardial infarction (STEMI)
- Association of pro-inflammatory cytokines, inflammatory proteins with atherosclerosis index in obese male subjects
- Exploration of meteorin-like peptide (metrnl) predictors in type 2 diabetic patients: the potential role of irisin, and other biochemical parameters
- Distinct urinary progesterone metabolite profiles during the luteal phase
- Promoter methylation levels of RASSF1 and ATIC genes are associated with lung cancer in Iranian patients
- Population status of selenium in Colombia and associated factors: a cross-sectional study
- Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes
- Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea
- The effects of endurance exercise and metformin on memory impairment caused by diabetes
- Exercise modulation in inflammation and metabolic hormonal disorders of COVID-19 to decrease risk factors in coronary heart disease
- The effect of co-administration of artemisinin and N-acetyl cysteine on antioxidant status, spermatological parameters and histopathology of testis in adult male mice
- Case Report
- Unilateral ovarian agenesis with ipsilateral tubal presence – report of a case
- Review Articles
- Brown adipose tissue as an endocrine organ: updates on the emerging role of batokines
- Zuranolone and its role in treating major depressive disorder: a narrative review
Artikel in diesem Heft
- Frontmatter
- Letter to the Editor
- The role of gut microbiota in etiopathogenesis of long COVID syndrome
- Original Articles
- Risk factors and inflammatory markers in acute coronary syndrome-ST elevation myocardial infarction (STEMI)
- Association of pro-inflammatory cytokines, inflammatory proteins with atherosclerosis index in obese male subjects
- Exploration of meteorin-like peptide (metrnl) predictors in type 2 diabetic patients: the potential role of irisin, and other biochemical parameters
- Distinct urinary progesterone metabolite profiles during the luteal phase
- Promoter methylation levels of RASSF1 and ATIC genes are associated with lung cancer in Iranian patients
- Population status of selenium in Colombia and associated factors: a cross-sectional study
- Bambusa vulgaris leaves reverse mitochondria dysfunction in diabetic rats through modulation of mitochondria biogenic genes
- Increased expression of androgen receptor and PSA genes in LNCaP (prostate cancer) cell line due to high concentrations of EGCG, an active ingredient in green tea
- The effects of endurance exercise and metformin on memory impairment caused by diabetes
- Exercise modulation in inflammation and metabolic hormonal disorders of COVID-19 to decrease risk factors in coronary heart disease
- The effect of co-administration of artemisinin and N-acetyl cysteine on antioxidant status, spermatological parameters and histopathology of testis in adult male mice
- Case Report
- Unilateral ovarian agenesis with ipsilateral tubal presence – report of a case
- Review Articles
- Brown adipose tissue as an endocrine organ: updates on the emerging role of batokines
- Zuranolone and its role in treating major depressive disorder: a narrative review
