The effects of follicle-stimulating hormone receptor (FSHR) -29 and Ser680Asn polymorphisms in IVF/ICSI
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Chrysa Paschalidou
Abstract
Background
The aim of this study was to analyze two different polymorphisms, Ser680Asn and -29 (G>A) promoter polymorphism, of the follicle-stimulating hormone receptor (FSHR) gene, individually but also in combination, in a sample of Greek women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI).
Materials and methods
One hundred and forty-one women undergoing IVF or ICSI and 94 controls were genotyped by real-time polymerase chain reaction (RT-PCR) for the two FSHR polymorphisms. The association of the alleles with the clinical, biochemical and other parameters concerning the controlled ovarian stimulation (COS) protocol and outcome was investigated, as well as the pregnancy rate.
Results
The study of each polymorphism individually revealed a positive correlation of the SerSer genotype (Ser680Asn polymorphism) with higher luteinizing hormone (LH) levels on the third day of the menstrual cycle. On the other hand, the A allele for the -29 (G>A) promoter polymorphism correlated with the increased number and quality of cumulus-oocyte complexes (COCs). No differences were detected when the different genotypes of the two polymorphisms were combined – the population study was grouped according to the number of polymorphic alleles they carried (0–4 alleles). Women who presented all polymorphic alleles, AsnAsn/AA, exhibited the lowest LH levels (2.62 ± 0.68 mIU/L), but were rarely detected (n = 2, 1.4% of the studied population).
Conclusions
The data from this study reflect that the investigation of the combination of polymorphisms, such as FSHR -29 and Ser680Asn, could offer a valuable tool in order to evaluate and anticipate the outcome of the ovulation induction protocols, especially in the group of patients with failed attempts.
Author statement
Research funding: Authors state no funding involved.
Conflict of interest: Authors state no conflict of interest.
Informed consent: All patients signed a consent form, approved by the hospitals’ Ethical Committee.
Ethical approval: The study received approval from the hospital’s board.
Author contributions: (I) Conception and design: E. Anagnostou, D. Loutradis; (II) Collection and assembly of data: C. Paschalidou, E. Anagnostou, D. Mavrogianni, N. Klimis; (III) Data analysis and interpretation: D. Mavrogianni, R. Raouasnte, P. Drakakis; (IV) Manuscript writing: all authors; (V) Final approval of the manuscript: all authors.
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Artikel in diesem Heft
- Letters to the Editor
- Androgen-driven COVID-19 infection – is testosterone an enemy or a friend?
- Tumor necrosis factor-α gene-1031T/C promoter polymorphism and endometriosis
- Original Articles
- Serum FGF-21 and FGF-23 in association with gestational diabetes: a longitudinal case-control study
- Neutrophil and monocyte ratios to high-density lipoprotein-cholesterol and adiponectin as biomarkers of nascent metabolic syndrome
- Predicting long-term cardiovascular outcomes of patients with acute myocardial infarction using soluble ST2
- Anti-inflammatory activity of emu oil-based nanofibrous scaffold through downregulation of IL-1, IL-6, and TNF-α pro-inflammatory cytokines
- A comparative study of the antidiabetic effect of two training protocols in streptozotocin-nicotinamide diabetic rats
- Gonadotrophins modulate cell death-related genes expression in human endometrium
- Exploring the activity of the enzyme 11β-hydroxylase in the polycystic ovary syndrome
- The effects of follicle-stimulating hormone receptor (FSHR) -29 and Ser680Asn polymorphisms in IVF/ICSI
- Diagnostic value of the candidate microRNAs in thyroid fine-needle aspiration biopsy (FNAB) samples
- Investigation of the role of β-TrCP in growth hormone transduction defect (GHTD)
- High-intensity interval training with long duration intervals is more effective than short duration intervals for improving glycolytic capacity in the rats’ gastrocnemius muscle
- The effects of circuit resistance training on plasma progranulin level, insulin resistance and body composition in obese men
- Sexual motivation: problem solved and new problems introduced
- Case Reports
- Ovarian and uterine leiomyosarcoma: which one is the primary?
- Uterine-conserving approach in ruptured intramyometrial ectopic pregnancy