The cloning, expression and purification of recombinant human neuritin from Escherichia coli and the partial analysis of its neurobiological activity
-
Yuanyuan Li
and
Lei Yang
Abstract
Neuritin (Nrn1) is a neurotrophic factor that plays various roles in neural development and synaptic plasticity. In this study, the NRN1 gene was cloned and expressed in Escherichia coli and then recombinant neuritin protein was purified so that its neurobiological activity could be evaluated. The protein, which was obtained at a concentration of 0.45 mg/ml and > 90% purity, had the predicted molecular weight of 30 kDa, as determined via sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Western blot analysis confirmed that an anti-neuritin antibody could recognize the fusion protein. Subsequent functional analyses revealed that recombinant neuritin promoted neurite outgrowth in embryonic chicken dorsal root ganglia and PC12 cells. These results suggest that recombinant neuritin protein could be a valuable tool for inducing neurite regeneration, for instance in cases of spinal cord injury or neurological diseases.
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Articles in the same Issue
- How taste works: cells, receptors and gustatory perception
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- Somatic stem cell aging and malignant transformation – impact on therapeutic application
- 1H NMR-based metabolic profiling for evaluating poppy seed rancidity and brewing
- Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: size does not matter; quality control of targeted clones does
- Death domain associated protein (Daxx), a multi-functional protein
- In silico screening of alleged miRNAs associated with cell competition: an emerging cellular event in cancer
- MGL induces nuclear translocation of EndoG and AIF in caspase-independent T cell death
- Cerivastatin represses atherogenic gene expression through the induction of KLF2 via isoprenoid metabolic pathways
- Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells
- Purinergic signaling and the functioning of the nervous system cells
- The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes
- Ramipril inhibits high glucose-stimulated up-regulation of adhesion molecules via the ERK1/2 MAPK signaling pathway in human umbilical vein endothelial cells
- The effect of nicotine on the expressions of the α7 nicotinic receptor gene and Bax and Bcl-2 proteins in the mammary gland epithelial-7 breast cancer cell line and its relationship to drug resistance
- The cloning, expression and purification of recombinant human neuritin from Escherichia coli and the partial analysis of its neurobiological activity
