Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells
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Nazlı Çil
,
Mutlu Yaka
Abstract
Objectives
Stem cell treatment is based on Melatonin which is crucial for lots of pathological and physiological pathways. Our aim is determining the most appropriate dose of melatonin affecting the rat adipose tissue mesenchymal stem cells.
Methods
Stem cells were isolated from male rat adipose tissue. Differentiation and characterization experiments were performed. Cell viability analyses in stem cells were used the XTT [2,3-Bis-(2-methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide] assay. After 24 h incubation, different concentrations (0.5, 1, 5, 10, 50 µM) of extract were treated to the stem cells for 24 h, 48 and 72 h considering time and dose dependent manner. Total antioxidant status (TAS) and the total oxidant status (TOS) in control cells and melatonin treated cells (5, 10 µM) were determined Rel Assay commercial kits.
Results
In 24 h, melatonin increased cell viability in all groups. When we evaluate the effect of melatonin in 48 h, the most proliferation increase was seen at 5, 10 µM doses. When the total oxidant activity melatonin was found to be significantly lower in 5 and 10 µM dose groups of melatonin.
Conclusions
Melatonin increases the survivor of stem cells and the most effective dose is 5 and 10 µM. The reduction of the oxidative stress index as a result of treating melatonin to mesenchymal stem cells showed that melatonin is a powerful antioxidant for stem cells.
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Research funding: The study was carried out using consumables in our laboratory.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed content: Not applicable.
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Ethical approval: The ethical approval from the Animal Experiments Ethics Committee of Pamukkale University was used in this study with the number PAU HADYEK-2019/20.
References
1. Dominici, M, Le Blanc, K, Mueller, I, Slaper-Cortenbach, I, Marini, F, Krause, D, et al.. Minimal criteria for defining multipotent mesenchymal stromal cells. The international society for cellular therapy position statement. Cytotherapy 2006;8:315–7. https://doi.org/10.1080/14653240600855905. PMID: 16923606.Suche in Google Scholar PubMed
2. Si, Z, Wang, X, Sun, C, Kang, Y, Xu, J, Wang, X, et al.. Adipose-derived stem cells: sources, potency, and implications for regenerative therapies. Biomed Pharmacother 2019;114:108765. https://doi.org/10.1016/j.biopha.2019.108765. Epub 2019 Mar 25. PMID: 30921703.Suche in Google Scholar PubMed
3. Heo, JS, Pyo, S, Lim, JY, Yoon, DW, Kim, BY, Kim, JH, et al.. Biological effects of melatonin on human adipose-derived mesenchymal stem cells. Int J Mol Med 2019;44:2234–44. https://doi.org/10.3892/ijmm.2019.4356. Epub 2019 Sep 27. PMID: 31573052; PMCID: PMC6844604.Suche in Google Scholar PubMed PubMed Central
4. Shuai, Y, Liao, L, Su, X, Yu, Y, Shao, B, Jing, H, et al.. Melatonin treatment improves mesenchymal stem cells therapy by preserving stemness during long-term in vitro expansion. Theranostics 2016;6:1899–917. https://doi.org/10.7150/thno.15412. PMID: 27570559; PMCID: PMC4997245.Suche in Google Scholar PubMed PubMed Central
5. Li, H, Zhang, Y, Liu, S, Li, F, Wang, B, Wang, J, et al.. Melatonin enhances proliferation and modulates differentiation of neural stem cells via autophagy in hyperglycemia. Stem Cell 2019;37:504–15. https://doi.org/10.1002/stem.2968. Epub 2019 Jan 14. PMID: 30644149.Suche in Google Scholar PubMed
6. Rafat, A, Roushandeh, AM, Alizadeh, A, Hashemi-Firouzi, N, Golipoor, Z. Comparison of the melatonin preconditioning efficacy between bone marrow and adipose-derived mesenchymal stem cells. Cell J 2019;20:450–8. https://doi.org/10.22074/cellj.2019.5507. Epub 2018 Aug 1. PMID: 30123990; PMCID: PMC6099139.Suche in Google Scholar PubMed PubMed Central
7. Tan, SS, Han, X, Sivakumaran, P, Lim, SY, Morrison, WA. Melatonin protects human adipose-derived stem cells from oxidative stress and cell death. Arch Plast Surg 2016;43:237–41. https://doi.org/10.5999/aps.2016.43.3.237. Epub 2016 May 18. PMID: 27218020; PMCID: PMC4876151.Suche in Google Scholar PubMed PubMed Central
8. Lee, JH, Han, YS, Lee, SH. Potentiation of biological effects of mesenchymal stem cells in ischemic conditions by melatonin via upregulation of cellular prion protein expression. J Pineal Res 2017;62:e12385. https://doi.org/10.1111/jpi.12385. Epub 2017 Jan 30. Erratum in: J Pineal Res. 2020 May;68(4):e12649. PMID: 28095625.Suche in Google Scholar PubMed
9. Fu, J, Zhao, SD, Liu, HJ, Yuan, QH, Liu, SM, Zhang, YM, et al.. Melatonin promotes proliferation and differentiation of neural stem cells subjected to hypoxia in vitro. J Pineal Res 2011;51:104–12. https://doi.org/10.1111/j.1600-079X.2011.00867.x. Epub 2011 Mar 11. PMID: 21392094.Suche in Google Scholar PubMed
10. Zhang, L, Su, P, Xu, C, Chen, C, Liang, A, Du, K, et al.. Melatonin inhibits adipogenesis and enhances osteogenesis of human mesenchymal stem cells by suppressing PPARγ expression and enhancing Runx2 expression. J Pineal Res 2010;49:364–72. https://doi.org/10.1111/j.1600-079X.2010.00803.x. Epub 2010 Aug 24. PMID: 20738756.Suche in Google Scholar PubMed
11. Rashed, LA, Elattar, S, Eltablawy, N, Ashour, H, Mahmoud, LM, El-Esawy, Y. Mesenchymal stem cells pretreated with melatonin ameliorate kidney functions in a rat model of diabetic nephropathy. Biochem Cell Biol 2018;96:564–71. https://doi.org/10.1139/bcb-2017-0230. Epub 2018 Feb 9. PMID: 29425466.Suche in Google Scholar PubMed
12. Mias, C, Trouche, E, Seguelas, MH, Calcagno, F, Dignat-George, F, Sabatier, F, et al.. Ex vivo pretreatment with melatonin improves survival, proangiogenic/mitogenic activity, and efficiency of mesenchymal stem cells injected into ischemic kidney. Stem Cell 2008;26:1749–57. https://doi.org/10.1634/stemcells.2007-1000. Epub 2008 May 8. PMID: 18467662.Suche in Google Scholar PubMed
13. Zhou, L, Chen, X, Liu, T, Gong, Y, Chen, S, Pan, G, et al.. Melatonin reverses H2O2-induced premature senescence in mesenchymal stem cells via the SIRT1-dependent pathway. J Pineal Res 2015;59:190–205. https://doi.org/10.1111/jpi.12250. Epub 2015 Jul 7. PMID: 25975679; PMCID: PMC4523475.Suche in Google Scholar PubMed PubMed Central
14. Winiarska, K, Dzik, JM, Labudda, M, Focht, D, Sierakowski, B, Owczarek, A, et al.. Melatonin nephroprotective action in Zucker diabetic fatty rats involves its inhibitory effect on NADPH oxidase. J Pineal Res 2016;60:109–17. https://doi.org/10.1111/jpi.12296. Epub 2015 Nov 20. PMID: 26514550.Suche in Google Scholar PubMed
15. Zhao, L, Hu, C, Zhang, P, Jiang, H, Chen, J. Melatonin preconditioning is an effective strategy for mesenchymal stem cell-based therapy for kidney disease. J Cell Mol Med 2020;24:25–33. https://doi.org/10.1111/jcmm.14769. Epub 2019 Nov 20. PMID: 31747719; PMCID: PMC6933322.Suche in Google Scholar PubMed PubMed Central
16. Luchetti, F, Canonico, B, Bartolini, D, Arcangeletti, M, Ciffolilli, S, Murdolo, G, et al.. Melatonin regulates mesenchymal stem cell differentiation: a review. J Pineal Res 2014;56:382–97. https://doi.org/10.1111/jpi.12133. Epub 2014 Apr 15. PMID: 24650016.Suche in Google Scholar PubMed
17. Wang, FW, Wang, Z, Zhang, YM, Du, ZX, Zhang, XL, Liu, Q, et al.. Protective effect of melatonin on bone marrow mesenchymal stem cells against hydrogen peroxide-induced apoptosis in vitro. J Cell Biochem 2013;114:2346–55. https://doi.org/10.1002/jcb.24582. PMID: 23824714.Suche in Google Scholar PubMed
18. Zhu, P, Liu, J, Shi, J, Zhou, Q, Liu, J, Zhang, X, et al.. Melatonin protects ADSCs from ROS and enhances their therapeutic potency in a rat model of myocardial infarction. J Cell Mol Med 2015;19:2232–43. https://doi.org/10.1111/jcmm.12610. Epub 2015 Jun 17. PMID: 26081690; PMCID: PMC4568927.Suche in Google Scholar PubMed PubMed Central
19. Lee, SJ, Jung, YH, Oh, SY, Yun, SP, Han, HJ. Melatonin enhances the human mesenchymal stem cells motility via melatonin receptor 2 coupling with Gαq in skin wound healing. J Pineal Res 2014;57:393–407. https://doi.org/10.1111/jpi.12179. Epub 2014 Oct 7. PMID: 25250716.Suche in Google Scholar PubMed
20. Chang, CL, Sung, PH, Sun, CK, Chen, CH, Chiang, HJ, Huang, TH, et al.. Protective effect of melatonin-supported adipose-derived mesenchymal stem cells against small bowel ischemia-reperfusion injury in rat. J Pineal Res. 2015;59:206–20. https://doi.org/10.1111/jpi.12251. Epub 2015 Jun 24. PMID: 26013733.Suche in Google Scholar PubMed
21. Chen, HH, Lin, KC, Wallace, CG, Chen, YT, Yang, CC, Leu, S, et al.. Additional benefit of combined therapy with melatonin and apoptotic adipose-derived mesenchymal stem cell against sepsis-induced kidney injury. J Pineal Res. 2014;57:16–32. https://doi.org/10.1111/jpi.12140. Epub 2014 May 15. PMID: 24761983.Suche in Google Scholar PubMed
22. Yun, SP, Han, YS, Lee, JH, Kim, SM, Lee, SH. Melatonin rescues mesenchymal stem cells from senescence induced by the uremic toxin p-cresol via inhibiting mTOR-dependent autophagy. Biomol Ther (Seoul) 2018;26:389–98. https://doi.org/10.4062/biomolther.2017.071. PMID: 28655071; PMCID: PMC6029684.Suche in Google Scholar PubMed PubMed Central
23. Gutierrez-Cuesta, J, Tajes, M, Jiménez, A, Coto-Montes, A, Camins, A, Pallàs, M. Evaluation of potential pro-survival pathways regulated by melatonin in a murine senescence model. J Pineal Res 2008;45:497–505. https://doi.org/10.1111/j.1600-079X.2008.00626.x. Epub 2008 Aug 13. PMID: 18705649.Suche in Google Scholar PubMed
24. Liu, X, Xu, Y, Chen, S, Tan, Z, Xiong, K, Li, Y, et al.. Rescue of proinflammatory cytokine-inhibited chondrogenesis by the antiarthritic effect of melatonin in synovium mesenchymal stem cells via suppression of reactive oxygen species and matrix metalloproteinases. Free Radic Biol Med 2014;68:234–46. https://doi.org/10.1016/j.freeradbiomed.2013.12.012. Epub 2013 Dec 25. PMID: 24374373.Suche in Google Scholar PubMed
25. Hu, C, Li, L. Melatonin plays critical role in mesenchymal stem cell-based regenerative medicine in vitro and in vivo. Stem Cell Res Ther 2019;10:13. https://doi.org/10.1186/s13287-018-1114-8. PMID: 30635065; PMCID: PMC6329089.Suche in Google Scholar PubMed PubMed Central
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Letters to the Editor
- The solution to obesity and type 2 diabetes: reversing insulin resistance
- The second wave of COVID-19: effect on Indian health care system
- Original Articles
- Association between vitamin D level and prognostic factors among patients infected with SARS-CoV-2
- The association between serum vitamin D and mood disorders in a cohort of lipedema patients
- Inhibition of PI3K/Akt and ERK signaling decreases visfatin-induced invasion in liver cancer cells
- The outcome of intra-cytoplasmic sperm injection (ICSI): do the sperm concentration and motility matter?
- Effect of two hypercaloric diets on the hormonal and metabolic profile of the adrenal gland
- Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players
- A fine tuning of embryo loading technique by eliminating the air bubble in the outer sheath of embryo catheter among women undergoing IVF treatment
- Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells
- PTEN protein expression has role in predicting disease-free-interval in endometrioid endometrial carcinoma
- Amelioration of STZ-induced nephropathy in diabetic rats by saffron hydro alcoholic extract
- Elevated levels of neopterin and pentraxin 3 in patients with rheumatoid arthritis
- Case Reports
- COVID-19 transmission: a positive perspective
- Distant recurrence of endometrial cancer more than 10 years after hysterectomy: a case report
- Review Articles
- COVID-19 and lung pathologies
- Potential role of melatonin in prevention and treatment of leukaemia
Artikel in diesem Heft
- Frontmatter
- Letters to the Editor
- The solution to obesity and type 2 diabetes: reversing insulin resistance
- The second wave of COVID-19: effect on Indian health care system
- Original Articles
- Association between vitamin D level and prognostic factors among patients infected with SARS-CoV-2
- The association between serum vitamin D and mood disorders in a cohort of lipedema patients
- Inhibition of PI3K/Akt and ERK signaling decreases visfatin-induced invasion in liver cancer cells
- The outcome of intra-cytoplasmic sperm injection (ICSI): do the sperm concentration and motility matter?
- Effect of two hypercaloric diets on the hormonal and metabolic profile of the adrenal gland
- Investigation of the effect of training on serotonin, melatonin and hematologic parameters in adolescent basketball players
- A fine tuning of embryo loading technique by eliminating the air bubble in the outer sheath of embryo catheter among women undergoing IVF treatment
- Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells
- PTEN protein expression has role in predicting disease-free-interval in endometrioid endometrial carcinoma
- Amelioration of STZ-induced nephropathy in diabetic rats by saffron hydro alcoholic extract
- Elevated levels of neopterin and pentraxin 3 in patients with rheumatoid arthritis
- Case Reports
- COVID-19 transmission: a positive perspective
- Distant recurrence of endometrial cancer more than 10 years after hysterectomy: a case report
- Review Articles
- COVID-19 and lung pathologies
- Potential role of melatonin in prevention and treatment of leukaemia
