Startseite Preparation and characterisation of gelatine hydrogels predisposed to use as matrices for effective immobilisation of biocatalystst‡
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Preparation and characterisation of gelatine hydrogels predisposed to use as matrices for effective immobilisation of biocatalystst‡

  • Karolina Labus EMAIL logo , Aleksandra Drozd und Anna Trusek-Holownia
Veröffentlicht/Copyright: 11. Februar 2016
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Chemical Papers
Aus der Zeitschrift Chemical Papers Band 70 Heft 5

Physical, enzymatic and chemical methods were used to develop an efficient procedure for preparing gelatine hydrogels of appropriate strength and elastic properties for applications as enzyme carriers. The concentrations of the crosslinking enzyme (transglutaminase), the initial amount of gelatine, the production time and the effect of additional crosslinking with glutaraldehyde were examined. As a result, the following conditions were selected: 0.1 g cm–3 solution of gelatine, 0.01 g cm–3 of transglutaminase (mTGase), a minimum of 2 h incubation at 4°C and an additional step of crosslinking with 1.0 vol. % of glutaraldehyde. Next, the absorption properties and storage stability of hydrogels so obtained were determined. From these results, it was observed that, with the exception of the physical gel, the remaining materials presented a relatively high resistance to hydrolytic degradation and retained their original spatial structure without any visible damages.

The immobilisation experiments indicated gelatine-based hydrogels crosslinked with transglutaminase as suitable for use as matrices for the entrapment of enzymes, which catalyse the conversion of low-molecular mass compounds. In addition to the potential for effective re-use in subsequent batch processes, the essential advantage of the immobilised β-galactosidase obtained in the current study is a marked reduction in its volume under storage conditions of long duration, without any significant decline in catalytic activity.

Keywords: gelatine; hydrogel; enzymatic crosslinking; absorption properties; enzyme immobilisation

‡Presented at the 42nd International Conference of the Slovak Society of Chemical Engineering, Tatranské Matliare, Slovakia, 25–29 May 2015.

Acknowledgements.

This work was performed, in part, within the project of the National Science Centre in Poland no. 2013/11/B/ST8/03672. The authors would also like to thank the Ajinomoto Company (Japan) for their kind donation of the transglutaminase preparation.

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Received: 2015-6-16
Revised: 2015-10-2
Accepted: 2015-10-5
Published Online: 2016-2-11
Published in Print: 2016-5-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2015-0235
Schlagwörter für diesen Artikel
gelatine; hydrogel; enzymatic crosslinking; absorption properties; enzyme immobilisation

Artikel in diesem Heft

  1. Original Paper
  2. Preparation and characterisation of gelatine hydrogels predisposed to use as matrices for effective immobilisation of biocatalystst‡
  3. Original Paper
  4. Photocatalytic reduction of nitro aromatic compounds to amines using a nanosized highly active CdS photocatalyst under sunlight and blue LED irradiation
  5. Original Paper
  6. Synthesis of butyrate using a heterogeneous catalyst based on polyvinylpolypyrrolidone
  7. Original Paper
  8. Behaviour of selected pesticide residues in blackcurrants (Ribes nigrum) during technological processing monitored by liquid-chromatography tandem mass spectrometry
  9. Original Paper
  10. Influence of solvents and novel extraction methods on bioactive compounds and antioxidant capacity of Phyllanthus amarus
  11. Original Paper
  12. Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction
  13. Original Paper
  14. Innovative approach to treating waste waters by a membrane capacitive deionisation system
  15. Original Paper
  16. Liquid—liquid equilibria of ternary systems of 1-hexene/hexane and extraction solvents
  17. Original Paper
  18. Design of extractive distillation process with mixed entraineri‡
  19. Original Paper
  20. Kinetic study of non-reactive iron removal from iron-gall inks
  21. Original Paper
  22. Chemoenzymatic polycondensation of para-benzylamino phenol
  23. Original Paper
  24. Copper corrosion behaviour in acidic sulphate media in the presence of 5-methyl-lH-benzotriazole and 5-chloro-lH-benzotriazole
  25. Original Paper
  26. Synthesis of new 5-bromo derivatives of indole and spiroindole phytoalexins
  27. Original Paper
  28. Design, synthesis and anti-mycobacterial evaluation of some new iV-phenylpyrazine-2-carboxamides
  29. Short Communication
  30. Convenient amidation of carboxyl group of carboxyphenylboronic acids
  31. Short Communication
  32. A novel intramolecular reversible reaction between the hydroxyl group and isobutenylene chain in a cyclophane-type macrocycle
  33. Erratum
  34. Erratum to “Adriana Bakalova, Boryana Nikolova-Mladenova, Rossen Buyukliev, Emiliya Cherneva, Georgi Momekov, Darvin Ivanov: Synthesis, DFT calculations and characterisation of new mixed Pt(II) complexes with 3-thiolanespiro-5′-hydantoin and 4-thio-1H-tetrahydropyranspiro-5′-hydantoin”, Chemical Papers 70 (1) 93–100 (2016)*
  35. Erratum
  36. Erratum to “Martyna Rzelewska, Monika Baczyńska, Magdalena Regel-Rosocka, Maciej Wiśniewski: Trihexyl(tetradecyl)phosphonium bromide as extractant for Rh(III), Ru(III) and Pt(IV) from chloride solutions”, Chemical Papers 70 (4) 454–460 (2016)*
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