Startseite Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity
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Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity

  • Van T. Nguyen EMAIL logo , Michael C. Bowyer , Ian A. van Altena und Christopher J. Scarlett EMAIL logo
Veröffentlicht/Copyright: 2. Februar 2016
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 70 Heft 6

Abstract

Phyllanthus amarus is known as a healing herb which has traditionally been used in the treatment of various diseases such as hepatitis, diabetes and cancer. The extraction parameters have great effects on the extraction efficiency of bioactive compounds and pharmacological activity of the extracts. This study sought to optimise the microwave-assisted extraction parameters for phenolic compounds-enriched extracts and antioxidant capacity from P. amarus using response surface methodology (RSM). The results showed that the optimal microwave-assisted extraction parameters were an extraction time of 30 min, an irradiation time of 14 s min−1 and a ratio of solvent to sample of 150 mL g−1. The total phenolic content, phenolic extraction efficiency, saponin content, 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging capacity, 2,2-diphenyl-1-picryl-hydrazil (DPPH) radical scavenging capacity and ferric reducing antioxidant power of the P. amarus achieved under these optimal parameters were 87.3 mg of gallic acid equivalents (GAE) per gram of dried sample, 69.7 %, 134.9 mg of escin equivalents (EE) per gram of dried sample, 997.8, 604.7 and 437.3 all in mg of trolox equivalents (TE) per gram of dried sample, respectively, which were not significantly different from the predicted values (86.9 mg of GAE per gram of dried sample, 67.3 %, 123.5 mg of EE per gram of dried sample, 1013.3 mg of TE per gram of dried sample, 530.6 mg of TE per gram of dried sample and 423.5 mg of TE per gram of dried sample, respectively). Accordingly, the optimal microwave-assisted extraction parameters of 30 min, 14 s min−1 and 150 mL g−1 are recommended for the extraction of enriched phenolics from P. amarus for potential application in the nutraceutical and pharmaceutical industries.

Keywords: Phyllanthus amarus; phenolic compounds; antioxidant; optimisation; microwave-assisted extraction

Acknowledgements

The authors gratefully acknowledge the financial support received from the Ramaciotti Foundation (ES2012/0104) and the University of Newcastle, Faculty of Science and Information Technology. The authors wish to thank the Vietnamese Government through the Vietnam International Education Development-Ministry of Education and Training (Project 911) and the University of Newcastle for awarding a VIED-TUIT scholarship to Van Tang Nguyen. The authors would also like to thank the Centre of Experiment and Practice at Nha Trang University for its mechanical support.

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Received: 2015-8-21
Revised: 2015-11-16
Accepted: 2015-11-16
Published Online: 2016-2-2
Published in Print: 2016-6-1

© 2016 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2016-0009
Schlagwörter für diesen Artikel
Phyllanthus amarus; phenolic compounds; antioxidant; optimisation; microwave-assisted extraction

Artikel in diesem Heft

  1. Original Paper
  2. A practical approach to non-spectral interferences elimination in inductively coupled plasma optical emission spectrometry
  3. Original Paper
  4. Two 1,8-naphthalimide-based proton-receptor fluorescent probes for pH determination
  5. Original Paper
  6. Fabrication of amperometric cholesterol biosensor based on SnO2 nanoparticles and Nafion-modified carbon paste electrode
  7. Original Paper
  8. Preparation and catalytic performance of quaternary ammonium base resin for methanolysis of natural phosphatidylcholine
  9. Original Paper
  10. Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity
  11. Original Paper
  12. Red clover (Trifolium pratense L.) honey: volatiles chemical-profiling and unlocking antioxidant and anticorrosion capacity
  13. Original Paper
  14. Application of vacuum membrane distillation for concentration of organic solutions‡
  15. Original Paper
  16. Correlations for mixing energy in processes using Rushton turbine mixer‡
  17. Original Paper
  18. Recovery of Au(III) ions by Au(III)-imprinted hydrogel
  19. Original Paper
  20. Initiation behaviour in hydrogenation of pyrolysis gasoline over presulphided Ni-Mo-Zn/Al2O3 catalyst
  21. Original Paper
  22. Methodology considering surface roughness in UV water disinfection reactors
  23. Original Paper
  24. Comparison of changes of basic parameters of asphalt caused by various additives
  25. Original Paper
  26. Effect of carbon nanotube modification on poly (butylene terephthalate)-based composites
  27. Original Paper
  28. Evaluation of influence of selected metal cations on antioxidant activity of extracts from savory (Satureja hortensis)‡
  29. Original Paper
  30. Radical-scavenging activity of glutathione, chitin derivatives and their combination‡
  31. Original Paper
  32. Piroxicam /β-cyclodextrin complex included in cellulose derivatives-based matrix microspheres as new solid dispersion-controlled release formulations
  33. Original Paper
  34. Avobenzone encapsulated in modified dextrin for improved UV protection and reduced skin penetration
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  36. Analysis of the dynamics of laser induced plume propagation from liquid matrix using fast photography
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