Ramipril inhibits high glucose-stimulated up-regulation of adhesion molecules via the ERK1/2 MAPK signaling pathway in human umbilical vein endothelial cells
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Moo Hyun Kim
Abstract
Ramipril has recently been shown to have anti-atherogenic properties. However, the specific mechanisms underlying these effects remain unclear. The purpose of this study was to determine the effects of ramipril on induction of adhesion molecules in human umbilical vein endothelial cells (HUVECs) using high-glucose (HG) conditions and to investigate possible underlying molecular mechanisms. The effects of ramipril on expression of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1 production, and ERK phosphorylation were examined in HG-induced HUVECs with inhibitors targeting the mitogen-activated protein kinase (MAPK) signaling pathway. HG induced the expression of the adhesion molecules ICAM-1 and VCAM-1. Pretreatment with ramipril drastically inhibited ICAM-1 and VCAM-1 production in a time- and dose-dependent manner. Moreover, upon investigating the effects of ramipril on the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway, we found that ramipril completely inhibited HG-induced phosphorylation of ERK1/2. ERK inhibitors completely prevented the inhibitory effect of HG. This study demonstrated that ramipril reduces HG-stimulated induction of ICAM-1 and VCAM-1 expression via MAPK signaling, which may be useful for inhibition of atherosclerosis.
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Articles in the same Issue
- How taste works: cells, receptors and gustatory perception
- Non-cooperative immobilization of residual water bound in lyophilized photosynthetic lamellae
- Coexistence of rare variant HbD Punjab [α2β2121(Glu→Gln)] and alpha 3.7 kb deletion in a young boy of Hindu family in West Bengal, India
- Somatic stem cell aging and malignant transformation – impact on therapeutic application
- 1H NMR-based metabolic profiling for evaluating poppy seed rancidity and brewing
- Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: size does not matter; quality control of targeted clones does
- Death domain associated protein (Daxx), a multi-functional protein
- In silico screening of alleged miRNAs associated with cell competition: an emerging cellular event in cancer
- MGL induces nuclear translocation of EndoG and AIF in caspase-independent T cell death
- Cerivastatin represses atherogenic gene expression through the induction of KLF2 via isoprenoid metabolic pathways
- Proteasomes raise the microtubule dynamics in influenza A (H1N1) virus-infected LLC-MK2 cells
- Purinergic signaling and the functioning of the nervous system cells
- The effect of cultureware surfaces on functional and structural components of differentiated 3T3-L1 preadipocytes
- Ramipril inhibits high glucose-stimulated up-regulation of adhesion molecules via the ERK1/2 MAPK signaling pathway in human umbilical vein endothelial cells
- The effect of nicotine on the expressions of the α7 nicotinic receptor gene and Bax and Bcl-2 proteins in the mammary gland epithelial-7 breast cancer cell line and its relationship to drug resistance
- The cloning, expression and purification of recombinant human neuritin from Escherichia coli and the partial analysis of its neurobiological activity
