Abstract
The antibacterial activity of ethyl acetate extracts and 95% ethanol extracts from 14 microalgal strains against human pathogenic bacteria was tested, and four microlalgal strains were selected for their high biomass productivity and strong antibacterial activity. Subsequently, these four strains (C14, C74, C79, and SY01) were identified as Chlorella sp., Chlorella sorokiniana, Scenedesmus armatus and Chlorella sp., respectively through BLAST analysis of homologous sequences. Additionally, three flocculants including non-ionic polyacrylamide, ferric chloride and chitosan were used to investigate the flocculation of Chlorella sorokiniana C74. The highest flocculation efficiency (96.7 ± 0.7%) was obtained with the chitosan at a dosage of 12 mg/L. Besides, the results showed that the non-ionic polyacrylamide had little influence on flocculation efficiency. None of these three flocculants had effect on the microalgal cell integrity through observing under the microscope. Basically, flocculation with chitosan was feasible for harvesting C. sorokiniana C74.
Abbreviations
- ADM
agar diffusion method
- BBM
Bold–s basal medium
- DMSO
dimethyl sulfoxide
- ITS
internal transcribed spacer
- NPAM
non-ionic polyacrylamide
Acknowledgements
The authors acknowledge the technical assistance of Analytical and Testing Center of Hainan University. This work was supported by the National Science and Technology Support Program of China (2011BAD14B01), the Provincial Science and Technology Program on Modernization of Traditional Chinese Medicine of Hainan (ZY201327), and the Innovation Fund Project for Technology Based Firms (13C26244604892).
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© 2016 Institute of Molecular Biology, Slovak Academy of Sciences
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- Section Zoology
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- p53-Fibrinolytic system and acute lung injury
- Section Cellular and Molecular Biology
- The multipotent action of electromagnetic field
- Section Cellular and Molecular Biology
- Prescreening, identification and harvesting of microalgae with antibacterial activity
- Section Botany
- Cloning and molecular characterization of Myb transcription factors from Leymus (Poaceae: Trticeae)
- Section Botany
- Hydrological soil behavior in areas with semi-arid vegetation (Beni Chougrane Mountains, Algeria)
- Section Botany
- Extent and persistence of water repellency in two Iranian soils
- Section Botany
- The impact of the permanent grass cover or conventional tillage on hydraulic properties of Haplic Cambisol developed on paragneiss substrate
- Section Zoology
- Species composition of tetranychoid mites (Acari: Trombidiformes: Prostigmata: Tetranychoidea) in main landscapes of Tehran and modelling ecological niche of Tetranychoidea in main climates of Tehran Province, Iran
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