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Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC

  • Alina Oana Matei , Florentina Gatea , Eugenia Dumitra Teodor and Gabriel Lucian Radu EMAIL logo
Published/Copyright: February 2, 2016
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Chemical Papers
From the journal Chemical Papers Volume 70 Issue 4

Abstract

The matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF) and micellar electrokinetic chromatography (MEKC) methods were used to identify and quantify five tannins, (+)-catechin, (–)-epigallocatechin, (–)-epigallocatechin gallate, (–)-epicatechin gallate and (–)-epicatechin, from aqueous, ethanolic and acetonic extracts of Calendula officinalis, Hypericum, perforatum, Galium verum and Origanum vulgare. The MALDI-TOF technique was used for screening tannins monomers and oligomers in plant extracts. The sandwich method and matrix 2,5-dihydroxybenzoic acid with a concentration of 10 mg mL–1 in acetonitrile/ultrapure water/trifluoroacetic acid (20 : 80 : 0.1, vol.) were used. The electrophoretic method developed for the separation and quantification of 5 catechins in 15 min exhibited good efficiency and precision, low limits of detection (0.0032–0.0153 μg mL–1) and quantification (0.0096–0.0466 μg mL–1). The correlation coefficients (R2) exceeded 0.9986 and the recovery values ranged between 94.25 % and 102.50 %. The present work provides new information on some of the less studied compounds present in plants frequently used in traditional medicine.

Keywords: tannin; plant extract; MALDI-TOF; MEKC

Acknowledgements.

This work was supported by Partnership in priority areas-PN II programme, developed with the support of MEN-UEFISCDI project no. 202/2014 (NEW-BIOVECT) and NUCLEU-BIODIV-PN 09-360106/2009.

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Received: 2015-4-23
Revised: 2015-7-21
Accepted: 2015-9-9
Published Online: 2016-2-2
Published in Print: 2016-4-1

© 2015 Institute of Chemistry, Slovak Academy of Sciences

Articles in the same Issue

  1. Original Paper
  2. Prevention of degradation of γ-irradiated EPDM using phenolic antioxidants
  3. Original Paper
  4. Differentiation of black, green, herbal and fruit bagged teas based on multi-element analysis using inductively-coupled plasma atomic emission spectrometry
  5. Original Paper
  6. Reaction mechanisms of carbon dioxide methanation
  7. Review
  8. Power consumption and gas–liquid dispersion in turbulently agitated vessels with vertical dual-array tubular coil baffles
  9. Short Communication
  10. Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC
  11. Original Paper
  12. Trihexyl(tetradecyl)phosphonium bromide as extractant for Rh(III), Ru(III) and Pt(IV) from chloride solutions‡
  13. Original Paper
  14. Synthesis of Fe3O4–Ag nanocomposites and their application to enzymeless hydrogen peroxide detection
  15. Original Paper
  16. Possibilities for removal of chlorinated dye Mordant Blue 9 from model waste water‡
  17. Review
  18. Preparation and properties of gelatin films incorporated with N-hydroxysuccinimide-activated end-bit binary acid
  19. Original Paper
  20. Synthesis and properties of novel reusable nano-ordered KIT-5-N-sulfamic acid as a heterogeneous catalyst for solvent-free synthesis of 2,4,5-triaryl-1 H-imidazoles
  21. Short Communication
  22. Prediction of power consumption in mechanically agitated gassed reactor in viscous batch‡
  23. Original Paper
  24. Hydrogel supported chiral imidazolidinone for organocatalytic enantioselective reduction of olefins in water
  25. Original Paper
  26. Continuous synthesis of N-ethylethylenediamine over supported Cu–Zn–La catalysts
  27. Preface
  28. Enzyme-assisted extraction of citrus essential oil
  29. Preface
  30. Experimental investigations of liquid flow in pipe with flat internal baffles
https://doi.org/10.1515/chempap-2015-0222
Keywords for this article
tannin; plant extract; MALDI-TOF; MEKC

Articles in the same Issue

  1. Original Paper
  2. Prevention of degradation of γ-irradiated EPDM using phenolic antioxidants
  3. Original Paper
  4. Differentiation of black, green, herbal and fruit bagged teas based on multi-element analysis using inductively-coupled plasma atomic emission spectrometry
  5. Original Paper
  6. Reaction mechanisms of carbon dioxide methanation
  7. Review
  8. Power consumption and gas–liquid dispersion in turbulently agitated vessels with vertical dual-array tubular coil baffles
  9. Short Communication
  10. Tannins analysis from different medicinal plants extracts using MALDI-TOF and MEKC
  11. Original Paper
  12. Trihexyl(tetradecyl)phosphonium bromide as extractant for Rh(III), Ru(III) and Pt(IV) from chloride solutions‡
  13. Original Paper
  14. Synthesis of Fe3O4–Ag nanocomposites and their application to enzymeless hydrogen peroxide detection
  15. Original Paper
  16. Possibilities for removal of chlorinated dye Mordant Blue 9 from model waste water‡
  17. Review
  18. Preparation and properties of gelatin films incorporated with N-hydroxysuccinimide-activated end-bit binary acid
  19. Original Paper
  20. Synthesis and properties of novel reusable nano-ordered KIT-5-N-sulfamic acid as a heterogeneous catalyst for solvent-free synthesis of 2,4,5-triaryl-1 H-imidazoles
  21. Short Communication
  22. Prediction of power consumption in mechanically agitated gassed reactor in viscous batch‡
  23. Original Paper
  24. Hydrogel supported chiral imidazolidinone for organocatalytic enantioselective reduction of olefins in water
  25. Original Paper
  26. Continuous synthesis of N-ethylethylenediamine over supported Cu–Zn–La catalysts
  27. Preface
  28. Enzyme-assisted extraction of citrus essential oil
  29. Preface
  30. Experimental investigations of liquid flow in pipe with flat internal baffles
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