High-intensity interval training with long duration intervals is more effective than short duration intervals for improving glycolytic capacity in the rats’ gastrocnemius muscle

Azadeh Akmali; Marziyeh Saghebjoo

doi:10.1515/hmbci-2019-0035

Artikel

High-intensity interval training with long duration intervals is more effective than short duration intervals for improving glycolytic capacity in the rats’ gastrocnemius muscle

Azadeh Akmali und Marziyeh Saghebjoo

Veröffentlicht/Copyright: 19. Oktober 2019

Veröffentlicht von

Veröffentlichen auch Sie bei De Gruyter Brill

Manuskript einreichen Informationen für Autor*innen

Aus der Zeitschrift Hormone Molecular Biology and Clinical Investigation Band 41 Heft 2

Abstract

Background

There is little data regarding the ability of high-intensity interval training (HIIT) to increase of glycolytic capacity and intramuscular metabolic adaptations. The goal of this study was to evaluate the effects of HIIT (8 weeks, 5 times/week) with short (HIIT_{1 min}: 16 × 1 min work and active recovery at 80–95% and 50–60% VO₂max, respectively) and long (HIIT_{4 min}: 4 × 4 min work and active recovery at 80–95% and 50–60% VO₂max, respectively) duration intervals and 4 weeks detraining on the levels of phosphofructokinase (PFK), glycogen synthase 1 (GYS1), monocarboxylate transporter 4 (MCT4) and lactate dehydrogenase (LDH) activity in the rats’ gastrocnemius muscle.

Materials and methods

Fifty-four male Wistar rats were assigned into three groups, including HIIT_{1 min}, HIIT_{4 min} and control (Ctrl). After 48 h of the last training session and after 4 weeks of detraining, the rats were sacrificed, and the gastrocnemius muscles were isolated.

Results

The PFK levels in the HIIT_{4 min} group was significantly higher than in the HIIT_{1 min} and Ctrl groups, and after the detraining period in the HIIT_{4 min}DT group significantly decreased compared to the HIIT_{4 min} group. The LDH activity in the HIIT_{4 min} and HIIT_{1 min} groups were significantly higher than the Ctrl group and the increasing trend in the HIIT_{4 min} group was more than the HIIT_{1 min} group. There was no significant change in LDH activity after detraining compared to training. No significant changes were observed in the level of GYS1 and MCT4 after HIIT.

Conclusions

Eight weeks of HIIT with long duration intervals induced more improvements in intramuscular glycolytic capacity than a short duration. After short-term detraining, some of these adaptations have remained.

Keywords: glycogen synthase 1; high-intensity interval training; lactate dehydrogenase; monocarboxylate transporter protein 4; phosphofructokinase

Acknowledgments

We would like to thank Dr. Hedayati, the Head of Cellular and Molecular Endocrine Research Center at Shahid Beheshti University of Medical Sciences for assisting in the laboratory analysis.

Author Statement

Research funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Conflict of interest: None of the authors have financial or other conflicts of interest.
Informed consent: Not applicable.
Ethical approval: All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

References

[1] Cassidy S, Thoma C, Houghton D, Trenell MI. High-intensity interval training: a review of its impact on glucose control and cardiometabolic health. Diabetologia. 2017;60:7–23.10.1007/s00125-016-4106-1Suche in Google Scholar PubMed PubMed Central

[2] Horii N, Hasegawa N, Fujie S, Uchida M, Miyamoto-Mikami E, Hashimoto T, et al. High-intensity intermittent exercise training with chlorella intake accelerates exercise performance and muscle glycolytic and oxidative capacity in rats. Am J Physiol Regul Integr Comp Physiol. 2017;312:R520–8.10.1152/ajpregu.00383.2016Suche in Google Scholar PubMed

[3] Hoshino D, Kitaoka Y, Hatta H. High-intensity interval training enhances oxidative capacity and substrate availability in skeletal muscle. J Phys Fit Sports Med. 2016;5:13–23.10.7600/jpfsm.5.13Suche in Google Scholar

[4] Nalbandian M, Takeda M. Lactate as a signaling molecule that regulates exercise-induced adaptations. Biology. 2016;5:38.10.3390/biology5040038Suche in Google Scholar PubMed PubMed Central

[5] Carnevali Jr LC, Eder R, Lira FS, Lima WP, Gonçalves DC, Zanchi NE, et al. Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats. Braz J Med Biol Res. 2012;45:777–83.10.1590/S0100-879X2012007500105Suche in Google Scholar

[6] Goff M, Chen G. Progresses of the past decade on factors contributing to skeletal muscle glycogen synthase regulation. Metabolomics. 2016;6:174.10.4172/2153-0769.1000174Suche in Google Scholar

[7] Manabe Y, Gollisch KS, Holton L, Kim YB, Brandauer J, Fujii NL, et al. Exercise training-induced adaptations associated with increases in skeletal muscle glycogen content. FEBS J. 2013;280:916–26.10.1111/febs.12085Suche in Google Scholar PubMed PubMed Central

[8] Han S, Lee H, Kim H, Kim D, Choi C, Park J. A 6-week sprint interval training program changes anaerobic power, quadriceps moment, and subcutaneous tissue thickness. Int J Sports Med. 2017;38:105–10.10.1055/s-0042-111437Suche in Google Scholar PubMed

[9] Adeva-Andany MM, González-Lucán M, Donapetry-García C, Fernández-Fernández C, Ameneiros-Rodríguez E. Glycogen metabolism in humans. BBA Clin. 2016;5:85–100.10.1016/j.bbacli.2016.02.001Suche in Google Scholar PubMed PubMed Central

[10] Abe T, Kitaoka Y, Kikuchi DM, Takeda K, Numata O, Takemasa T. High-intensity interval training-induced metabolic adaptation coupled with an increase in Hif-1α and glycolytic protein expression. J Appl Physiol (1985). 2015;119:1297–302.10.1152/japplphysiol.00499.2015Suche in Google Scholar PubMed

[11] Bishop D, Edge J, Thomas C, Mercier J. Effects of high-intensity training on muscle lactate transporters and postexercise recovery of muscle lactate and hydrogen ions in women. Am J Physiol Regul Integr Comp Physiol. 2008;295:R1991–8.10.1152/ajpregu.00863.2007Suche in Google Scholar PubMed

[12] Bishop D, Edge J, Thomas C, Mercier J. High-intensity exercise acutely decreases the membrane content of MCT1 and MCT4 and buffer capacity in human skeletal muscle. J Appl Physiol. 2007;102:616–21.10.1152/japplphysiol.00590.2006Suche in Google Scholar PubMed

[13] Buchheit M, Laursen PB. High-intensity interval training, solutions to the program ming puzzle. Sports Med. 2013;43:313–38.10.1007/s40279-013-0029-xSuche in Google Scholar PubMed

[14] Vermeulen R, Plancke L, Boshuizen B, de Bruijn M, Delesalle C. Effects of training on equine muscle physiology and muscle adaptations in response to different training approaches. Vlaams Diergeneeskundig Tijdschrift. 2017;86:224–30.10.21825/vdt.v86i4.16183Suche in Google Scholar

[15] Cipryan L, Laursen PB, Plews DJ. Cardiac autonomic response following high-intensity running work-to-rest interval manipulation. Eur J Sports Sci. 2016;16:808–17.10.1080/17461391.2015.1103317Suche in Google Scholar PubMed

[16] Buchheit M, Laursen PB. High-intensity interval training, solutions to the programming puzzle Part 2 (Anaerobic energy and neuromuscular load). Sports Med. 2013;43:927–54.10.1007/s40279-013-0066-5Suche in Google Scholar PubMed

[17] Germano MD, Sindorf MA, da Silva CE, Evangelista AL, Bocalini DS, Lopes CR. High intensity interval training: cardiorespiratory adaptations, metabolic and performance. Int J Sports Sci. 2015;5:240–7.Suche in Google Scholar

[18] Hwang PS, Andre TL, McKinley-Barnard SK, Marroquín FE, Gann JJ, Song JJ, et al. Resistance training–induced elevations in muscular strength in trained men are maintained after 2 weeks of detraining and not differentially affected by whey protein supplementation. J Strength Cond Res. 2017;31:869–81.10.1519/JSC.0000000000001807Suche in Google Scholar PubMed

[19] Lee S, Leone TC, Rogosa L, Rumsey J, Ayala J, Coen PM, et al. Skeletal muscle PGC-1β signaling is sufficient to drive an endurance exercise phenotype and to counteract components of detraining in mice. Am J Physiol Endocrinol Metab. 2017;312:E394–406.10.1152/ajpendo.00380.2016Suche in Google Scholar PubMed PubMed Central

[20] Afzalpour ME, Chadorneshin HT, Foadoddini M, Eivari HA. Comparing interval and continuous exercise training regimens on neurotrophic factors in rat brain. Physiol Behav. 2015;147:78–83.10.1016/j.physbeh.2015.04.012Suche in Google Scholar PubMed

[21] Borzykh AA, Kuzmin IV, Mart’ianov AA, Borovik AS, Sharova AP, Tarasova OS, et al. Changes of rat respiratory and locomotory muscles during aerobic exercise training in continuous and interval regimens. Biofizika. 2012;57:880–7.10.1134/S0006350912050053Suche in Google Scholar

[22] Tucker WJ, Sawyer BJ, Jarrett CL, Bhammar DM, Gaesser GA. Physiological responses to high-intensity interval exercise differing in interval duration. J Strength Cond Res. 2015;29:3326–35.10.1519/JSC.0000000000001000Suche in Google Scholar PubMed

[23] Kessler HS, Sisson SB, Short KR. The potential for high-intensity interval training to reduce cardiometabolic disease risk. Sports Med. 2012;42:489–509.10.2165/11630910-000000000-00000Suche in Google Scholar PubMed

[24] Lu K, Wang L, Wang C, Yang Y, Hu D, Ding R. Effects of high-intensity interval versus continuous moderate-intensity aerobic exercise on apoptosis, oxidative stress and metabolism of the infarcted myocardium in a rat model. Mol Med Rep. 2015;12:2374–82.10.3892/mmr.2015.3669Suche in Google Scholar PubMed

[25] Phillips A, Cobbold CA. Comparison of the effects of aerobic and intense exercise on the type 2 diabetes mellitus risk marker adipokines, adiponectin and retinol binding protein-4. Int J Chronic Dis. 2014;2014:358058.10.1155/2014/358058Suche in Google Scholar PubMed PubMed Central

[26] MacDougall JD, Hicks AL, MacDonald JR, McKelvie RS, Green HJ, Smith KM. Muscle performance and enzymatic adaptations to sprint interval training. J Appl Physiol. 1998;84:2138–42.10.1152/jappl.1998.84.6.2138Suche in Google Scholar PubMed

[27] Roberts AD, Billeter R, Howald H. Anaerobic muscle enzyme changes after interval training. Int J Sports Med. 1982;3:18–21.10.1055/s-2008-1026055Suche in Google Scholar PubMed

[28] Rodas G, Ventura JL, Cadefau JA, Cussó R, Parra JA. Short training programme for the rapid improvement of both aerobic and anaerobic metabolism. Eur J Appl Physiol. 2000;82:480–6.10.1007/s004210000223Suche in Google Scholar PubMed

[29] Batra A, Zatoń M. Effect of high-intensity interval training on anaerobic capacity in ITF taekwondo practitioners. J Combat Sports Martial Arts. 2017;8:61–6.10.5604/01.3001.0010.5238Suche in Google Scholar

[30] Kohn TA, Essen-Gustavsson B, Myburgh KH. Specific muscle adaptations in type II fibers after high-intensity interval training of well-trained runners. Scand J Med Sci Sports. 2011;21:765–72.10.1111/j.1600-0838.2010.01136.xSuche in Google Scholar PubMed

[31] Nielsen JN, Derave W, Kristiansen S, Ralston E, Ploug T, Richter EA. Glycogen synthase localization and activity in rat skeletal muscle is strongly dependent on glycogen content. J Physiol. 2001;531:757–69.10.1111/j.1469-7793.2001.0757h.xSuche in Google Scholar PubMed PubMed Central

[32] Laursen PB, Jenkins DG. The scientific basis for high-intensity interval training. Sports Med. 2002;32:53–73.10.2165/00007256-200232010-00003Suche in Google Scholar PubMed

[33] Fournier M, Ricci J, Taylor AW, Ferguson RJ, Montpetit RR, Chaitman BR. Skeletal muscle adaptation in adolescent boys: sprint and endurance training and detraining. Med Sci Sports Exerc. 1982;14:453–6.10.1249/00005768-198206000-00008Suche in Google Scholar PubMed

[34] Puype J, Van Proeyen K, Raymackers JM, Deldicque L, Hespel P. Sprint interval training in hypoxia stimulates glycolytic enzyme activity. Med Sci Sports Exerc. 2013;45:2166–74.10.1249/MSS.0b013e31829734aeSuche in Google Scholar PubMed

[35] Kelley KM, Hamann JJ, Navarre C, Gladden LB. Lactate metabolism in resting and contracting canine skeletal muscle with elevated lactate concentration. J Appl Physiol (1985). 2002;93:865–72.10.1152/japplphysiol.01119.2001Suche in Google Scholar PubMed

[36] McGinley C, Bishop DJ. Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men. J Appl Physiol. 2016;121:1290–305.10.1152/japplphysiol.00630.2016Suche in Google Scholar PubMed

[37] Thomas C, Bishop DJ, Lambert K, Mercier J, Brooks GA. Effects of acute and chronic exercise on sarcolemmal MCT1 and MCT4 contents in human skeletal muscles: current status. Am J Physiol Regul Integr Comp Physiol. 2012;302:R1.10.1152/ajpregu.00250.2011Suche in Google Scholar PubMed

[38] McGinley C, Bishop DJ. Rest interval duration does not influence adaptations in acid/base transport proteins following 10 wk of sprint-interval training in active women. Am J Physiol Regul Integr Comp Physiol. 2017;312:R702–17.10.1152/ajpregu.00459.2016Suche in Google Scholar PubMed

[39] McGinley C, Bishop DJ. Distinct protein and mRNA kinetics of skeletal muscle proton transporters following exercise can influence interpretation of adaptations to training. Exp Physiol. 2016;101:1565–80.10.1113/EP085921Suche in Google Scholar PubMed

Received: 2019-06-27

Accepted: 2019-09-09

Published Online: 2019-10-19

Sie haben derzeit keinen Zugang zu diesem Inhalt.

Artikel in diesem Heft

https://doi.org/10.1515/hmbci-2019-0035

Schlagwörter für diesen Artikel

glycogen synthase 1; high-intensity interval training; lactate dehydrogenase; monocarboxylate transporter protein 4; phosphofructokinase