Analytical procedure for steroid profiling valid for Athlete Biological Passport
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Anna Jarek
Abstract
Although various attempts have been made to eliminate doping in sport, hitherto they all have proved futile. Moreover, the main class of substances that jeopardises the fair play rule remains the same - anabolic androgenic steroids (AAS). To date, longitudinal monitoring of the fluctuations of the endogenous steroids content for a given athlete is regardeded as the most effective approach to the detection of AAS abuse. This is based on the fact that the activity of the steroid biosynthesis pathway may undergo significant changes in response to the AAS administration. This paper presents the entire analytical procedure for quantification of steroids crucial for the Athlete Biological Passport (ABP): testosterone, epitestosterone, dehydroepiandrosterone, androsterone, etiocholanolone, 5-α-androstandiol and 5-β-androstandiol. The procedure consists of a four-step sample preparation process followed by analysis by gas chromatography coupled with mass spectrometry. The limits of quantification for the substances listed above were; 0.44 ng mL−1, 2.07 ng mL−1, 1.24 ng mL−1, 62.49 ng mL−1, 36.20 ng mL−1, 16.90 ng mL−1 and 14.92 ng mL-1, respectively. Aqueous solutions containing deuterated and non-deuterated steroids were used for calibration purposes. Subsequently, the validation parameters, e.g., precision, accuracy and recovery were evaluated for each substance individually.
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© 2015 Institute of Chemistry, Slovak Academy of Sciences
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Artikel in diesem Heft
- Selection and design of ionic liquids as solvents in extractive distillation and extraction processes
- Analytical procedure for steroid profiling valid for Athlete Biological Passport
- Fabrication of paper-based analytical device by silanisation of filter cellulose using alkyltrimethoxysilane coupled with UV radiation
- Synthesis, characterisation and photocatalytic activity of Ag+- and Sn2+-substituted KSbTeO6
- Dysprosium pertraction through facilitated supported liquid membrane using D2EHPA as carrier
- Volatile compounds composition and antioxidant activity of bee pollen collected in Lithuania
- Self-penetrating and interpenetrating 3D metal–organic frameworks constructed from 4-(4-carboxyphenoxy)-phthalic acid and N-donor auxiliary ligands
- Preparation of ceramic γ-Al2O3–TiO2 nanofiltration membranes for desalination
- Promoting effect of group VI metals on Ni/MgO for catalytic growth of carbon nanotubes by ethylene chemical vapour deposition
- Microwave-assisted solvent-free synthesis and luminescence properties of 2-substituted-4,5-di(2-furyl)-1H-imidazoles
- Synthesis of potential inhibitors of glycosyltransferases representing UDP-GlcNAc
- Development of transition state analogue inhibitors for N-acetylglycosyltransferases bearing D-psicoor D-tagatofuranose scaffolds
- Efavirenz–eudragit E-100 nanoparticle-loaded aerosol foam for sustained release: in-vitro and ex-vivo evaluation
- Photochromic and molecular switching behaviour of Schiff base-containing pyrazolone ring
- Improvements to CO2 headspace biodegradability test
- Synthesis of corn rootworm pheromones from commercial diols
