Artikel
Lizenziert
Nicht lizenziert
Erfordert eine Authentifizierung
Comparison of a commercial urinary neopterin radioimmunoassay with high performance liquid chromatography
-
Angelika Hammerer-Lercher
,
Christina Moser
Veröffentlicht/Copyright:
14. Januar 2012
Abstract
Background: The determination of urinary neopterin using high performance liquid chromatography (HPLC) has routinely been performed in Innsbruck since the middle of the 1980s. The aim was to compare the HPLC method with a commercially available radioimmunoassay.
Methods: Neopterin to creatinine ratios were calculated in the urine of 33 healthy volunteers (33±7.3 years) collected at eight given time points. Neopterin and creatinine were simultaneously determined by HPLC and by radioimmunoassay (RIA; BRAHMS; neopterin) and an enzymatic method (Roche; creatinine), respectively. Assays were compared with Bland-Altman plots and Passing-Bablok regression analysis.
Results: Neopterin to creatinine ratios ranged from 62 to 490 μmol/mol (HPLC) and from 17 to 425 μmol/mol (RIA-enzymatic combined). The ratios were on average 17.3% lower using the combined RIA-enzymatic method compared to the HPLC method. Neopterin concentrations measured by RIA were only slightly lower (on average 4.9%) than by the HPLC and the regression analysis revealed a good accordance with linearity. In contrast, creatinine concentrations by the enzymatic method were on average 12.8% higher than by HPLC.
Conclusions: There is a marked bias between HPLC and the combined RIA-enzymatic method for measurement of urinary neopterin to creatinine ratios which has to be considered when methods are switched during the follow-up of patients.
Received: 2011-8-24
Accepted: 2011-12-16
Published Online: 2012-01-14
Published in Print: 2012-06-01
©2012 by Walter de Gruyter Berlin Boston
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Sie haben derzeit keinen Zugang zu diesem Inhalt.
Artikel in diesem Heft
- Masthead
- Masthead
- Editorial
- Laboratory maternal-fetal medicine: challenges and perspectives
- Reviews
- Candidate biochemical markers for screening of pre-eclampsia in early pregnancy
- Linking preeclampsia and cardiovascular disease later in life
- Mini Reviews
- Fetal nucleic acids in maternal blood: the promises
- Ethical aspects of the expansion of neonatal screening programme due to technological advances
- Prenatal screening for trisomy 21: recent advances and guidelines
- Ultrasound markers for the detection of women at risk of developing pre-eclampsia
- Are biological markers relevant for the diagnosis and the prognosis of preterm premature rupture of membranes (PPROM)?
- Genetics and Molecular Diagnostics
- Study of FTMT and ABCA4 genes in a patient affected by age-related macular degeneration: identification and analysis of new mutations
- Rapid detection of CFH (p.Y402H) and ARMS2 (p.A69S) polymorphisms in age-related macular degeneration using high-resolution melting analysis
- Unlabeled-probe high-resolution melting to detect KRAS codon 12 and 13 mutations in pancreatic adenocarcinoma tissues
- General Clinical Chemistry and Laboratory Medicine
- Volume, rates, source and types of add-on pathology test requests across five hospitals
- Schizophrenia is associated with increased levels of serum Fas and FasL
- Comparison between capillary, venous and arterial levels of protein S100B in patients with severe brain pathology
- Changes of serum adiponectin and soluble intercellular adhesion molecule-1 concentrations after smoking cessation
- Lack of consistency between two commercial ELISAs and against an in-house ELISA for the detection of CD36 in human plasma
- Comparison of a commercial urinary neopterin radioimmunoassay with high performance liquid chromatography
- Evaluation of Prevent ID and Quantum Blue rapid tests for fecal calprotectin
- Analytical performance evaluation of four cartridge-type blood gas analyzers
- Cancer Diagnostics
- Serum free light chains and oligoclonal bands in patients with multiple myeloma and autologous stem cell transplantation
- Cardiovascular Diseases
- Homocysteine as a determinant of left ventricular ejection fraction in patients with diabetes
- Plasma total homocysteine is a determinant of carotid intima-media thickness and circulating endothelial progenitor cells in patients with newly diagnosed hypertension
- Serum MMP-9 and TIMP-1 concentrations and MMP-9 activity during surgery-induced inflammation in humans
- Effects of endothelin-1 on endothelial progenitor cell function
- Investigation of cystatin C and cystatin C based estimated glomerular filtration rate in pregnant patients with heart failure
- Letters to the Editor
- Comments on a performance evaluation of cartridge-type blood gas analyzers
- Comments on a performance evaluation of cartridge-type blood gas analyzers. Reply
- Carryover can be a cause of false-positive results with the Beckman AccuTnI assay
- Method comparison of a 25-hydroxy vitamin D enzyme immunoassay to liquid chromatography tandem mass spectroscopy
- Bone marrow plasma cell separation – validation of separation algorithm
- Sharing our experience in improving postgraduate clinical biochemistry training with laboratory practice articles
Schlagwörter für diesen Artikel
high performance liquid chromatography;
neopterin;
radioimmunoassay;
volunteers
Artikel in diesem Heft
- Masthead
- Masthead
- Editorial
- Laboratory maternal-fetal medicine: challenges and perspectives
- Reviews
- Candidate biochemical markers for screening of pre-eclampsia in early pregnancy
- Linking preeclampsia and cardiovascular disease later in life
- Mini Reviews
- Fetal nucleic acids in maternal blood: the promises
- Ethical aspects of the expansion of neonatal screening programme due to technological advances
- Prenatal screening for trisomy 21: recent advances and guidelines
- Ultrasound markers for the detection of women at risk of developing pre-eclampsia
- Are biological markers relevant for the diagnosis and the prognosis of preterm premature rupture of membranes (PPROM)?
- Genetics and Molecular Diagnostics
- Study of FTMT and ABCA4 genes in a patient affected by age-related macular degeneration: identification and analysis of new mutations
- Rapid detection of CFH (p.Y402H) and ARMS2 (p.A69S) polymorphisms in age-related macular degeneration using high-resolution melting analysis
- Unlabeled-probe high-resolution melting to detect KRAS codon 12 and 13 mutations in pancreatic adenocarcinoma tissues
- General Clinical Chemistry and Laboratory Medicine
- Volume, rates, source and types of add-on pathology test requests across five hospitals
- Schizophrenia is associated with increased levels of serum Fas and FasL
- Comparison between capillary, venous and arterial levels of protein S100B in patients with severe brain pathology
- Changes of serum adiponectin and soluble intercellular adhesion molecule-1 concentrations after smoking cessation
- Lack of consistency between two commercial ELISAs and against an in-house ELISA for the detection of CD36 in human plasma
- Comparison of a commercial urinary neopterin radioimmunoassay with high performance liquid chromatography
- Evaluation of Prevent ID and Quantum Blue rapid tests for fecal calprotectin
- Analytical performance evaluation of four cartridge-type blood gas analyzers
- Cancer Diagnostics
- Serum free light chains and oligoclonal bands in patients with multiple myeloma and autologous stem cell transplantation
- Cardiovascular Diseases
- Homocysteine as a determinant of left ventricular ejection fraction in patients with diabetes
- Plasma total homocysteine is a determinant of carotid intima-media thickness and circulating endothelial progenitor cells in patients with newly diagnosed hypertension
- Serum MMP-9 and TIMP-1 concentrations and MMP-9 activity during surgery-induced inflammation in humans
- Effects of endothelin-1 on endothelial progenitor cell function
- Investigation of cystatin C and cystatin C based estimated glomerular filtration rate in pregnant patients with heart failure
- Letters to the Editor
- Comments on a performance evaluation of cartridge-type blood gas analyzers
- Comments on a performance evaluation of cartridge-type blood gas analyzers. Reply
- Carryover can be a cause of false-positive results with the Beckman AccuTnI assay
- Method comparison of a 25-hydroxy vitamin D enzyme immunoassay to liquid chromatography tandem mass spectroscopy
- Bone marrow plasma cell separation – validation of separation algorithm
- Sharing our experience in improving postgraduate clinical biochemistry training with laboratory practice articles
