Diagnostic value of the candidate microRNAs in thyroid fine-needle aspiration biopsy (FNAB) samples
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Mohammad-Reza Mahmoudian-Sani
Abstract
Background
Thyroid cancer (TC) is known to be the most common endocrine malignancy with an incidence rate which has increased by 2.3-fold over the past 30 years. Approximately, 30% of the thyroid fine-needle aspiration biopsy (FNAB) outcomes are indecisive. Moreover, researchers recognized multiple differentially expressed microRNAs (miRNAs) as candidate diagnostic markers for thyroid nodules. The purpose of this study was to identify thyroid tumor-associated miRNAs in FNAB with the capacity to be developed as unique biomarkers.
Materials and methods
According to the study design, a quantitative real time reverse transcription polymerase chain reaction (qRT-PCR) was applied to evaluate the expression levels of nine miRNAs (Let7, miR-34a, miR-146b, miR-221, miR-151, miR-155, miR-181b, miR-222 and miR-375) among 224 FNA samples as the training set.
Results
The findings of this study revealed that miR-181b and miR-146b are the best predictors to diagnose benign thyroid FNA samples from malignant samples. However, the remaining miRNAs were co-expressed and had no significant effect on the predictor model. On the other hand, sensitivity and specificity of miR-181b and miR-146b were reported at 83.0%–83.0% and 83.0%–66.0%, respectively.
Conclusions
According to the results of this study, miR-146b and miR-181b might be considered as adjunct markers contributing to thyroid FNAB in tumor types. In addition, miR-146b and miR-181b were recognized as biomarkers for discriminating benign thyroid nodules from malignant ones. It is suggested that further prospective clinical trials be conducted to evaluate the accuracy of such findings in a larger cohort and determine the clinical uses.
Author statement
Research funding: The present research has been supported through an award from Shahrekord University of Medical Sciences, Shahrekord, Iran (grant No. 2351).
Conflict of interest: Hereby, it is notified that there are no conflicts of interests.
Informed consent: Written informed consents were obtained.
Ethical approval: This research was approved by the Ethics Committee of Shahrekord University of Medical Sciences with the Ethics Code: IR.SKUMS.REC.1396.1.
References
[1] Sajid-Crokett S, Hershman J. Thyroid nodules and cancer in the elderly. 2015. De Groot LJ, Chrousos G, Dungan K, и сарадници. Endotext [Internet]. South Dartmouth (MA): MDText.com, Inc.; 2000.Suche in Google Scholar
[2] Grande E, Díez JJ, Zafon C, Capdevila J. Thyroid cancer: molecular aspects and new therapeutic strategies. J Thyroid Res. 2012;2012:10.10.1155/2012/847108Suche in Google Scholar PubMed PubMed Central
[3] McCabe C. Novel molecular markers in thyroid cancer. In: 196th Meeting of the Society for Endocrinology and Society for Endocrinology joint Endocrinology and Diabetes Day. BioScientifica; 10:2005.Suche in Google Scholar
[4] Singer PA, Cooper DS, Daniels GH, Ladenson PW, Greenspan FS, Levy EG, et al. Treatment guidelines for patients with thyroid nodules and well-differentiated thyroid cancer. Arch Intern Med. 1996;156:2165–72.10.1001/archinte.1996.00440180017002Suche in Google Scholar
[5] Weber F, Shen L, Aldred MA, Morrison CD, Frilling A, Saji M, et al. Genetic classification of benign and malignant thyroid follicular neoplasia based on a three-gene combination. J Clin Endocrinol Metab. 2005;90:2512–21.10.1210/jc.2004-2028Suche in Google Scholar PubMed
[6] Grant CS. Recurrence of papillary thyroid cancer after optimized surgery. Gland Surg. 2015;4:52–62.Suche in Google Scholar
[7] Lee JH, Lee ES, Kim YS. Clinicopathologic significance of BRAF V600E mutation in papillary carcinomas of the thyroid. Cancer. 2007;110:38–46.10.1002/cncr.22754Suche in Google Scholar PubMed
[8] Shibru D, Hwang J, Khanafshar E, Duh QY, Clark OH, Kebebew E. Does the 3-gene diagnostic assay accurately distinguish benign from malignant thyroid neoplasms? Cancer. 2008;113:930–5.10.1002/cncr.23703Suche in Google Scholar PubMed
[9] Kebebew E, Peng M, Reiff E, Duh Q-Y, Clark OH, McMillan A. Diagnostic and prognostic value of angiogenesis-modulating genes in malignant thyroid neoplasms. Surgery. 2005;138:1102–10.10.1016/j.surg.2005.05.025Suche in Google Scholar PubMed
[10] Hoque MO, Rosenbaum E, Westra WH, Xing M, Ladenson P, Zeiger MA, et al. Quantitative assessment of promoter methylation profiles in thyroid neoplasms. J Clin Endocrinol Metab. 2005;90:4011–8.10.1210/jc.2005-0313Suche in Google Scholar PubMed
[11] Chung KW, Yang SK, Lee GK, Kim EY, Kwon S, Lee SH, et al. Detection of BRAFV600E mutation on fine needle aspiration specimens of thyroid nodule refines cyto-pathology diagnosis, especially in BRAFV600E mutation-prevalent area. Clin Endocrinol. 2006;65:660–6.10.1111/j.1365-2265.2006.02646.xSuche in Google Scholar PubMed
[12] Rowe LR, Bentz BG, Bentz JS. Utility of BRAF V600E mutation detection in cytologically indeterminate thyroid nodules. Cytojournal. 2006;3:10.10.1186/1742-6413-3-10Suche in Google Scholar PubMed PubMed Central
[13] Ma W, Zhao X, Liang L, Wang G, Li Y, Miao X, et al. miR-146a and miR-146b promote proliferation, migration and invasion of follicular thyroid carcinoma via inhibition of ST8SIA4. Oncotarget. 2017;8:28028.10.18632/oncotarget.15885Suche in Google Scholar PubMed PubMed Central
[14] Li D, Jian W, Wei C, Song H, Gu Y, Luo Y, et al. Down-regulation of miR-181b promotes apoptosis by targeting CYLD in thyroid papillary cancer. Int J Clin Exp Pathol. 2014;7:7672–80.Suche in Google Scholar
[15] Zhang X, Li M, Zuo K, Li D, Ye M, Ding L, et al. Upregulated miR-155 in papillary thyroid carcinoma promotes tumor growth by targeting APC and activating Wnt/β-catenin signaling. J Clin Endocrinol Metab. 2013;98:E1305–13.10.1210/jc.2012-3602Suche in Google Scholar PubMed
[16] Huang Y, Yu S, Cao S, Yin Y, Hong S, Guan H, et al. MicroRNA-222 promotes invasion and metastasis of papillary thyroid cancer through targeting protein phosphatase 2 regulatory subunit B alpha expression. Thyroid. 2018;28:1162–73.10.1089/thy.2017.0665Suche in Google Scholar PubMed
[17] Marques JC, Fuziwara CS, Yamashita AS, Rezende E, da-Silva MJ, Kimura ET. Effects of let-7 microRNA on cell growth and differentiation of papillary thyroid cancer. Transl Oncol. 2009;2:236–41.10.1593/tlo.09151Suche in Google Scholar PubMed PubMed Central
[18] Ma Y, Qin H, Cui Y. MiR-34a targets GAS1 to promote cell proliferation and inhibit apoptosis in papillary thyroid carcinoma via PI3K/Akt/Bad pathway. Biochem Biophys Res Commun. 2013;441:958–63.10.1016/j.bbrc.2013.11.010Suche in Google Scholar PubMed
[19] Lima CR, Geraldo MV, Fuziwara CS, Kimura ET, Santos MF. MiRNA-146b-5p upregulates migration and invasion of different Papillary Thyroid Carcinoma cells. BMC Cancer. 2016;16:108.10.1186/s12885-016-2146-zSuche in Google Scholar PubMed PubMed Central
[20] Wang X-Z, Hang Y-K, Liu J-B, Hou Y-Q, Wang N, Wang M-J. Over-expression of microRNA-375 inhibits papillary thyroid carcinoma cell proliferation and induces cell apoptosis by targeting ERBB2. J Pharmacol Sci. 2016;130:78–84.10.1016/j.jphs.2015.12.001Suche in Google Scholar PubMed
[21] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402–8.10.1006/meth.2001.1262Suche in Google Scholar PubMed
[22] Panebianco F, Mazzanti C, Tomei S, Aretini P, Franceschi S, Lessi F, et al. The combination of four molecular markers improves thyroid cancer cytologic diagnosis and patient management. BMC Cancer. 2015;15:918.10.1186/s12885-015-1917-2Suche in Google Scholar PubMed PubMed Central
[23] Shen R, Liyanarachchi S, Li W, Wakely Jr PE, Saji M, Huang J, et al. MicroRNA signature in thyroid fine needle aspiration cytology applied to “atypia of undetermined significance” cases. Thyroid. 2012;22:9–16.10.1089/thy.2011.0081Suche in Google Scholar PubMed PubMed Central
[24] Mazeh H, Levy Y, Mizrahi I, Appelbaum L, Ilyayev N, Halle D, et al. Differentiating benign from malignant thyroid nodules using micro ribonucleic acid amplification in residual cells obtained by fine needle aspiration biopsy. J Surg Res. 2013;180:216–21.10.1016/j.jss.2012.04.051Suche in Google Scholar PubMed
[25] Lee YS, Lim YS, Lee JC, Wang SG, Park HY, Kim SY, et al. Differential expression levels of plasma-derived miR-146b and miR-155 in papillary thyroid cancer. Oral Oncol. 2015;51:77–83.10.1016/j.oraloncology.2014.10.006Suche in Google Scholar PubMed
[26] Yu S, Liu Y, Wang J, Guo Z, Zhang Q, Yu F, et al. Circulating microRNA profiles as potential biomarkers for diagnosis of papillary thyroid carcinoma. J Clin Endocrinol Metab. 2012;97:2084–92.10.1210/jc.2011-3059Suche in Google Scholar PubMed
[27] Lee JC, Zhao JT, Gundara J, Serpell J, Bach LA, Sidhu S. Papillary thyroid cancer-derived exosomes contain miRNA-146b and miRNA-222. J Surg Res. 2015;196:39–48.10.1016/j.jss.2015.02.027Suche in Google Scholar PubMed
[28] Mazeh H, Deutch T, Karas A, Bogardus KA, Mizrahi I, Gur-Wahnon D, et al. Next-generation sequencing identifies a highly accurate miRNA panel that distinguishes well-differentiated thyroid cancer from benign thyroid nodules. Cancer Epidemiol Biomarkers Prev. 2018;27:858–63.10.1158/1055-9965.EPI-18-0055Suche in Google Scholar PubMed
[29] Rossi ED, Bizzarro T, Martini M, Straccia P, Lombardi CP, Pontecorvi A, et al. The role of fine-needle aspiration in the thyroid nodules of elderly patients. Oncotarget. 2016;7:11850–9.10.18632/oncotarget.7643Suche in Google Scholar PubMed PubMed Central
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Artikel in diesem Heft
- Letters to the Editor
- Androgen-driven COVID-19 infection – is testosterone an enemy or a friend?
- Tumor necrosis factor-α gene-1031T/C promoter polymorphism and endometriosis
- Original Articles
- Serum FGF-21 and FGF-23 in association with gestational diabetes: a longitudinal case-control study
- Neutrophil and monocyte ratios to high-density lipoprotein-cholesterol and adiponectin as biomarkers of nascent metabolic syndrome
- Predicting long-term cardiovascular outcomes of patients with acute myocardial infarction using soluble ST2
- Anti-inflammatory activity of emu oil-based nanofibrous scaffold through downregulation of IL-1, IL-6, and TNF-α pro-inflammatory cytokines
- A comparative study of the antidiabetic effect of two training protocols in streptozotocin-nicotinamide diabetic rats
- Gonadotrophins modulate cell death-related genes expression in human endometrium
- Exploring the activity of the enzyme 11β-hydroxylase in the polycystic ovary syndrome
- The effects of follicle-stimulating hormone receptor (FSHR) -29 and Ser680Asn polymorphisms in IVF/ICSI
- Diagnostic value of the candidate microRNAs in thyroid fine-needle aspiration biopsy (FNAB) samples
- Investigation of the role of β-TrCP in growth hormone transduction defect (GHTD)
- High-intensity interval training with long duration intervals is more effective than short duration intervals for improving glycolytic capacity in the rats’ gastrocnemius muscle
- The effects of circuit resistance training on plasma progranulin level, insulin resistance and body composition in obese men
- Sexual motivation: problem solved and new problems introduced
- Case Reports
- Ovarian and uterine leiomyosarcoma: which one is the primary?
- Uterine-conserving approach in ruptured intramyometrial ectopic pregnancy
