Startseite Overview of dengue diagnostic limitations and potential strategies for improvement
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Overview of dengue diagnostic limitations and potential strategies for improvement

  • Aruna Devi Selvaraj , Anand Ramaian Santhaseela ORCID logo und Elavarasan Tamilmani ORCID logo EMAIL logo
Veröffentlicht/Copyright: 29. Januar 2025
Diagnosis
Aus der Zeitschrift Diagnosis Band 12 Heft 3

Abstract

Introduction

Dengue is a viral infection caused by any one of the four related dengue virus (DENV) serotypes, 1–4. DENV is a single-stranded RNA virus belonging to the genus Flavivirus. Dengue can cause a range of symptoms, from mild to severe life-threatening illness. Currently, treatment for DENV is limited to supportive care, with better outcomes achieved through early diagnosis. The WHO has suggested that dengue mortality can be reduced to nearly zero by implementing appropriate clinical management strategies, such as early laboratory diagnosis. This calls for diagnostic approaches that combine high sensitivity and specificity, while also being suitable for point-of-care testing (POCT) in remote locations with minimal staff training and low testing costs.

Content

In this paper, we outline the limitations of existing confirmatory dengue diagnostic methods, such as ELISA and RT-PCR, which are time-consuming, expensive, and require skilled personnel. We also highlight alternative strategies to overcome these challenges. Additionally, the paper emphasizes the growing clinical demand for diagnosing severe dengue to reduce the risk of death, which must be addressed by next-generation dengue diagnostic approaches.

Summary

We propose the adoption of alternative strategies, such as fluorescence immunoassay (FIA) and chemiluminescence immunoassay (CLIA), which have the potential to overcome the limitations of existing dengue diagnostic methods.

Outlook

Improvements in dengue diagnosis, with a specific focus on identifying severe dengue in POCT setting, can help achieve the goal of zero deaths from dengue.

Keywords: dengue; diagnosis; RT-PCR; ELISA; CLIA; FIA
Corresponding author: Elavarasan Tamilmani, Chief Technology Officer, Matrix Labs Private Limited, Chennai 600077, Tamil Nadu, India, E-mail:

  1. Research ethics: Not applicable.

  2. Informed consent: Not applicable.

  3. Author contributions: E.T. and A.D.S carried out the conception, design and drafting of the paper. A.R.S carried out revising the manuscript with inputs from E.T. and A.D.S. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  4. Use of Large Language Models, AI and Machine Learning Tools: None declared.

  5. Conflict of interest: The authors state no conflict of interest.

  6. Research funding: None declared.

  7. Data availability: Not applicable.

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Received: 2024-10-23
Accepted: 2024-12-26
Published Online: 2025-01-29

© 2025 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Accuracy of pulse wave velocity for screening coronary artery disease: a systematic review and meta-analysis
  4. Prenatal screening for genetic disorders: updated guidelines, proposed counseling, a holistic approach for primary health care providers in developing countries
  5. A scoping review of fever of unknown origin with normal serum C-reactive protein
  6. Mini Review
  7. Overview of dengue diagnostic limitations and potential strategies for improvement
  8. Opinion Papers
  9. Demystifying cognitive bias in the diagnostic process for frontline clinicians and educators; new words for old ideas
  10. Physicians’ prism: illuminating history with structured expertise
  11. Original Articles
  12. Implementation of a curriculum on communicating diagnostic uncertainty for clerkship-level medical students: a pseudorandomized and controlled study
  13. A synthesized differential diagnosis is associated with fewer diagnostic errors compared to an inventorial list
  14. Equity-Driven Diagnostic Excellence framework: An upstream approach to minimize risk of diagnostic inequity
  15. Two decades of autopsy-detected diagnostic errors in Japan
  16. Does management reasoning display context specificity? An exploration of sleep loss and other distracting situational (contextual) factors in clinical reasoning
  17. Cognitive biases in osteopathic diagnosis: a mixed study among French osteopaths
  18. Racial and ethnic disparities in pediatric emergency department patients with missed opportunities for diagnostic excellence
  19. Diagnostic accuracy of non-mydriatic fundus photography as a triage and telemedicine tool for patients with vision loss
  20. “Innumerable” lesion burden on brain MRI ‒ a diagnostic approach
  21. Factors associated with positive findings of deep infection on computed tomography among patients with extremity cellulitis
  22. Exploring emergency department providers’ uncertainty in neurological clinical reasoning
  23. Analytical performance and user-friendliness of four point-of-care measuring systems for monitoring prothrombin time international normalized ratio in the hands of the intended users
  24. Fetal hematological phenotypes of various hemoglobinopathies and demonstration of embryonic hemoglobins on capillary electrophoresis: a large cohort data from prenatal screening program
  25. Development and assessment of autoverification system for routine coagulation assays in inpatient and outpatient settings of tertiary care hospital: algorithm performance and impact on laboratory efficiency
  26. Validation of new, circulating biomarkers for gliomas
  27. Short Communications
  28. Using language to evaluate curricular impact: a novel approach in assessing clinical reasoning curricula
  29. Comparative evaluation of routine coagulation testing on Stago sthemO 301 and Werfen ACL TOP 750
  30. Letters to the Editor
  31. Reversible systemic vasoconstriction syndrome: a new diagnostic family of generalized vasospasm in multiple organs
  32. The value of designating symptoms as “vague” in diagnosis
  33. Immunoglobulin E in an inverted skin-prick test for rapid detection of cutaneous antigens
https://doi.org/10.1515/dx-2024-0173
Schlagwörter für diesen Artikel
dengue; diagnosis; RT-PCR; ELISA; CLIA; FIA

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Accuracy of pulse wave velocity for screening coronary artery disease: a systematic review and meta-analysis
  4. Prenatal screening for genetic disorders: updated guidelines, proposed counseling, a holistic approach for primary health care providers in developing countries
  5. A scoping review of fever of unknown origin with normal serum C-reactive protein
  6. Mini Review
  7. Overview of dengue diagnostic limitations and potential strategies for improvement
  8. Opinion Papers
  9. Demystifying cognitive bias in the diagnostic process for frontline clinicians and educators; new words for old ideas
  10. Physicians’ prism: illuminating history with structured expertise
  11. Original Articles
  12. Implementation of a curriculum on communicating diagnostic uncertainty for clerkship-level medical students: a pseudorandomized and controlled study
  13. A synthesized differential diagnosis is associated with fewer diagnostic errors compared to an inventorial list
  14. Equity-Driven Diagnostic Excellence framework: An upstream approach to minimize risk of diagnostic inequity
  15. Two decades of autopsy-detected diagnostic errors in Japan
  16. Does management reasoning display context specificity? An exploration of sleep loss and other distracting situational (contextual) factors in clinical reasoning
  17. Cognitive biases in osteopathic diagnosis: a mixed study among French osteopaths
  18. Racial and ethnic disparities in pediatric emergency department patients with missed opportunities for diagnostic excellence
  19. Diagnostic accuracy of non-mydriatic fundus photography as a triage and telemedicine tool for patients with vision loss
  20. “Innumerable” lesion burden on brain MRI ‒ a diagnostic approach
  21. Factors associated with positive findings of deep infection on computed tomography among patients with extremity cellulitis
  22. Exploring emergency department providers’ uncertainty in neurological clinical reasoning
  23. Analytical performance and user-friendliness of four point-of-care measuring systems for monitoring prothrombin time international normalized ratio in the hands of the intended users
  24. Fetal hematological phenotypes of various hemoglobinopathies and demonstration of embryonic hemoglobins on capillary electrophoresis: a large cohort data from prenatal screening program
  25. Development and assessment of autoverification system for routine coagulation assays in inpatient and outpatient settings of tertiary care hospital: algorithm performance and impact on laboratory efficiency
  26. Validation of new, circulating biomarkers for gliomas
  27. Short Communications
  28. Using language to evaluate curricular impact: a novel approach in assessing clinical reasoning curricula
  29. Comparative evaluation of routine coagulation testing on Stago sthemO 301 and Werfen ACL TOP 750
  30. Letters to the Editor
  31. Reversible systemic vasoconstriction syndrome: a new diagnostic family of generalized vasospasm in multiple organs
  32. The value of designating symptoms as “vague” in diagnosis
  33. Immunoglobulin E in an inverted skin-prick test for rapid detection of cutaneous antigens
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