Startseite Simultaneous analysis of polar and non-polar components of cell membrane phospholipids by GC-MS
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Simultaneous analysis of polar and non-polar components of cell membrane phospholipids by GC-MS

  • Václav M. Bierhanzl EMAIL logo , Miroslava Bursová , Martin Ston , Radomír Čabala und Gabriela Seydlová
Veröffentlicht/Copyright: 25. Juni 2016
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 70 Heft 10

Abstract

A rapid and selective GC-MS method was optimised for the simultaneous analysis of fatty acids, phosphoethanolamine, phosphoglycerol and phosphoserine, which comprise the polar headgroups of phospholipids. Enzymatic hydrolysis was used for sample preparation to selectively release fatty acids and prevent cleavage of the phosphoester bonds of the polar headgroups. A two-step consecutive derivatisation of the fatty acids and polar headgroups was applied to enable the simultaneous analysis of the selected analytes. The method was tested on samples of cell membrane phospholipids of Bacillus subtilis, a microbe with a broad spectrum of fatty acids. This approach can accelerate and simplify biotechnological research and quality control. The experimental conditions were optimised using a chemometric approach denoted as experimental design.

Keywords: bacterial membrane; branched-chain fatty acids; GC-MS; phospholipids; lipidomics; silylation

Acknowledgements

This work receive financial support from grant nos. SVV260317 and 13-18051P from the Czech Science Foundation.

Supplementary data

Supplementary data associated with this article can be found in the online version of this paper (DOI: 10.1515/chempap-2016-0073).

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Received: 2015-9-17
Revised: 2016-3-10
Accepted: 2016-3-11
Published Online: 2016-6-25
Published in Print: 2016-10-1

© 2016 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2016-0073
Schlagwörter für diesen Artikel
bacterial membrane; branched-chain fatty acids; GC-MS; phospholipids; lipidomics; silylation

Artikel in diesem Heft

  1. Original Paper
  2. Simultaneous analysis of polar and non-polar components of cell membrane phospholipids by GC-MS
  3. Original Paper
  4. Cloud point extraction of disulfiram for its HPLC-MS/MS determination in synthetic urine
  5. Original Paper
  6. Revealing the seed proteome of the health benefitting grain amaranth (Amaranthus cruentus L.)
  7. Original Paper
  8. Possible role of hydrolytic enzymes (Sap, Kex2) in Candida albicans response to aromatic compounds bearing a sulfone moiety
  9. Original Paper
  10. Using nutritional and oxidative stress to increase content of healthbeneficial fatty acids in oleaginous and non-oleaginous yeasts
  11. Original Paper
  12. Fatty acids and amino acids of entomopathogenic fungus Conidiobolus coronatus grown on minimal and rich media
  13. Original Paper
  14. Promotional effect of cobalt addition on catalytic performance of Ce0.5Zr0.5O2 mixed oxide for diesel soot combustion
  15. Original Paper
  16. Microwave-assisted continuous reactive distillation process for preparation of ethyl acetate
  17. Original Paper
  18. Wall-retardation effects on particles settling through non-Newtonian fluids in parallel plates
  19. Original Paper
  20. Microwave-assisted decomposition of fgd gypsum in the presence of magnetite and anthracite
  21. Original Paper
  22. Effect of PHB on the properties of biodegradable PLA blends
  23. Original Paper
  24. Thiophene-free diphenyl-amino-stilbene-diketo-pyrrolo-pyrrole derivatives as donors for organic bulk heterojunction solar cells
  25. Short Communication
  26. UV-induced reduction of Ag+ by diazene sulphonates: new method of metallisation of surfaces
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