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Non-cooperative immobilization of residual water bound in lyophilized photosynthetic lamellae

  • Hubert Harańczyk EMAIL logo , Ewelina Baran , Piotr Nowak , Małgorzata Florek-Wojciechowska , Anna Leja , Dorota Zalitacz and Kazimierz Strzałka
Published/Copyright: March 5, 2016
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Cellular and Molecular Biology Letters
From the journal Cellular and Molecular Biology Letters Volume 20 Issue 5

Abstract

This study applied 1H-NMR in time and in frequency domain measurements to monitor the changes that occur in bound water dynamics at decreased temperature and with increased hydration level in lyophilizates of native wheat photosynthetic lamellae and in photosynthetic lamellae reconstituted from lyophilizate. Proton relaxometry (measured as free induction decay = FID) distinguishes a Gaussian component S within the NMR signal (o). This comes from protons of the solid matrix of the lamellae and consists of (i) an exponentially decaying contribution L1 from mobile membrane protons, presumably from lipids, and from water that is tightly bound to the membrane surface and thus restricted in mobility; and (ii) an exponentially decaying component L2 from more mobile, loosely bound water pool. Both proton relaxometry data and proton spectroscopy show that dry lyophilizate incubated in dry air, i.e., at a relative humidity (p/p0) of 0% reveals a relatively high hydration level. The observed liquid signal most likely originates from mobile membrane protons and a tightly bound water fraction that is sealed in pores of dry lyophilizate and thus restricted in mobility. The estimations suggest that the amount of sealed water does not exceed the value characteristic for the main hydration shell of a phospholipid. Proton spectra collected for dry lyophilizate of photosynthetic lamellae show a continuous decrease in the liquid signal component without a distinct freezing transition when it is cooled down to -60ºC, which is significantly lower than the homogeneous ice nucleation temperature [Bronshteyn, V.L. et al. Biophys. J. 65 (1993) 1853].

Keywords: Wheat photosynthetic lamellae; Membrane lyophilizate; Bound water fractions; Bound water freezing; Bound water overcooling; 1H-NMR relaxometry; 1H-NMR spectroscopy; FID moment expansion; Thylakoid lyophilizate molecular mobility; CPMG

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Received: 2015-3-19
Accepted: 2015-9-16
Published Online: 2016-3-5
Published in Print: 2015-12-1

© University of Wroclaw, Poland

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https://doi.org/10.1515/cmble-2015-0040
Keywords for this article
Wheat photosynthetic lamellae; Membrane lyophilizate; Bound water fractions; Bound water freezing; Bound water overcooling; 1H-NMR relaxometry; 1H-NMR spectroscopy; FID moment expansion; Thylakoid lyophilizate molecular mobility; CPMG

Articles in the same Issue

  1. How taste works: cells, receptors and gustatory perception
  2. Non-cooperative immobilization of residual water bound in lyophilized photosynthetic lamellae
  3. Coexistence of rare variant HbD Punjab [α2β2121(Glu→Gln)] and alpha 3.7 kb deletion in a young boy of Hindu family in West Bengal, India
  4. Somatic stem cell aging and malignant transformation – impact on therapeutic application
  5. 1H NMR-based metabolic profiling for evaluating poppy seed rancidity and brewing
  6. Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: size does not matter; quality control of targeted clones does
  7. Death domain associated protein (Daxx), a multi-functional protein
  8. In silico screening of alleged miRNAs associated with cell competition: an emerging cellular event in cancer
  9. MGL induces nuclear translocation of EndoG and AIF in caspase-independent T cell death
  10. Cerivastatin represses atherogenic gene expression through the induction of KLF2 via isoprenoid metabolic pathways
  11. Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells
  12. Purinergic signaling and the functioning of the nervous system cells
  13. The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes
  14. Ramipril inhibits high glucose-stimulated up-regulation of adhesion molecules via the ERK1/2 MAPK signaling pathway in human umbilical vein endothelial cells
  15. The effect of nicotine on the expressions of the α7 nicotinic receptor gene and Bax and Bcl-2 proteins in the mammary gland epithelial-7 breast cancer cell line and its relationship to drug resistance
  16. The cloning, expression and purification of recombinant human neuritin from Escherichia coli and the partial analysis of its neurobiological activity
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